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Fluid nutrient medium for large-scale cultivation of termitomyces albuminosus mycelium and application thereof

A technology of liquid culture medium and Gallus fir fungus, applied in application, fertilizer mixture, fertilization device, etc., can solve problems such as lack of understanding, and achieve the effect of promoting utilization and cost saving

Inactive Publication Date: 2013-06-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although people have done a lot of research on the artificial cultivation of Termitomyces albuminosus, it is known that the cultivation of Termitomyces albuminosus requires a certain amount of CO 2 Concentration, high humidity and acidic conditions, but so far, due to the lack of understanding of the role of plant species composition, metal ion species and concentration, acid species and concentration, symbiotic bacteria in the fungus garden, etc. Also do not have a kind of method that utilizes lignocellulose as main nutrient medium large-scale cultivation mycelium of gallinaceous fungus

Method used

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  • Fluid nutrient medium for large-scale cultivation of termitomyces albuminosus mycelium and application thereof
  • Fluid nutrient medium for large-scale cultivation of termitomyces albuminosus mycelium and application thereof
  • Fluid nutrient medium for large-scale cultivation of termitomyces albuminosus mycelium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Strains:

[0029] Dig the black-winged soil termite nest from the wild, and bring the fungus garden back indoors. On the same day, transfer the small white coccus (the vegetative stage of Gallus fructus) to the slant medium (potato dextrose agar medium), select a well-growing slant for pipette purification, and keep the purified strain as the mother species;

[0030] Preparation of potato dextrose agar medium: 200g of peeled potatoes, 20g of glucose, 15-20g of agar, 1000ml of distilled water. Cut peeled potatoes into small pieces, boil in water for 30 minutes, filter with 8 layers of gauze, add glucose and agar to the filtrate, dilute to 1000ml with water, autoclave it and pour it into a glass tube for strain cultivation and purification.

[0031] Acquisition of the bacterium liquid of Gallinarum gallinarum: Add the potato dextrose agar medium and the mycelia cultured on the potato dextrose agar into the sterile water according to the solid-to-liquid mass ratio of 1:1,...

Embodiment 2

[0045] The quality formula of the culture medium is as follows, and others are the same as in Example 1:

[0046]

[0047]

[0048] Using this formula to cultivate, the amount of mycelium obtained is increased by about 3800 times.

Embodiment 3

[0050] The quality formula of the culture medium is as follows, and others are the same as in Example 1:

[0051]

[0052]

[0053] Using this formula to cultivate, the amount of mycelium obtained is increased by about 3000 times.

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Abstract

The invention provides fluid nutrient medium for large-scale cultivation of termitomyces albuminosus mycelium and application thereof. The culture medium is prepared from the following materials in parts by weight: 50-70 parts of lignocellulose powder, 30-50 parts of water, 0.0001-0.1 part of monopotassium phosphate, 0.0001-0.1 part of sodium chloride, 0.0001-0.1 part of calcium chloride, 0.0001-0.1 part of cobalt chloride, 0.0001-0.1 part of magnesium sulfate, 0.0001-0.1 part of manganese sulfate, 0.0001-0.1 part of ferrous sulfate, 0.0001-0.1 part of copper sulfate, 0.0001-0.1 part of zinc sulfate, 0.0001-0.1 part of aluminum potassium sulfate, 0.0001-0.1 part of ammonium tartrate, 0.0001-0.1 part of boric acid, 0.0001-0.1 part of citric acid, 0.0001-0.1 part of formic acid, 0.0001-0.1 part of acetic acid, 0.0001-0.1 part of fumaric acid, 0.0001-0.1 part of succinic acid, 0.0001-0.1 part of propylene-1,2,3-tricarboxylic acid, and 0.0001-0.1 part of guaiacol. The termitomyces albuminosus of the fluid nutrient medium disclosed by the invention is customized, and can be used for large-scale indoor cultivation of termitomyces albuminosus mycelium by using lignocelluloses; and the fluid nutrient medium is low in cost and high in yield.

Description

(1) Technical field [0001] The invention relates to a liquid culture medium for large-scale cultivation of the mycelia of the collybia fruticosa and application thereof. (2) Background technology [0002] Termitomyces albuminosus belongs to the Basidiomycetes Phylomycetes Agaricaceae Agaricaceae and is a rare and precious wild edible fungus. People have more than 200 years of scientific research on the Gallus fir fungus. In 1799, the German natural scientist J.D.Kǒning first discovered many fungi that could form small white balls on the fungus beds in the termite nests in eastern India. That is, the fungus that was later called the gallium fir. For the termites, the mycelium of the Gallium fruticosa is their main food, and it is for this purpose that they cultivate or "cultivate" the mycelium of the Gallium fruticosa. The mycelium of the gallium fruticosa is widely distributed throughout the nest, and when the termites go to feed, it is replaced by further growth. Termite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
Inventor 莫建初
Owner ZHEJIANG UNIV
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