Epidemic encephalitis B/forest encephalitis hybrid virus and application of virus
A technology of forest encephalitis virus and forest encephalitis, applied in the field of genetic engineering, achieves good application prospects, high safety, and stable attenuation characteristics
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[0041] Embodiment 1, constructing the Japanese encephalitis / forest encephalitis chimeric virus with the attenuated strain of Japanese encephalitis virus as the gene backbone
[0042] In this example, the attenuated JE virus SA14-14-2 strain is used as the gene backbone to construct the JE / forest encephalitis chimeric virus, wherein the cDNA sequence corresponding to the genomic RNA of the JE virus attenuated strain SA14-14-2 As shown in sequence 4 in the sequence listing, the 1st-95th position from the 5' end is 5'UTR; the 96th-476th position is the coding gene of capsid protein C (C); the 477th-2468th position is the deletion of E The gene encoding the prM-E protein with 3 amino acid residues at the carboxy-terminal of the protein (prM-EΔ3); the 2469-2477th position is the 3-amino acid residue encoding gene at the carboxy-terminal end of the E protein (E3); the 2478th-10394 The position is the coding gene (NS) of the non-structural protein NS; the 10395-10977 position is the ...
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