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Decellularized blood vessel matrix gel, preparation method therefor and applications thereof

A technology of decellularization and blood vessels, which is applied in the field of decellularized vascular matrix gel and its preparation, can solve the problems that the preparation method of decellularized vascular matrix gel has not been reported, and achieve thorough removal effect, appropriate diameter and uniformity Effect

Inactive Publication Date: 2014-04-09
SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no report about the decellularized vascular matrix gel and its preparation method at present.

Method used

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  • Decellularized blood vessel matrix gel, preparation method therefor and applications thereof
  • Decellularized blood vessel matrix gel, preparation method therefor and applications thereof
  • Decellularized blood vessel matrix gel, preparation method therefor and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation and detection of acellular vascular matrix gel (1)

[0046] 1. Preparation of acellular vascular matrix gel

[0047] 1. Preparation of acellular vascular matrix powder

[0048] Purchase the aorta of an adult pig (about 120kg) from a slaughterhouse, remove the outer membrane and fascia, cut it into small pieces of irregular blood vessels, place it in 1% Triton X-100 solution, shake at 37°C, and shake at 200rpm to decellularize After 6.5 days of treatment, wash with water every other day and replace with 1% Triton X-100 solution until the blood vessel pieces turn white. The decellularized blood vessel slices were frozen overnight in a -80°C refrigerator, and then placed in a vacuum freeze-dryer (Labconco Triad 2.5L, the United States) for freeze-drying at a temperature of -50°C for about 48 hours until the blood vessel slices became dry. Dry and hard. Then use a small pulverizer to crush it into powder, and filter through a 60-mesh sieve.

[00...

Embodiment 2

[0073] Example 2 Preparation and detection of acellular vascular matrix gel (2)

[0074] 1. Preparation of acellular vascular matrix gel

[0075] 1. Preparation of acellular vascular matrix powder

[0076] Purchase the aorta of an adult pig (about 120kg) from a slaughterhouse, remove the outer membrane and fascia, cut it into small pieces of irregular blood vessels, place it in 0.9% Triton X-100 solution, shake at 37°C, shake at 200rpm, and decellularize After 7 days of treatment, wash with water every other day and replace with 0.9% Triton X-100 solution until the blood vessels turn white. The blood vessel slices after decellularization were placed in a -80°C refrigerator overnight, and then placed in a vacuum freeze-dryer (Labconco Triad 2.5L, the United States) for freeze-drying at a temperature of -50°C for about 46 hours until the blood vessel slices became Dry and hard. Then use a small pulverizer to crush it into powder, and filter through a 60-mesh sieve.

[0077...

Embodiment 3

[0094] Example 3 Preparation and detection of acellular vascular matrix gel (3)

[0095] 1. Preparation of acellular vascular matrix gel

[0096] 1. Preparation of acellular vascular matrix powder

[0097] Purchase the aorta of adult pigs (about 120kg) from the slaughterhouse, remove the adventitia and fascia, cut into small pieces of irregular blood vessels, place them in 1.1% Triton X-100 solution, shake at 37°C, and shake at 200rpm to decellularize After 6 days of treatment, wash with water every other day and replace with 1.1% Triton X-100 solution until the blood vessel pieces turn white. The decellularized blood vessel slices were frozen overnight in a -80°C refrigerator, and then placed in a vacuum freeze-dryer (Labconco Triad 2.5L, the United States) for freeze-drying at a temperature of -50°C for about 50 hours until the blood vessel slices became Dry and hard. Then use a small pulverizer to crush it into powder, and filter through a 60-mesh sieve.

[0098] 2. P...

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Abstract

The invention relates to a kind of decellularized blood vessel matrix gel, a preparation method therefor and applications thereof. The preparation method is as follows: first, after blood vessel outer membranes and anadesma are removed, the blood vessels are cut into blood vessel pieces, the blood vessel pieces are subjected to decellularized treatment in a TritonX-100 solution, then subjected to freeze drying, smashed into powder, and filtered with a 60-mesh screen, and decellularized blood vessel matrix powder is obtained; second, the decellularized blood vessel matrix powder is placed in a hydrochloric acid solution containing pepsin with a concentration of 0.09-0.11%, and subjected to digestion for 66-78h, a sodium hydroxide solution is added to neutralize hydrochloric acid, then PBS is added to balance ion concentration, finally, the gel solution is placed in an incubator with a temperature of 37 DEG C for gel forming. The decellularized blood vessel matrix gel has good molding capability and mechanical properties, the interior is loose, the gel has certain poriness, and the collagen fiber has appropriate diameters and good stability. The gel can meet requirements of preparation of medicines treating ischemic diseases, support materials of tissue engineering and / or cell culture.

Description

technical field [0001] The invention relates to the technical field of tissue engineering gel, in particular to a decellularized vascular matrix gel and its preparation method and application. Background technique [0002] Fibrin gel is a good scaffold material and has always been one of the focuses of tissue engineering research. Fibrin gel is mainly composed of natural extracellular matrix components, has good biocompatibility, effective bioactivity and biodegradability, and also has a three-dimensional porous structure and good plasticity. In recent years, fibrin gel has been widely used in tissue engineering research as a scaffold material, which plays an important role in the formation of muscle tissue, bone and cartilage tissue, epithelial tissue, etc. Sufficient mechanical strength. [0003] Acellular vascular matrix gel is a kind of tissue engineering gel, which can be used in the following fields: 1) It is used for injection to directly treat ischemic diseases: su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/44A61K9/06A61L27/36C12N5/071A61P9/10
Inventor 付炜冯蓓殷猛王伟
Owner SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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