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Streptavidin functionalized semiconductor nano material-based tumor marker electrochemical immunosensor and preparation method thereof

A technology of streptavidin and tumor markers, applied in nanotechnology for sensing, scientific instruments, nanotechnology, etc., can solve the problems of high detection limit of samples and inability to meet the detection of low-concentration tumor markers

Inactive Publication Date: 2014-03-19
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The relatively fast and simple electrochemical immunoassay cannot meet the detection of low-concentration tumor markers due to the high detection limit of samples

Method used

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  • Streptavidin functionalized semiconductor nano material-based tumor marker electrochemical immunosensor and preparation method thereof
  • Streptavidin functionalized semiconductor nano material-based tumor marker electrochemical immunosensor and preparation method thereof
  • Streptavidin functionalized semiconductor nano material-based tumor marker electrochemical immunosensor and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Schematic diagram of immunosensor preparation and immunoassay figure 1 :

[0025] (1) Synthesize strontium titanate, a semiconductor nanoparticle material, disperse it in a chitosan solution, and then add streptavidin to carry out biological functionalization.

[0026] (2) The glassy carbon electrode used was polished with 0.05mm alumina powder, the residual alumina powder was rinsed with deionized water, ultrasonically cleaned in dilute nitric acid solution, and finally the electrode surface was cleaned with ethanol and double distilled water in sequence.

[0027] (3) Take the mixed solution of strontium titanate / chitosan / streptavidin and apply it dropwise on the surface of the pretreated electrode. Alpha-fetoprotein immunosensor was obtained.

[0028] (4) Place the prepared α-fetoprotein immunosensor in the detection system, add α-fetoprotein sample and enzyme-labeled antibody to incubate at room temperature, after washing, use thionine and hydrogen peroxide as enzy...

Embodiment 2

[0032] (1) Synthesize tin sulfide, a semiconductor nanosheet material, disperse it in a chitosan solution, and then add streptavidin to carry out biological functionalization.

[0033] (2) The glassy carbon electrode used was polished with 0.05mm alumina powder, the residual alumina powder was rinsed with deionized water, ultrasonically cleaned in dilute nitric acid solution, and finally the electrode surface was cleaned with ethanol and double distilled water in sequence.

[0034] (3) Take the mixed solution of tin sulfide / chitosan / streptavidin and apply it dropwise on the surface of the pretreated electrode. Carcinoembryonic antigen immunosensor.

[0035] (4) Place the prepared carcinoembryonic antigen immunosensor in the detection system, add carcinoembryonic antigen samples and enzyme-labeled antibodies to incubate at room temperature, wash, use thionine and hydrogen peroxide as enzyme reaction substrates, and use three electrodes The system detects its current signal. D...

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Abstract

The invention relates to a streptavidin functionalized semiconductor nano material-based tumor marker electrochemical immunosensor and a preparation method thereof. The immunosensor is prepared by the following steps: firstly, synthesizing or screening the semiconductor nano structure material with excellent performance, carrying out bio-functionalization by using the streptavidin, and then specifically combining with a biotinylation antibody. The prepared sensor is incubated in a mixed solution containing an enzyme-labeled antibody and an antigen, thionine and hydrogen peroxide are taken as enzyme reaction substrates, and the tumor marker is detected by using a sandwich immunoassay method. An ampere response of an electrode is increased along with an increase of antigen concentration in an incubation solution. The detection method is high in sensitivity, low in detection limit, and good in reproducibility and stability, and can be applied to quantitative determination of the tumor marker in serum.

Description

technical field [0001] The invention relates to an electrochemical immunosensor for tumor markers based on streptavidin functionalized semiconductor nanomaterials, which synthesizes semiconductor nanomaterials and utilizes streptavidin to functionalize them to construct a novel streptavidin-based sensor. The invention relates to the detection of tumor markers by an electrochemical immunoassay method of mycoavidin functionalized semiconductor nanometer materials, which belongs to the technical field of electrochemical immunoassay. Background technique [0002] Tumor is a disease with high morbidity and high mortality that seriously threatens human health. A large number of research and prevention data have confirmed that early diagnosis and early treatment are the most effective ways to prevent and treat tumors and reduce mortality. Therefore, searching for tumor markers for early diagnosis has become the focus of attention for a long time. Tumor markers are certain substanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/561B82Y15/00
CPCG01N33/54366B82Y15/00G01N33/54353
Inventor 杨占军汤艳李娟
Owner YANGZHOU UNIV
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