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Method for improving adenosine fermentation output through feeding hypoxanthine

A technology of hypoxanthine and adenosine, which is applied in the field of adenosine production by adding precursors in the middle, can solve the problems of limited popularization and application, high cost of adenosine, and low adenosine output, and can prolong production time and yield The effect of maintaining stability and simple production equipment

Inactive Publication Date: 2013-11-27
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of high cost, serious pollution, limited popularization and application of adenosine produced by the existing chemical method and enzymatic method, and the low yield of adenosine produced by fermentation method, and to provide a method for increasing adenosine production by feeding precursors. Method of Fermentation Yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The formula of slant medium is in g / L: glucose 2, peptone 10, beef extract 10, yeast powder 5, NaCl 2.5, agar 30, pH 7.0.

[0027] Seed medium formula in g / L: oral glucose 20, corn steep liquor 25 mL / L, soybean meal hydrolyzate 10 mL / L, yeast powder 5, monosodium glutamate 5, peptone 8, KH 2 PO 4 1. MgSO 4 ·7H 2 O 0.4, Urea 5, Xanthine 0.03, Histidine 0.03, pH 7.2.

[0028] Fermentation medium in g / L: oral glucose 100, yeast extract 18, monosodium glutamate 12, K 2 HPO 4 3. Xanthine 0.03, L-histidine 0.03, MgSO 4 ·7H 2 O5, MnSO 4 0.006, FeSO 4 ·7H 2 O 0.006, CaCl 2 2. Corn steep liquor 15 mL / L, soybean meal hydrolyzate 10 mL / L.

[0029] Bacillus subtilis XGL was used as the starting strain, inoculated to the activated slant, cultured at 30 °C for 22 h, scraped the slant strain with an inoculation loop and inoculated into the seed medium, cultivated at 31 °C, 200 rpm for 10.5 h; at 10% (v / The inoculation amount of v) was transferred to a 5 L fermenter wi...

Embodiment 2

[0032] The slant medium formula is in g / L: glucose 1, peptone 5, beef extract 5, yeast powder 2, NaCl 1, agar 20, pH 7.0.

[0033] Seed medium formula in g / L: oral glucose 10, corn steep liquor 15 mL / L, soybean meal hydrolyzate 5 mL / L, yeast powder 5, monosodium glutamate 3, peptone 5, KH 2 PO 4 0.5, MgSO 4 ·7H 2 O 0.2, Urea 2, Xanthine 0.01, Histidine 0.01, pH 7.2.

[0034] Fermentation medium in g / L: oral glucose 80, yeast extract 12, monosodium glutamate 8, K 2 HPO 4 1, Xanthine 0.01, L-histidine 0.01, MgSO 4 ·7H2 O 1,MnSO 4 0.003, FeSO 4 ·7H 2 O 0.003, CaCl 2 1. Corn steep liquor 10 mL / L, soybean meal hydrolyzate 10 mL / L.

[0035] Bacillus subtilis XGL was used as the starting strain, inoculated to the activated slant, cultured at 28°C for 20 h, scraped the slant with an inoculation loop and inoculated into the seed medium, cultivated at 28°C, 180 rpm for 8 h; at 10% (v / The inoculation amount of v) was transferred to a 5 L fermenter with fermentation medium...

Embodiment 3

[0038] The formula of slant medium is in g / L: glucose 3, peptone 10, beef extract 10, yeast powder 5, NaCl 2.5, agar 30, pH 7.2.

[0039] Seed medium formula in g / L: oral glucose 20, corn steep liquor 25 mL / L, soybean meal hydrolyzate 10 mL / L, yeast powder 10, monosodium glutamate 8, peptone 10, KH 2 PO 4 1.5, MgSO 4 ·7H 2 O 0.8, Urea 7, Xanthine 0.05, Histidine 0.05, pH 7.2.

[0040] Fermentation medium in g / L: oral glucose 120, yeast extract 18, monosodium glutamate 12, K 2 HPO 4 3. Xanthine 0.05, L-histidine 0.05, MgSO 4 ·7H 2 O5, MnSO 4 0.006, FeSO 4 ·7H 2 O 0.006, CaCl 2 3. Corn steep liquor 20 mL / L, soybean meal hydrolyzate 30 mL / L.

[0041] Bacillus subtilis XGL was used as the starting strain, inoculated to the activated slant, cultured at 32°C for 24 h, scraped the slant with an inoculation loop and inoculated to the seed medium, cultivated at 33°C, 220 rpm for 11 h; at 10% (v / The inoculation amount of v) was transferred to a 5 L fermenter with fermen...

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Abstract

The invention provides a method for improving the adenosine fermentation output through feeding hypoxanthine. The method which treats Bacillus subtilis XGL as a producing strain is characterized in that a precursor is intermittently fed at a uniform speed 6-12 times after the strains grow in a logarithmic phase. The utilization of the method to carry out the adenosine fermentation production makes the precursor in a metabolism flow for the adenosine synthesis sufficient, so the adenosine output is improved to 33.5g / L, and is 28.8% higher than the output obtained through other methods, and the fermentation period is shortened to 50h. The method has the advantages of adenosine output increase, and simple and repeatable operation.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and fermentation engineering, and in particular relates to a method for fermenting and producing adenosine by adding precursors in the middle. Background technique [0002] Adenosine (Adenosine), that is, adenosine nucleoside, the molecular formula is C 10 h 13 N 5 o 4 , the molecular weight is 267.24, and the chemical name is 9-β-D-furan ribose 9-β-D-ribofuranosyl adenine. As an intermediate product of myocardial energy metabolism, adenosine plays an important pathophysiological role in maintaining the homeostasis of the cardiovascular system and various injury mechanisms. Adenosine is the first-line drug approved by the FDA for the treatment of paroxysmal supraventricular tachycardia (PSVT) in the United States, and has become a routine drug for emergency treatment of tachyarrhythmias and drug stress tests. [0003] With the continuous expansion of the scope of action of adenosine,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/40C12R1/125
Inventor 陈宁刘玥何菊华徐庆阳谢希贤张成林刘淑云
Owner TIANJIN UNIV OF SCI & TECH
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