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Preparation method of high-purity lycopene

A lycopene, high-purity technology, applied in the field of high-purity lycopene preparation, can solve the problems of low product purity, difficult production control, low yield, etc.

Inactive Publication Date: 2013-12-18
NANJING TONGZE AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the patent CN1298904A discloses a method for producing lycopene with a purity of 10% from tomato paste. Lycopene is obtained by adding water and stirring, centrifugal dehydration, alkali washing, water washing, drying and crushing, heating extraction, flash evaporation, and crystallization. The shortcoming is that the product purity is not high, the operation is very complicated, and the production is not easy to control; CN1334328A has announced a kind of preparation method that adopts supercritical fluid to extract lycopene oleoresin, that is, after the tomato is pretreated, directly use CO 2 Or the supercritical liquid of low-molecular-weight hydrocarbon extracts raw material and obtains lycopene oleoresin, and its shortcoming is that cost is high, and output is low; The method of purity lycopene, the product purity that this method obtains can reach more than 95%, but has used multiple organic solvents, comprises highly toxic acetonitrile, chloroform etc., simultaneously production efficiency is low, cost is high, and separation scale is limited, Not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take fresh tomatoes, peel and remove seeds, and make a slurry; add 7 times of n-hexane to the above slurry, and then add 0.08% of the tomato slurry weight of the compound enzyme of cellulase and pectinase, and control the temperature at 45°C , stirred, and the enzymolysis time was 4 hours; the material after enzymatic conversion was placed in an ultrasonic extractor, the ultrasonic power was 80W, and the extraction time was 30 minutes; the extract was centrifuged, the supernatant was taken out, and an appropriate amount of hydrogen was added to it Potassium solution, cooled to room temperature after saponification, transferred to a separatory funnel; added distilled water and shaken evenly, allowed to stand, layered, released the water layer until the upper liquid was neutral, and recovered the water layer with an organic solvent each time Lycopene, combined with the upper layer, the combined extract with an appropriate amount of anhydrous NaSO 4 Dry and filter with suc...

Embodiment 2

[0023] Take fresh tomatoes, peel and remove seeds, and make a slurry; add 6 times of ethyl acetate to the above slurry, and then add 0.06% of the weight of the tomato slurry with a compound enzyme of cellulase and protease, and control the temperature at 50°C. Stir, enzymatic hydrolysis time is 3.5h; put the material after enzymatic conversion in an ultrasonic extractor, the ultrasonic power is 60W, and the extraction time is 25min; centrifuge the extract, take out the supernatant, and add an appropriate amount of hydrogen to it Sodium solution, after saponification, cool to room temperature, transfer to a separatory funnel; add distilled water and shake well, let stand, separate layers, release the water layer until the upper liquid is neutral, and recover each water layer with ethyl acetate The lycopene, combined with the upper layer, the combined extract with an appropriate amount of anhydrous NaSO 4 Dry and filter with suction to obtain the filtrate; the filtrate is remove...

Embodiment 3

[0025] Take fresh tomatoes, peel and remove seeds, and make a slurry; add 5 times of petroleum ether to the above slurry, and then add a compound enzyme of cellulase and pectinase at 0.07% of the weight of the tomato slurry, and control the temperature at 40°C , stirred, and the enzymolysis time was 3 hours; the material after enzymatic conversion was placed in an ultrasonic extractor, the ultrasonic power was 100W, and the extraction time was 20 minutes; the extract was centrifuged, the supernatant was taken out, and an appropriate amount of potassium carbonate was added to it Solution, after saponification, cool to room temperature, transfer to a separatory funnel; add distilled water and shake well, let stand, separate layers, release the water layer until the upper liquid is neutral, and recover the tomato in each water layer with petroleum ether Red pigment, combined with the upper layer, the combined extract with an appropriate amount of anhydrous NaSO 4 Drying and sucti...

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Abstract

The invention relates to a preparation method of high-purity lycopene, which comprises the following steps: (a) removing the peel and seeds of tomatoes, and pulping; (b) performing enzymolysis by use of a bio-enzyme; (c) performing ultrasonic-assisted extraction; (d) performing saponification and impurity removal; (e) performing extraction and crystallization. According to the method provided by the invention, the tomatoes are used as raw materials, the overlay effect of the enzymolysis and ultrasonic is fully used, the lycopene in the tomatoes is dissolved out to the greatest degree, and the high-purity lycopene is obtained through a series of purification treatment. The method provided by the invention has the advantages of simple process, short extraction time, high extraction rate and high purity, and is environment-friendly and easy for realizing industrial production.

Description

technical field [0001] The invention relates to a preparation method of high-purity lycopene, in particular to a method for extracting lycopene by enzymatic method-ultrasonic wave. Background technique [0002] Lycopene, a linear hydrocarbon compound composed of 11 conjugated double bonds and 2 non-conjugated double bonds in the molecular structure, is a type of carotenoid without oxygen. It has anti-cancer and anti-cancer effects, and is of great significance for preventing various adult diseases such as cardiovascular disease and arteriosclerosis, enhancing the human immune system, and delaying aging. [0003] Due to the unique functions of lycopene, health products with lycopene have been on the market since 1994, and the output value has been increasing continuously, and breakthroughs have been made in clinical trials. The prospect of using lycopene as health products is very promising. Some people predict that it It may develop into three types of medicines. Therefore,...

Claims

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Application Information

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IPC IPC(8): C07C11/21C07C7/00
Inventor 杨存
Owner NANJING TONGZE AGRI SCI & TECH
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