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Device and method for preparing butanol through fermentation, coupling, separation and purification of acetone-butanol

A technology for the fermentation, separation and purification of acetone butanol, which is applied in the field of biochemical industry to achieve the effects of saving energy consumption, increasing equipment investment and improving efficiency

Active Publication Date: 2014-02-05
NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the separation and purification of butanol and acetone using the gas stripping purification method coupled with osmosis-heating-gas stripping

Method used

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  • Device and method for preparing butanol through fermentation, coupling, separation and purification of acetone-butanol
  • Device and method for preparing butanol through fermentation, coupling, separation and purification of acetone-butanol
  • Device and method for preparing butanol through fermentation, coupling, separation and purification of acetone-butanol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] figure 1 It is a schematic diagram of the device of the present invention. Depend on figure 1 As can be seen, the acetone-butanol fermentation coupling separation and purification device of the present invention prepares butanol, which comprises a seed culture tank 1, a stirred bioreactor 2, a cooling device 3, an immobilization device 4, a bacterial liquid separation device 5, an air stripping Condensation pipe 6, storage tank 7, low-temperature circulation constant temperature tank 8 and condensate collector 9; wherein, the stirred bioreactor 2 is communicated with the seed cultivation tank 1 through a pipeline; the built-in gas distributor at the bottom of the stirred bioreactor 2; stirring Type bioreactor 2, cooling device 3, immobilization device 4, and bacterial liquid separation device 5 are sequentially connected to form a circulation loop; and stirred bioreactor 2 is sequentially connected to stripping condenser pipe 6 and storage tank 7 , the storage tank 7 ...

Embodiment 2

[0031] Inoculate the butanol-producing bacteria into the seed culture tank 1 for seed cultivation; then pump it into the stirred bioreactor 2 for fermentation through the pump 10, and pump the fermented liquid out of the stirred bioreactor 2 through the pump 11 during the fermentation process Enter the cooling device 3 to reduce the temperature of the fermented liquid, continue to pump into the immobilization device 4 to realize the immobilization of the bacterial strain, then pump into the bacterial liquid separation device 5, and the entrapped liquid of the bacterial liquid separation device 5 returns to the immobilization device 4, and the bacterial liquid separation device The effluent of 5 is pumped back into the stirred bioreactor 2 through the pump 12, so as to realize the immobilization of the bacterial strain cells. The effects of cell immobilization are as follows: ① most of the cells are adsorbed by the adsorption and immobilization material, and a small part of the ...

Embodiment 3

[0044] Acetobutanol-producing bacteria: Clostridium acetobutylicum strain (Clostridium acetobutylicum strain), purchased from China General Microbiology Collection Center (CGMCC5234)

[0045] Seed medium: yeast powder 3g / L, peptone 5g / L, glucose 10g / L (usually glucose has a molecule of crystal water, which is actually 10% more), ammonium acetate 2g / L, sodium chloride 2g / L, seven Magnesium sulfate hydrate 3g / L, potassium dihydrogen phosphate 1g / L, dipotassium hydrogen phosphate 1g / L, ferrous sulfate heptahydrate 0.1g / L.

[0046] Fermentation medium: carbon source (glucose 60g / L), phosphate buffer (potassium dihydrogen phosphate 0.5g / L, dipotassium hydrogen phosphate 0.5g / L), ammonium acetate 2.2g / L, vitamins (p-aminobenzoic acid 1mg / L, thiamine 1mg / L, biotin 0.01mg / L), mineral salt (manganese sulfate monohydrate 0.01g / L, sodium chloride 0.01g / L, magnesium sulfate heptahydrate 0.2g / L, sulfuric acid heptahydrate ferrous iron 0.01g / L).

[0047] Cultivation and fermentation of bu...

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Abstract

The invention discloses a device for preparing butanol through the fermentation, coupling, separation and purification of acetone-butanol. A stirred bioreactor is communicated with a seed cultivating pot through a pipeline; a gas distributor is arranged in the bottom of the stirred bioreactor; the stirred bioreactor, a cooling device, an immobilization device and a bacteria liquid separation device are sequentially communicated so as to form a circulation loop; the stirred bioreactor is sequentially communicated with an air stripping condenser pipe and a storage tank, the storage tank is arranged in a low-temperature circulating constant-temperature bath, and the storage tank is communicated with a condensed fluid collector through a pipeline. The invention also discloses a method for preparing butanol through the fermentation, coupling, separation and purification of acetone-butanol by using the device. According to the invention, besides the inhibition effect of sugar and acetone-butanol fermentation products is effectively eliminated, and the yield of acetone-butanol fermentation products and the utilization efficiency of raw materials are improved, the production energy consumption and the cost also can be reduced, therefore, the device and method disclosed by the invention provide a new technical support for the separation and purification of butanol and acetone and products thereof produced by using a biological method currently.

Description

technical field [0001] The invention relates to a device and method for preparing butanol through acetone-butanol fermentation coupling separation and purification, belonging to the field of biochemical industry. Background technique [0002] With the massive consumption of petroleum non-renewable resources, its prices continue to rise, many companies in the world have begun to make strategic adjustments, replace non-renewable resources with bio-fermentation renewable resources, and replace the production of chemical technology routes with biotechnology routes . A nascent bioenergy—butanol, has entered people's field of vision. It is a new fuel with great potential and is called the second-generation biofuel. Compared with other biofuels, the combustion value and octane number of biobutanol are the closest to those of gasoline, and it has obvious advantages in terms of fuel performance and economy. The compatibility between butanol and gasoline is better, and it can achiev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12P7/16C07C31/12C07C29/80C12R1/145C12R1/19
CPCC12M29/08C12M29/18C12M43/02C12M47/02C12P7/16Y02E50/10
Inventor 应汉杰陈勇任恒飞朱晨杰吴菁岚庄伟谢婧婧郭亭
Owner NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD
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