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A method for extracting microbial oil

A microbial grease and oil-producing microorganism technology, applied in the direction of fat production, fat oil/fat production, etc., can solve the problems of low oil quality, not bright enough, heavy smell, etc., achieve low peroxide value, save energy consumption, and acid value low effect

Active Publication Date: 2015-12-30
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microbial oil extracted by this method has a strong smell, dark color, and is not translucent enough, so the quality of the oil is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Using Mortierella alpina as the fermentation strain, follow the steps below:

[0026] 1) Inoculate and ferment Mortierella alpina; add pectinase with a mass of 0.0001 g / mL of fermentation broth volume 1 hour before the end of fermentation;

[0027] 2) The fermented liquid obtained after the fermentation is finished is obtained through a plate-and-frame filter press to obtain wet thallus with a water content of 30wt%;

[0028] 3) Take 1000 g of wet bacteria, add 2000 ml of n-hexane, and crush it twice in a colloid mill until the volume average particle size of the emulsion particles is 800 μm. Pass the bacterial cell mixture through a plate and frame filter press to obtain mixed oil and bacterial meal, and desolventize the mixed oil to obtain polyunsaturated fatty acid ARA oil, with a yield of 85.3 wt%. Among them, the acid value of the oil is 0.56mgKOH / g, the peroxide value is 1.4meq / kg, the yellow value of the color is 4.0, and the red value is 1.0.

Embodiment 2

[0030] Using Mortierella alpina as the fermentation strain, follow the steps below:

[0031] 1) adding pectinase and 0.0001 g / mL protease with a mass of 0.0001 g / mL of fermentation broth volume to Mortierella alpina inoculated and fermented 4 hours before the end of fermentation;

[0032] 2) The fermented liquid obtained after the fermentation is finished is obtained through a plate-and-frame filter press to obtain wet thallus with a water content of 50wt%;

[0033] 3) Take 1000g of wet bacteria, add 3000ml of n-hexane, and crush it 3 times in a shearing machine until the volume average particle size of the emulsion particles is 700μm, and then pass the mixed solution through a plate and frame filter press to obtain an extraction mixed oil and a Bacteria meal;

[0034] 4) Add 2000ml of n-hexane to the first extraction bacteria meal, and circulate and crush it twice in a shearing machine, and then separate it through a plate and frame filter press to obtain the second extracti...

Embodiment 3

[0037] Using Mortierella alpina as the fermentation strain, follow the steps below:

[0038] 1) Mortierella alpina was inoculated and fermented; 8 hours before the end of the fermentation, protease and 0.0002 g / mL pectinase with a mass of 0.0002 g / mL of fermentation broth were added;

[0039] 2) The fermented liquid obtained after the fermentation is finished is obtained through a plate and frame filter press mechanism to obtain wet thallus with a water content of 80wt%;

[0040] 3) Take 1000g of wet bacteria, add 4000ml of petroleum ether, and crush it in a shearing machine for 5 times until the volume average particle size of the emulsion particles is 600 μm, and then pass the mixed solution through a centrifuge to obtain the first-extracted mixed oil and the first-extracted bacterial meal;

[0041] 4) Add 2000ml of petroleum ether to the first-extraction bacteria meal, circulate and crush it twice in a shearing machine, and then separate it by a centrifuge to obtain the sec...

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Abstract

The invention relates to a method of extracting microbial oil. The method comprises the following steps of: carrying out inoculated fermentation on oleaginous microbes; processing fermentation broth obtained after fermentation in a separation system to prepare wet thalli with the water content of 30-80wt percent; uniformly mixing the wet thalli and an organic solvent for extraction, and carrying out circulation crushing until the volume average particle diameter of emulsion particles is less than 800 microns; carrying out solid-liquid separation on the thallus mixed solution after the circulation crushing to obtain mixed oil and thallus meal; and carrying out desolvation on the mixed oil to obtain the microbial oil. With the adoption of the method, a high-temperature drying procedure is not needed, so that numerous deterioration reactions of oil under a high temperature can be greatly lowered, and the energy consumption is also lowered; in addition, as crushing and extraction are carried out synchronously, the oil can be prevented from coming into contact with air to the greatest extent, oxidation of the oil under the state of small particle sizes is avoided, and the purpose of extracting the high-quality microbial oil with low acid value, light color and low peroxide value by virtue of the method is reached.

Description

technical field [0001] The invention relates to a method for extracting microbial oil. Background technique [0002] Polyunsaturated fatty acid (polyunsaturated fatty acid, PUFA) generally refers to a straight-chain fatty acid containing two or more double bonds and a carbon chain length of 18-22. According to the position of the carbon atom where the double bond is located, it is generally divided into omega-3 series unsaturated fatty acids and omega-6 series unsaturated fatty acids. For example, docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) belong to the omega-3 series, while linoleic acid and arachidonic acid (ARA) belong to the omega-6 series. Polyunsaturated fatty acids (PUFA) have many important physiological activities. It can regulate the normal metabolism of blood lipids and lipoproteins in the human body, reduce the content of cholesterol in the blood, and prevent cardiovascular and cerebrovascular diseases. At the same time, it can be converted into ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/00
Inventor 汪志明田勇周强杨刚陆姝欢余超
Owner CABIO BIOTECH WUHAN CO LTD
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