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Jiang chuan mycin for preventing and controlling plant diseases

A technology of Jiangchuanmycin and plant diseases, applied in the field of pesticide technology and fermentation, can solve the problems of no one reporting the function of pesticides, unclear effects, loss of antibiotic activity, etc., to achieve convenient practical control operations, good resistance to rain erosion, and easy application good adaptability

Active Publication Date: 2013-04-17
德强生物股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, it is not clear whether this compound can play a role in the control of crop diseases and insect pests, and one is because its structure is involved in the loss of the antibiotic activity of blasticidin S Therefore, it is generally believed that it does not have antibiotic activity, so no one will spend energy on research; secondly, because of its low fermentation yield, it also limits people to study, so far no one has reported its pesticide function (see Canadian Journal of Chemistry (1994 ), 72(1):6-11; Phytochemistry (1993), 32(3):535-541; and, Journal of Antibiotics (1987), 40(12):1791-1793)

Method used

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  • Jiang chuan mycin for preventing and controlling plant diseases
  • Jiang chuan mycin for preventing and controlling plant diseases
  • Jiang chuan mycin for preventing and controlling plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Fermentation preparation and identification of Jiangchuanmycin

[0052] Inoculate the Streptomyces griseochromogenes strain CGMCC No. 6829, which the applicant deposited on November 15, 2012, at the General Microorganism Center (CGMCC) of the Chinese The weight percentage formula is: glucose 2%, starch 5%, peanut cake powder 2%, soybean cake powder 1%, peptone 0.1%, yeast extract 0.4%, sodium chloride 0.2%, magnesium sulfate 0.5%, ammonium nitrate 0.2% , disodium hydrogen phosphate 0.05%, pH 7.2, the balance is water), and cultured on a shaking table at 28° C. at 300 rpm for 64 hours to obtain a cultured seed solution.

[0053] The seed solution was inoculated into the fermentation medium in the fermenter with 10% inoculation amount (V / V) (the formula by weight percentage is: glucose 0.4%, starch 7%, peanut cake powder 1.5%, soybean cake powder 1.5% , 0.1% peptone, 0.4% yeast extract, 0.2% sodium chloride, 0.5% magnesium sulfate, 0.2% ammonium...

Embodiment 2

[0060] The mensuration of the antibacterial spectrum of embodiment 2 Jiangchuanmycin

[0061] Cultivate plant pathogenic microorganisms on medium mixed with different concentrations of Jiangchuanmycin (100, 50, 25mg / L), measure the colony growth diameter of each pathogenic bacteria, and control with each blank (the medium does not contain Jiangchuanmycin) Comparing to calculate inhibition rate. The measured pathogens except cucumber powdery mildew ( Sphaerotheca fuliginea , which will be studied in detail below), also includes: Xanthomonas oryzae (Pseudomonas oryzae), Erwinia carotovora (Cabbage Soft Rot), Bacillus subtilis (Bacillus subtilis), Eshchcrichia coli (Escherichia coli), Helmintosporium maydis (Maize subspecies fungus), Pyricuraria oryzae CaV (Ryza sativa), Colletotrichum lagenarium (Anthracnose of melons), Phoma asparagi saccardo (Asparagus Stem Blight), Helminthosporium oryzae (Rice flax fungus), Alternaria solani (Tomato blight fu...

Embodiment 3

[0066] Example 3 Control effect test of Jiangchuanmycin on melon anthracnose

[0067] Spray equal volumes of Jiangchuanmycin of different concentrations on a cucumber seedling at the true leaf stage in advance, and then artificially inoculate the anthracnose conidia suspension (spore concentration of 6.0×10 5 pcs / ml), and then put the cucumber seedlings in a constant temperature room (about 24°C) in a humidity chamber for shading and moisture induction. After 42 hours, take the cucumber seedlings out of the humidity chamber and place them under the light of a constant temperature room until the 7th day as a blank control ( (without pre-spraying Jiangchuanmycin) after the condition stabilized, count the number of anthracnose lesions on the leaves, compare with the blank control, calculate the control effect and further calculate the EC against melon anthracnose by linear regression method 50 value.

[0068] Table 2 Control effect of Jiangchuanmycin on melon anthracnose

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Abstract

The invention provides a Jiang chuan mycin for preventing and controlling plant diseases and a pesticide compound of the Jiang chuan mycin, which is used for disinfecting and / or resisting crop diseases. The pesticide compound has excellent effects for resisting plant pathogens such as phoma asparagi, pellicularia gramineum, vanturia pirina, saccharomycete, botrytis cinerea, helmintosporium maydis, pyricuraria oryzae, colletotrichum lagenarium and / or alternaria mali Roberts.

Description

technical field [0001] The invention belongs to the field of pesticide technology and fermentation technology, in particular, the invention relates to Jiangchuanmycin prepared by fermentation, which is used for preventing and treating plant diseases. Background technique [0002] Blasticidin S (Blasticidin S) was originally isolated from Streptomyces griseus ( Streptomyces griseochromogenes ) of agricultural antibiotics, mainly used to kill fungi. Many trace metabolic by-products are produced during the fermentation of blasticidin S, but they are rarely studied, especially for the compound whose structure is shown in the following formula (in this paper, referred to as "Jianchuanmycin") . [0003] . [0004] It is not clear whether this compound can play a role in the control of crop diseases and insect pests. In addition, because its structure is involved in the process of losing the antibiotic activity of blasticidin S, it is generally believed that it does not have a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N47/44A01P3/00A01P1/00C12P21/02C12R1/465
Inventor 孙新宇王敏刘伟杨鲁强田莉章丽
Owner 德强生物股份有限公司
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