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Stable nanoscale superparamagnetic iron oxide solution as well as preparation method and application thereof

A superparamagnetic iron oxide, nano-scale technology, applied in the fields of medicine and chemistry, can solve problems such as unsatisfactory effect, inconvenient use, large dose, etc., and achieve the effects of stable nano-solution characteristics, improved imaging performance, and good dispersion.

Inactive Publication Date: 2013-09-25
桑迪(武汉)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, based on the unsatisfactory effect, large dose, inconvenient use and potential side effects of excessive iron in the body, Feridex has been withdrawn from the market

Method used

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  • Stable nanoscale superparamagnetic iron oxide solution as well as preparation method and application thereof
  • Stable nanoscale superparamagnetic iron oxide solution as well as preparation method and application thereof
  • Stable nanoscale superparamagnetic iron oxide solution as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Preparation method of nanoscale superparamagnetic iron oxide solution

[0053] Weigh 0.71 g of iron acetylacetonate, 2.02 g of 1,2-dodecanediol, 1.83 g of oleic acid, 1.68 g of oleylamine, and 20.14 g of diphenyl ether, mix well, stir and heat to 200 degrees Celsius in a nitrogen environment, Constant temperature for 30 minutes, then heated to 220 degrees Celsius, after constant temperature for 30 minutes, stop heating, and cool to room temperature. Add 45 milliliters of ethanol to the reaction solution, stir, precipitate, centrifuge at 5000 g for 10 minutes, discard the supernatant, and collect the precipitate; In the process, after removing the residual solid, the n-hexane was rotated and evaporated to obtain superparamagnetic iron oxide nanoparticle solid particles;

[0054] Take the above-mentioned superparamagnetic iron oxide nanoparticles, citric acid, and N,N-dimethylformamide to prepare 40 ml of solution, stir and heat to 80 degrees Celsius under ni...

Embodiment 2

[0058] Example 2 Nanoscale Superparamagnetic Iron Oxide Intravenous Injection

[0059] The stable nano-scale superparamagnetic iron oxide solution prepared above was diluted with phosphate buffer to a superparamagnetic iron oxide solution containing 2 mg iron per mL of solution, and then filter-sterilized with a 0.22-micron sterile filter and used as an intravenous injection. The recommended injection dose is 0.05 ml / kg.

[0060]

Embodiment 3

[0061] Example 3 Determination of Magnetic Resonance Imaging in Liver Tumor Models in Situ

[0062] In the animal model, mice with early orthotopic liver cancer tumors were first scanned and analyzed by T1 and T2 images in a magnetic resonance MRI instrument, and then intravenously injected with the nano-scale superparamagnetic iron oxide solution provided by the present invention , the injection amount is the solution dose containing 25 micrograms of iron, and then T1 and T2 image scanning and imaging analysis are carried out in the magnetic resonance MRI instrument, and the results show that the tumor has magnetic resonance under the nano-scale superparamagnetic iron oxide solution provided by the present invention. Imaging performance in contrast has been significantly improved, clearly showing the location, border and size of the tumor, and the tumor imaging effect is still significant after 24 hours; while the early tumor living model without contrast agent has blurred t...

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Abstract

The invention provides a stable nanoscale superparamagnetic iron oxide solution which is used as a contrast agent for magnetic resonance imaging (MRI). The superparamagnetic iron oxide solution is characterized in that the particle size of nanoscale superparamagnetic iron oxide particles is stabilized to between 60 and 75 nanometers when the superparamagnetic iron oxide solution is stored for 12 months at an environmental temperature of 4 to 38 DEG C, and the nanoscale superparamagnetic iron oxide particles are in high dispersibility and free from agglomerating or precipitating. After nanoscale superparamagnetic iron oxide solution containing 25mcg of iron, provided by the invention, is subjected to intravenous injection in the MRI of an early-stage in-situ hepatic cancer tumor live model of a rat, the imaging performance of the tumor in the MRI is obviously improved, the position, boundary and size of the tumor can be clearly displayed, and the tumor image effect remains significant after 24 hours; the phenomenon that the boundary and size of the tumor are unclear occurs in the MRI of the early-stage in-situ hepatic cancer tumor live model without contrast agent. Therefore, the nanoscale superparamagnetic iron oxide solution provided by the invention has a stable nanometer solution characteristic, is obvious in tumor impacting effect under low-dosage injection, and unique in innovation and application prospect.

Description

technical field [0001] The invention belongs to the fields of medicine and chemistry, and relates to a magnetic resonance imaging (MRI) contrast agent, in particular to a preparation method of a novel and stable nano-scale superparamagnetic iron oxide solution and its application in medical diagnosis, especially as an image contrast agent in Applications in tumor diagnosis. Background technique [0002] The superparamagnetic iron oxide solution contrast agent is an intravenous injection agent for improving the imaging diagnosis effect of liver cancer in clinical tumor diagnosis. Liver cancer is a major disease in my country, with about 350,000 newly discovered cases each year, accounting for about 50% of new cases in the world, and 300,000 deaths due to liver cancer every year, and its fatality rate ranks second in my country. The main reason for the high mortality rate of liver cancer is that most of the patients with liver cancer in my country are already in the middle an...

Claims

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Application Information

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IPC IPC(8): A61K49/06
Inventor 周琦冰金伟魏裕双
Owner 桑迪(武汉)生物科技有限公司
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