Lipidosome encapsulating epigallocatechin gallate and preparation method thereof
A technology for epigallocatechin and gallate, which is applied in the field of liposomes and preparations for encapsulating epigallocatechin gallate, and can solve the problems of prone to oxidation, polymerization, condensation, low bioavailability, Low bioavailability and other problems, to achieve the effect of easy popularization and application, low equipment requirements, and simple preparation process
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Embodiment 1
[0025] Dissolve 90mg of soybean lecithin and 30mg of cholesterol in a mixed solution containing 6ml of chloroform and 9ml of ether to obtain liquid A, dissolve 15mg of EGCG in 3ml of phosphate buffer solution to obtain liquid B, and mix the two solutions of liquid A and liquid B , first magnetically stirred for 1.5h, and then ultrasonicated for 5min under the condition of a water bath sonicator with a frequency of 100Hz, so that the mixed solution became a clear single phase; in a water bath at 40°C, under a vacuum pressure of 0.09 Mpa, the mixed solution was rotary evaporated until Form a layer of lipid film; add 300 μl Tween-80 and 30 ml phosphate buffer solution to the lipid film to dissolve the lipid film to obtain solution C; leave solution C at room temperature for 2 hours, and then pass through 0.8 μm Filter paper, 0.45 μm filter membrane, and store the filtrate in a refrigerator at 4°C to obtain liposomes encapsulating EGCG.
[0026] Phosphate buffer configuration:
...
Embodiment 2
[0033] Dissolve 80mg of soybean lecithin and 40mg of cholesterol in a mixed solution containing 6ml of chloroform and 9ml of ether to obtain liquid A, dissolve 18mg of EGCG in 3ml of phosphate buffer solution to obtain liquid B, and mix the two solutions of liquid A and liquid B , first magnetically stirred for 1.5h, and then ultrasonicated for 5min under the condition of a water bath sonicator with a frequency of 100Hz, so that the mixed solution became a clear single phase; then the mixed solution was rotary evaporated in a water bath at 40°C under a vacuum pressure of 0.09 Mpa Until a layer of lipid film is formed; add 300 μl Tween-80 and 30 ml phosphate buffer solution to the lipid film to dissolve the lipid film to obtain solution C; leave solution C at room temperature for 2 hours, and then pass through 0.8 μm filter paper, 0.45 μm filter membrane, and store the filtrate in a refrigerator at 4°C to obtain EGCG-encapsulated liposomes.
[0034] The configuration of the pho...
Embodiment 3
[0036] Dissolve 100mg of soybean lecithin and 20mg of cholesterol in a mixed solution containing 6ml of chloroform and 9ml of ether to obtain liquid A, dissolve 13mg of EGCG in 3ml of phosphate buffer solution to obtain liquid B, and mix the two solutions of liquid A and liquid B , first magnetically stirred for 1.5h, and then ultrasonicated for 5min under the condition of a water bath sonicator with a frequency of 100Hz, so that the mixed solution became a clear single phase; in a water bath at 40°C, under a vacuum pressure of 0.09 Mpa, the mixed solution was rotary evaporated until Form a layer of lipid film; add 300 μl Tween-80 and 30 ml phosphate buffer solution to the lipid film to dissolve the lipid film to obtain solution C; leave solution C at room temperature for 2 hours, and then pass through 0.8 μm Filter paper, 0.45 μm filter membrane, and store the filtrate in a refrigerator at 4°C to obtain liposomes encapsulating EGCG.
[0037] The configuration of the phosphate...
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