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Extraction method of microalgae grease

A technology of microalgae oil and extraction method, which is applied in the direction of fat oil/fat production and fat generation, can solve the problems of limited effect of alcohol dissolution, achieve the effect of increasing yield, improving alcohol dissolution efficiency, and strengthening mass transfer process

Inactive Publication Date: 2014-02-12
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, a short-chain alcohol solvent is added after the microalgae are subjected to microwave treatment to destroy the cells. Although the short-chain alcohol solvent has a certain dissolution effect on the cell membrane phospholipid layer after the cell destruction, the effect of alcohol dissolution is limited, and the short-chain alcohol Mainly involved in base-catalyzed reactions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A kind of chlorella vulgaris was selected and cultured in a 10L tank for 15 days. At the later stage of microalgae culture, according to the established relationship between OD and cell dry weight, the concentration of microalgae was calculated to be 1.02g / L (dry weight count). Take 5L of microalgae culture solution, adjust the pH of the microalgae culture solution to 11.5 with NaOH, let it stand for 30 minutes, take the algae solution from the bottom of the culture tank and centrifuge, control the centrifugal speed at 5000rpm, and centrifuge for 5 minutes.

[0022] (1) Stir and mix the algae mud collected above with methanol and n-hexane, the ratio is: 1:30:50 (g dry weight: ml:ml), and the stirring time is 5 minutes;

[0023] (2) Send the mixed solution in step (1) into the supergravity rotating packed bed, the supergravity level is 20g, the temperature is controlled at 60°C, and the average residence time of the mixed solution in the supergravity rotating packed bed ...

Embodiment 2

[0027] A kind of botrytis (Botryococcus braunii) was selected and cultured in a 10L tank for 20 days. At the later stage of microalgae culture, according to the established relationship between OD and cell dry weight, the concentration of microalgae was calculated to be 1.43g / L (dry weight basis ). Take 3L of microalgae culture solution, adjust the pH of the microalgae culture solution to 12 with NaOH, let it stand for 30 minutes, take the algae solution from the bottom of the culture tank and centrifuge at 10,000 rpm for 10 minutes.

[0028] (1) Stir and mix the algae mud collected above with methanol and n-hexane, the ratio is: 1:20:40 (g dry weight: ml:ml), and the stirring time is 10 minutes;

[0029] (2) Send the mixed solution in step (1) into the supergravity rotating packed bed, the supergravity level is 50g, the temperature is controlled at 65°C, the average residence time of the mixed solution in the supergravity rotating packed bed is 3s, and the cycle operation 2 ...

Embodiment 3

[0032] The cultivated diatoms were centrifuged, and the obtained algae mud was dried in an oven at 60°C for 12 hours.

[0033] (1) Weigh 5g of diatom dry algae powder, stir and mix the algae powder with methanol and petroleum ether, the ratio is: 1:30:60 (g dry weight: ml:ml), stirring time: 15min;

[0034] (2) Send the mixture of algae and solvent in step (1) into the high-gravity rotating packed bed, the super-gravity level is 80g, the temperature is controlled at 70°C, the average residence time of the mixture in the high-gravity rotating packed bed 4s;

[0035](3) After the crushing of microalgae cells and the extraction of oil, cool the effluent in the supergravity rotating packed bed in step (2) to room temperature, let it stand for 20 minutes, and the mixture is divided into three layers, namely, oil phase, water phase Phase and precipitation, after separation, the calculated oil yield is 29.4%.

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Abstract

The invention discloses an extraction method of microalga grease. The method comprises the following steps of: (1) stirring and mixing collected microalgae with a C1-C6 short-chain alcohol solvent and an extractant; (2) adding the mixed liquid into a supergravity crushing device to perform microalgae cell crushing and grease extraction; and (3) after the microalgae cell crushing and the grease extraction are finished, cooling the discharge liquid of the supergravity crushing device to room temperature, permitting the liquid to stand, respectively extracting an organic solvent layer (an oil phase) and a water layer (a water phase), and recovering the organic solvent in the oil phase by a distillation way, and meanwhile, obtaining the microalga grease. The method has the advantages of simple operation processes, short extraction time, the high microalga grease yield, suitability for directly extracting grease from alga ooze, and the like over the prior art.

Description

technical field [0001] The invention relates to a method for purifying microalgae oil, in particular to a method for extracting microalgae oil using supergravity technology. Background technique [0002] Microalgae are a class of lower plants that grow in water and are diverse and widely distributed. This type of organism has an efficient photosynthetic reaction system, which can 2 Light energy is converted into chemical energy and stored in the body cells in the form of organic matter such as oil or starch. With the pressure of shortage of resources in human society and the increasingly serious environmental problems, the development of biodiesel and some fossil energy substitutes using microalgae has become a research hotspot. [0003] The use of microalgae for biodiesel production is a complex system engineering, covering multiple technical links, including screening and cultivation of microalgae species, large-scale cultivation of microalgae and induction of oil produc...

Claims

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Application Information

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IPC IPC(8): C11B1/10C11B1/04
Inventor 李晓姝金平王领民张霖师文静乔凯黎元生
Owner CHINA PETROLEUM & CHEM CORP
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