Chinese herb extract feed additive capable of effectively preventing and treating porcine epizootic diarrhea
A porcine epidemic diarrhea and Chinese herbal medicine extract technology, applied in the field of Chinese herbal medicine extract feed additives, can solve the problems of difficult control of quality indicators, affecting the taste of feed, single drug resistance, etc., to improve feed utilization efficiency, enhance body immunity, treat short time effect
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[0035] Preparation of Bacillus subtilis bacterial agent:
[0036] (1) Primary seed cultivation: Inoculate the slant strain of Bacillus subtilis into a 500-mL shaker flask with a medium capacity of 100 mL, a rotary shaker at 180 rpm, a cultivation temperature of 30°C, and a cultivation time of 24 hours;
[0037] (2) Secondary seed cultivation: Put the first-grade seeds into 500 ml second-grade seed shake flasks according to the inoculation amount of 10%, and the cultivation conditions are the same as the first-grade seeds;
[0038] (3) Tertiary seed cultivation: Inoculate the secondary seeds with 10% inoculum amount into a 5000 ml tertiary seed shaker flask, with a medium capacity of 1000 ml, a rotary shaker at 100 rpm, and a culture temperature of 30°C. Time 24 hours;
[0039] (4) First-level seed tank cultivation: put the third-level seeds into the first-level seed tank with a total volume of 150L with 10% inoculation amount, the fermentation medium capacity is 100L, the cul...
Embodiment 1
[0066] A Chinese herbal medicine extract feed additive capable of effectively preventing and treating porcine epidemic diarrhea consists of the following raw materials in parts by weight:
[0067] Centella asiatica powder 10 parts; turmeric powder 5 parts; cassia seed powder 8 parts; burdock seed powder 10 parts; 5 parts of bacterial agent.
[0068] The preparation method of described Chinese herbal medicine preparation is as follows:
[0069] Accurately weigh 20 parts of Centella asiatica; 10 parts of cassia seed; 5 parts of arctium; Keep the temperature at 70°C for 2 hours, add a mixture of ethanol and propanol twice the weight of the mixed material, control the temperature to 60°C, keep it for 3 hours, and filter; the filtrate is concentrated in vacuum and then freeze-dried to obtain a Chinese herbal medicine preparation.
[0070] The mass ratio of ethanol and propanol is 1:1; the ethanol concentration is 95%; the propanol concentration is 100%;
[0071] The preparation ...
Embodiment 2
[0078] A Chinese herbal medicine extract feed additive capable of effectively preventing and treating porcine epidemic diarrhea consists of the following raw materials in parts by weight:
[0079] 15 parts of centella asiatica powder; 10 parts of turmeric powder; 15 parts of cassia seed powder; 20 parts of burdock seed powder; 10 parts of bacterial agent.
[0080] The preparation method of described Chinese herbal medicine preparation is as follows:
[0081] Accurately weigh 25 parts of Centella Asiatica; 18 parts of Semen Cassiae; 15 parts of Arctium; Keep the temperature at 90°C for 4 hours, add a mixture of ethanol and propanol 3 times the weight of the mixed material, control the temperature to 78°C, keep it for 4 hours, filter; the filtrate is concentrated in vacuum and then freeze-dried to obtain a Chinese herbal medicine preparation.
[0082] The mass ratio of described ethanol and propanol is 1:1.5, and ethanol concentration is 95%; Propanol concentration is 100%;
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