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Collection and storage method of cordyceps militaris ascospores

A technology of ascospores and grass ascus, which is applied in the field of collection and preservation of ascospores of Cordyceps militaris, can solve the problems of inability to collect and complicated operation, and achieve the effects of saving manpower and material resources, simple operation and maintaining biological activity

Active Publication Date: 2014-02-12
BEIJING ZHONGYI YUANDA MECHANICAL & ELECTRICAL EQUIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are two deficiencies in the above method: the first, complicated operation
Second, only mature ejected spores can be collected, while mature spores that have not been ejected cannot be collected

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Cultivation of Cordyceps militaris fruiting bodies

[0033] 1. Strain: The strain number obtained by independent screening is HX-64 Cordyceps militaris strain (purchased from the Biological Center of Beijing Academy of Agriculture and Forestry).

[0034] 2. Medium for liquid shake flask (1L): peeled potatoes 300g (boiled juice), glucose 10g, peptone 2g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 1g, water 1000ml, pH 7.2.

[0035] 3. Nutrient solution for fruit body production: 2% soybean, 1% glucose, 5% milk powder, 0.5% potassium dihydrogen phosphate, 0.05% magnesium sulfate, and vitamin B110mg / 1000ml to prepare a nutrient solution, and adjust the pH to 5.6-7.2.

[0036] 4. A culture box made of PP material (10cm in height, 12cm in diameter at the upper bottom, and 8cm in diameter at the bottom). Each box of rice 40g and fruit body production nutrient solution 56g are packaged and mixed, sterilized and inoculated.

[0037] 5. Mycelium culture ma...

Embodiment 2

[0042] Example 2 Collection and preservation of ascospores of Cordyceps militaris (1)

[0043] (1) Preparation of polyspore carrier for adsorption of sand:

[0044] Sieve the river sand with 60 mesh, discard large particles and impurities, and then sieve with 80 mesh to remove fine sand. Absorb the iron with a magnet, put it in a container and soak it in 10% hydrochloric acid, pour off the hydrochloric acid after 24 hours, wash it with water for several times until it becomes neutral, and dry the sand.

[0045] (2) Sterilization and drying of polyspore collection container:

[0046] Take about 25 g of the treated polyspore carrier adsorption sand and mix it with an equal amount of distilled water, put it into a 250 mL conical flask, and then sterilize the whole with moist heat at 121 °C for 30 min. After sterilization, put it in a drying oven at 80°C for 24 hours until it is completely dry.

[0047] (3) Selection of parental fruiting bodies for the separation of polyspores:...

Embodiment 3

[0056] Example 3 Collection and preservation of ascospores of Cordyceps militaris (2)

[0057] (1) Preparation of polyspore carrier for adsorption of sand:

[0058] Sieve the river sand with 60 mesh, discard large particles and impurities, and then sieve with 80 mesh to remove fine sand. Absorb the iron with a magnet, put it in a container and soak it in 15% hydrochloric acid, pour off the hydrochloric acid after 24 hours, wash and soak with water for several times until it becomes neutral, and dry the sand.

[0059] (2) Sterilization and drying of polyspore collection container:

[0060] Take about 50 g of the treated polyspore carrier adsorption sand and mix it with an equal amount of distilled water, put it into a 250 mL conical flask, and then sterilize the whole by moist heat at 121 °C for 30 min. After sterilization, put it in a drying oven at 80°C for 48 hours until it is completely dry.

[0061] (3) Selection of parental fruiting bodies for the separation of polyspo...

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Abstract

The invention provides a collection and storage method of cordyceps militaris ascospores. The collection method comprises the following steps: adding sand into a multi-spore separating and collecting container which can be sealed; sterilizing and drying; putting the selected matured sporocarp of cordyceps militaris ascocarps into the multi-spore separating and collecting container containing the sand; vibrating the multi-spore separating and collecting container so as to collect the sand containing matured ascospores which are ejected out or not; sealing the collected sand in a dry and sterile container; and preserving the container at room temperature by keeping out of the sun or in a refrigerator in a cold storage manner. According to the collection method, the operation is simple and the ascospore collecting rate is increased; the preserved ascospores can retain the inheritable characters of the original parents of cordyceps militaris after being activated and novel breeds for stress resistance can be screened.

Description

technical field [0001] The invention belongs to the technical field of Cordyceps militaris cultivation, in particular to a method for collecting and preserving ascospores of Cordyceps militaris. Background technique [0002] Cordyceps militaris (L.ex Fr) Link, also known as Cordyceps militaris, Cordyceps militaris, parasitic on the pupa of Lepidoptera, Coleoptera, Diptera and other insects. It grows in low-altitude plains, mainly in the northeast, north, and northwest. Modern research shows that Cordyceps militaris contains cordycepin (3'-deoxyadenosine), cordycepin (D-mannitol), adenosine, cordyceps polysaccharide, ergosterol, superoxide dismutase (SOD), selenium (Se), etc. chemical composition. It has the functions of improving human immune function, anti-cancer, anti-bacterial, anti-fatigue, anti-aging, anti-convulsant, hypoxia resistance, sedative, aphrodisiac and kidney-strengthening. At the same time, it also has the effects of weight loss, slimming, beauty and so o...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12N1/04A01G1/04
Inventor 胡健
Owner BEIJING ZHONGYI YUANDA MECHANICAL & ELECTRICAL EQUIP
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