Preparation method of barley extract fermented by lactobacillus and anti-tumor effect of barley extract fermented by lactobacillus
A technology of lactic acid bacteria fermentation and lactic acid bacteria, which is applied in the direction of anti-tumor drugs, food preparation, medical preparations containing active ingredients, etc., can solve the problems that anti-tumor extracts have not been found in patents or literature reports, etc., to achieve rich nutrition and functions, Ease of quality control and low production cost
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Embodiment 1
[0035] Weigh 20.0g of barley flour crushed through a 60-mesh sieve into a 500mL Erlenmeyer flask, add 1:6 ratio of water to it and mix evenly, first hydrolyze it with 0.015% β-glucanase at 40°C for 30 min, and then use 4 % Of Lactobacillus plantarum ( Lactobacillus plantarum Dy-1) Fermented at 30°C for 48h. After the fermentation is completed, FBE is centrifuged at 6000rpm / min for 20min at room temperature, the supernatant is collected, and freeze-dried for 24h to obtain FBE.
[0036] Weigh 0.5g of FBE dissolved in 50ml of deionized water, pass a 0.22um filter membrane, and adjust the filtrate to 0.125 mg / mL, 0.25 mg / mL, 0.5 mg / mL, 1.0 mg / mL, 2.0 mg / ml, 4.0 mg / ml was added to HT-29 tumor cells, and the cells were cultured for 24h, 48h, 72h, and the inhibition rate of FBE on colon cancer HT-29 cells was determined by MTT method. The results showed that the concentration of the extract was positively correlated with the inhibition rate of tumor cell proliferation, and the maximum...
Embodiment 2
[0038] The FBE prepared above was dissolved in physiological saline to prepare the concentrations of 0.2 g / ml and 0.1 g / ml, and was treated with a sterile membrane for use. Human HT-29 colon cancer cells were inoculated subcutaneously into the abdomen of BALB / c nude mice aged 4-6 weeks, and 2g / kg / d, 1g / kg / d, 2g / kg / d+5-FU ( 25 mg / kg / d, ip, 7 days) of FBE was administered to tumor-bearing nude mice by gavage for 30 consecutive days. At the same time, the same dose of normal saline group and intraperitoneal injection (25 mg / kg / d, 7 days) compared with the 5-FU group. The anti-tumor effect of FBE was confirmed by the volume of the transplanted tumor measured with a vernier caliper and the final tumor inhibition rate ( figure 2 ,3). The results showed that the tumor inhibition rate of the 2g / kg / d+5-FU mixed dose group could reach 53.36%.
[0039] Determination of superoxide dismutase (SOD) in the liver of tumor-bearing nude mice with a kit ( Figure 5 ), glutathione-peroxidase (GSH-...
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