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Chicken infectious bronchitis virus vaccine strain (hf2 strain) and its application

A bronchitis and virus vaccine technology, which is applied in the directions of antiviral agents, viruses/phages, medical preparations containing active ingredients, etc. effect of disease

Active Publication Date: 2016-01-06
兆丰华生物科技(南京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by this invention is to screen out safe vaccines from clinically ill chickens for the problem that the existing branch vaccine strains are not suitable for new epidemic strains. A strain that is highly effective and can represent the current epidemic situation, and the preparation of vaccines with this strain can better prevent and control chicken infectious bronchitis

Method used

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  • Chicken infectious bronchitis virus vaccine strain (hf2 strain) and its application
  • Chicken infectious bronchitis virus vaccine strain (hf2 strain) and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Isolation and Identification of Infectious Bronchitis Virus HF2 Strain

[0020] 1. Virus isolation and culture

[0021] Aseptically collect the kidneys of dead chickens, add sterilized saline at a ratio of 1:5, grind, freeze and thaw 3 times, centrifuge at 5000rpm for 20min, filter and sterilize the supernatant, and inoculate 10 days-old SPF chicken embryos in the allantoic cavity eggs, 0.2ml per embryo, incubate chicken embryos at 37°C, irradiate embryos twice a day, discard dead embryos within 24 hours, take 5 embryos within 96 hours to harvest allantoic fluid, and continue to incubate until 144 hours, record chicken embryo lesions Happening. So blindly passed on for 5 generations. Harvested virus was stored at -80°C. The chicken embryos of the 3rd generation showed typical pathological changes caused by the virus, which manifested as growth retardation of chicken embryos, curling up of embryo bodies, and dehydration. To the fifth generation, the viru...

Embodiment 2I

[0043] The cultivation of embodiment 2 IBVHF2 attenuated strain

[0044] 1. Continuous subculture of chicken embryos weakened the virus strain

[0045] Use 9-11-day-old SPF chicken embryos for continuous passage to weaken the IBVHF2 strain, inoculate 5 chicken embryos through the allantoic cavity in each passage, 0.1ml / piece, incubate at 37°C, discard dead chicken embryos within 24 hours, 40 Two live embryos were harvested in about 1 hour, and the embryo fluid was collected aseptically for the next subculture, and the rest were kept for 144 hours to observe whether there were branched lesions in the chicken embryos. This was continuously passed down to the 100th generation. During a certain interval, virus content, sterility and mycoplasma detection, exogenous virus detection, and safety tests on susceptible chicks were carried out to determine whether the virulence was attenuated.

[0046] 2. Determination of virus content

[0047] The EID50 of the E5, E10, E30, E50, E70...

Embodiment 3I

[0063] Embodiment 3 IBVHF2 attenuated vaccine preparation

[0064] 1. Preparation of IBV virus liquid Dilute the above-mentioned HF2E80 as a seed poison 1000 times with sterilized normal saline, inoculate 100 10-day-old SPF chicken embryos in the allantoic cavity, 0.1ml per embryo, seal the pinhole, and continue to incubate at 37°C. There is no need to turn the eggs, and the eggs are illuminated once within 30 hours after inoculation, and the dead embryos are discarded. After that, the eggs are illuminated once every 4 to 6 hours, and the dead chicken embryos are taken out at any time until 40 hours. The chicken embryos are taken out, the air chamber is upright, and placed at 2-8°C to cool for 24 hours. Take out the cooled chicken embryo, sterilize the air cell, and then use aseptic surgery to peel off the egg shell of the air cell, remove the egg shell membrane, cut the chorioallantoic membrane and amniotic membrane (do not break the yolk), and absorb the chicken embryo flu...

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Abstract

The invention belongs to the field of veterinary biomedicine, and relates to a chicken infectious bronchitis vaccine strain HF2 and the application and application effect of the vaccine strain in preventing and controlling chicken infectious bronchitis. The microbial preservation number of the vaccine strain of the present invention is: 7708. The vaccine strain disclosed by the invention has good safety and good protective effect on chicken infectious bronchitis. The vaccine strain can be used to prepare inactivated single vaccine or combined vaccine for preventing and treating chicken infectious bronchitis, and the weakened virus strain can be used for preparing attenuated live vaccine or combined vaccine for preventing and treating chicken infectious bronchitis.

Description

Technical field [0001] The present invention is a new pharmaceutical technology field, involving a chicken infectious bronchitis virus vaccine and its application. Background technique [0002] AvianinFectiousbronchitis is a kind of acute altitude infectious disease caused by chicken infectious bronchitis virus virus (AvianinFectiousBronchitisvirus). The disease has a wide range of diseases, and there is a place where chickens have the disease.Chickens of all varieties and age can be infected. In addition to causing bronchitis to show respiratory symptoms such as cough, sneezing, and bronchial tubal Luo Yin, egg -producing hens will cause decreased egg production and decrease in egg quality. Some strains will cause kidneyDiseases cause death in severe cases.The decline in poultry production indicators caused by IBV infection and vaccines and other human and material resources for preventing and controlling virus infections have caused huge economic losses and are one of the most ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/215A61P31/14C12R1/93
Inventor 许秀梅毛火云王伟
Owner 兆丰华生物科技(南京)有限公司
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