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Method for establishing animal model of Alzheimer's disease

An Alzheimer's disease, animal model technology, applied in the field of biology, can solve the problems of the inability to clarify the dose-response relationship of PF-MF interaction, the inability to fully clarify the mechanism of action, and the lack of comparability of research results.

Inactive Publication Date: 2013-09-25
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, due to the different experimental conditions such as magnetic field exposure parameters and research objects, the research results on the biological effects of PF-MF are not comparable, and even caused some controversy. As a result, it is still unclear how PF-MF interacts with organisms dose-effect relationship, and its mechanism of action cannot be fully elucidated

Method used

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  • Method for establishing animal model of Alzheimer's disease
  • Method for establishing animal model of Alzheimer's disease
  • Method for establishing animal model of Alzheimer's disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1Morris water maze study

[0033]Eighty 8-week-old secondary male Wistar rats weighing 212.6±13.6 grams (provided by the Experimental Animal Center of the Academy of Military Medical Sciences) were studied in the Morris water maze, and the Morris water maze detection and analysis system (SLY-WMS) was provided by Beijing Shuo Developed by Linyuan Technology Company. During the test, the room temperature is controlled at 25 degrees Celsius, and it is kept quiet and low-light; add clear water to the pool, adjust the water temperature to 25 degrees Celsius, and make the water surface 1 cm below the platform; the water surface is divided into I, II, III, IV, and 4 clockwise. four quadrants, the platform is placed in the center of quadrant I; the rats enter the water from the midpoint of the pool wall of quadrant I to IV in turn; record the time that the rat finds and stays on the platform within 60 seconds, if the platform is not found for more than 60 seconds, it...

Embodiment 2

[0034] Example 2 Power Frequency Magnetic Field Exposure

[0035] By adjusting the voltage regulator of the power frequency magnetic field exposure device, the voltage of the power frequency magnetic field exposure device is 172.5 volts and the input current is 1.65 ampere. Tested by a Gauss meter (EFA300, Narda Safety Test Solutions), at this time, a uniform power frequency magnetic field is generated between the two sub-coils, the magnetic field strength at the center point is 400μT, and the frequency is 50Hz. Rats in the ELF group and the AD+ELF group were kept in the animal breeding box of the animal power frequency magnetic field exposure device, and the exposure continued for 60 days. The Con group and the AD group were placed in the same size animal breeding box and raised for 60 days in an environment with a magnetic field strength lower than 400nT. During the experiment, the room temperature was kept constant at 22 degrees Celsius, and the difference between the inte...

Embodiment 3D

[0037] Example 3D-galactose intraperitoneal injection

[0038] On the 1st to 42nd days when the rats were kept in the above animal power frequency magnetic field exposure device, the rats in the AD+ELF group and the AD group were intraperitoneally injected with D-galactose diluted with sterile saline (Sigma, USA) , the dose was 50mg / kg / d; at the same time, ELF group and Con group rats were intraperitoneally injected with equal volume of sterile saline.

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Abstract

The invention provides a method for establishing an animal model of an Alzheimer's disease. The method includes feeding a rat for at least 60 days under the condition of exposing to a power-frequency magnetic field; feeding the rat with drug D of galactose and beta of amyloid protein liquid during the process of feeding, and accordingly obtaining the animal model of the Alzheimer's disease. Thus, the method can be a useful supplement of a single-factor-induced animal model of the Alzheimer's disease. According to an embodiment of the method, characteristic pathological changes of the animal of the Alzheimer's disease can be expressed; the method has a characteristic of learning and memory impairment, is consistent with the environmental requirements of long-time and lasting exposure to the power-frequency magnetic field, and has the advantages of stable performance, good reproducibility, and low cost.

Description

technical field [0001] The invention relates to the field of biology, in particular, the invention relates to a method for establishing an animal model of Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD) is a degenerative neuropathy mainly characterized by progressive cognitive impairment and memory decline. Its characteristic pathological changes are: senile plaques formed by the aggregation of β-amyloid (Aβ) in the brain, neurofibrillary tangles formed by abnormal aggregation of tau protein in neurons, different degrees of apoptosis or loss of neurons, and Astrogliosis and hypertrophy. AD not only has a devastating impact on the patient himself and his family, but also has the potential to become a global crisis that endangers public health and social health systems as the aging population surges in various countries. [0003] Power Frequency Magnetic Field (PF-MF) refers to the electromagnetic field generated by power facilities such as high-v...

Claims

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Application Information

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IPC IPC(8): A01K67/02
Inventor 左红艳刘肖王德文宋涛徐新萍李杨王少霞王水明彭瑞云高亚兵吕琰
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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