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Preparation and application of a strain of Chaetomium globosa and its metabolite aflatoxin

A technology of aspergillus flavus and Chaetomium globosa, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of unreported antioxidant activity, etc., and achieve the effect of strong antioxidant activity

Inactive Publication Date: 2014-10-08
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chaetomium globosum CDW7 involved in this paper is a plant endophyte isolated from healthy Ginkgo biloba leaves, and its secondary metabolites, flavipin (1,2-benzonedicarboxaldehyde-3,4,5-trihydroxy-6-methyl ) was first isolated from Aspergillus flavipes and Aspergillus terreus by Raistrick. It has certain antibacterial and nematicide activity, but its antioxidant activity has not been reported so far.

Method used

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  • Preparation and application of a strain of Chaetomium globosa and its metabolite aflatoxin
  • Preparation and application of a strain of Chaetomium globosa and its metabolite aflatoxin
  • Preparation and application of a strain of Chaetomium globosa and its metabolite aflatoxin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1. Isolation and purification of endophytic strain CDW7:

[0019] Collect fresh and healthy ginkgo leaves, wash and soak in 75% alcohol for 30 seconds, rinse with sterile water for 3 times, then soak in 3-5% NaClO solution for 3-5 minutes, and rinse with sterile water for 3-5 times. Under sterile conditions, cut the surface-sterilized ginkgo leaves into small pieces of 0.5×0.5cm, and place them on PDA for culture at a constant temperature of 25°C. After the bacteria grow out, pick the mycelium at the edge of the colony for purification and culture Until the colonies have the same shape, they are stored on a PDA slant to obtain endophytic bacteria of Ginkgo biloba. One of the strains is numbered CDW7, which is now preserved in the China General Microorganism Culture Collection Center (CGMCC for short), with a preservation date of October 15, 2012 and a preservation number of CGMCC No.6658.

Embodiment 2

[0020] Embodiment 2. Identification of endophytic strain CDW7:

[0021] The endophytic fungus CDW7 is on the PDA plane medium, and the initial stage of the colony is white (see attached figure 1 ), fades to dark cyan (see attached figure 2 ), brown on the back, with irregular edges. Ascothecia are scattered or grouped, ovate or nearly spherical, size (418-610) μm×(222-277) μm (see attached image 3 ); Ascus shell wall is composed of dark brown interlaced filamentous tissue, apical hairs without septa, brown, zigzag or helically curled, with fine spines on the surface, base diameter is 3.1 μm; ascospores are brown, subspherical or lemon-shaped, Size (7.5-10.2) μm × (6.5-7.5) μm, with a terminal bud hole (see attached Figure 4 ). The above morphological characteristics of CDW7 are consistent with the morphological characteristics of Chaetomium sp. fungi.

[0022] The mycelia of the activated CDW7 strain were picked into the PDB medium, cultured at 25°C for 5 days, and the...

Embodiment 3

[0024] Embodiment three. The preparation of compound aflatoxin (flavipin):

[0025] (1) Fermentation culture of CDW7: inoculate the strain of Chaetomium globosa CDW7 stored at 4°C on a PDA plate, culture it in a constant temperature incubator at 25°C for 5 days, and take 12 bacteria cakes with a diameter of 0.5cm on the plate The block was put into a sterilized 250mL Erlenmeyer flask filled with 100mL PDB medium, and cultured at 25°C and 150rpm for 14 days.

[0026] (2) Obtaining of the crude extract of the fermented product: take the fermented liquid obtained in the above step (1), separate the thalline and the fermented liquid with gauze, and obtain 2 L of the total fermented filtrate, and extract the fermented filtrate with dichloromethane solvent for 3-5 times, the combined extracts were concentrated under reduced pressure to obtain the fermented crude extract F1 (2g).

[0027] (3) Separation and purification of the compound: the crude extract of the fermented product obt...

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Abstract

The invention provides preparation and application of chaetomium globosum and metabolite flavipin thereof. The strain is endophytic fungi obtained by separating gingko leaves, is identified to be chaetomium globosum with a number of CDW7, and is preserved in the China General Microbiological Culture Collection Center with the register number of CGMCC No. 6658. The invention relates to preparation and application of CDW7 metabolite flavipin at the same time. The preparation method comprises the step that the organic solvent extract of the strain CDW7 fermentation broth is subjected to gel column chromatography and recrystallization. The flavipin has strong anti-oxidation activity, and can be used as an antioxidant for application.

Description

technical field [0001] The invention relates to the preparation and application of the chaetomium fungus and its metabolite aflatoxin. Background technique [0002] More and more free radical biology and free radical medical research results show that the occurrence and development of cardiovascular diseases, tumors, senile dementia, aging, diabetes, AIDS and other diseases are closely related to active oxygen and free radicals, looking for and The development of new, efficient and safe natural antioxidants is one of the important topics in the research of prevention and treatment of major diseases closely related to active oxygen and free radicals. [0003] Plant endophytes refer to those fungi or bacteria that live in various tissues and organs of healthy plants during certain stages or all stages of their life history. The infected host plants (at least temporarily) do not show external symptoms and can Endophysis was demonstrated by histological isolation of strictly su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P7/00A61K31/11A61P39/06C12R1/645
Inventor 叶永浩肖玉李红霞李枞刘俊彦
Owner NANJING AGRICULTURAL UNIVERSITY
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