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Double-layer electrospinning bionic periosteum and method for preparing same

A bionic bone and electrospinning technology, applied in textiles and papermaking, prosthesis, medical science, etc., to achieve the effect of enhancing the adhesion of MSCs

Inactive Publication Date: 2013-03-06
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the preparation of rFN / CDH and chitosan bilayer composite nanofibers by electrospinning

Method used

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  • Double-layer electrospinning bionic periosteum and method for preparing same
  • Double-layer electrospinning bionic periosteum and method for preparing same
  • Double-layer electrospinning bionic periosteum and method for preparing same

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Effect test

preparation example Construction

[0030] For the preparation of rFN / CDH fusion protein, please refer to the Chinese invention patent application number 200910103100.6, entitled "Fibronectin-cadherin-11 fusion protein, preparation method and application". The amino acid sequence of the FN / CDH fusion protein is shown in SEQ ID NO:1.

[0031] For electrospinning technology, please refer to Xu Songxiu's master's degree thesis "Research on Electrospinning Nanofibers"

Embodiment 1

[0033] Step 1: 0.8g of chitosan (molecular weight is 240000) is dissolved in 40ml concentration (by volume percentage) and is 25% glacial acetic acid aqueous solution, is made into the A chitosan solution that concentration is 2% (g / ml); The chitosan that 1.2g molecular weight is 88000 is dissolved in 40ml concentration (by volume percentage) is 50% glacial acetic acid aqueous solution, is made into the B chitosan solution that concentration is 3% (g / ml), microheating magnetic stirring 2 Obtained transparent light yellow chitosan glacial acetic acid solution in hours.

[0034] Step 2: Dissolve 10 mg of rFN / CDH fusion protein in 1 ml of deionized water, stir at room temperature until completely dissolved, and obtain a rFN / CDH protein solution with a concentration of 10 mg / ml.

[0035] Step 3: Prepare inner and outer membrane electrospinning liquid: mix B chitosan solution and rFN / CDH protein solution at a volume ratio of 5:1 to obtain a mixed solution of rFN / CDH protein and chi...

Embodiment 2

[0039] Step 1: 1.2g chitosan (molecular weight is 260000) is dissolved in 40ml concentration and is 25% glacial acetic acid aqueous solution, is made into the A chitosan solution that concentration is 2% (g / ml); 1.2g chitosan ( Molecular weight is 102000) be dissolved in 40ml concentration and be 50% glacial acetic acid aqueous solution, be made into the B chitosan solution that concentration is 3% (g / ml), microthermal magnetic stirring obtains transparent pale yellow chitosan glacial acetic acid solution for 2 hours.

[0040] Step 2: Dissolve 5 mg of rFN / CDH fusion protein in 1 ml of deionized water, stir at room temperature until completely dissolved, and obtain a rFN / CDH protein solution with a concentration of 5 mg / ml.

[0041] Step 3: Prepare inner and outer membrane electrospinning liquid: mix B chitosan solution and rFN / CDH protein solution at a volume ratio of 5:1 to obtain a mixed solution of rFN / CDH protein and chitosan, stir at room temperature to obtain inner membra...

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Abstract

The invention belongs to the field of tissue-engineered bones, and in particular relates to a method for preparing an electrospinning bionic periosteum. The method specifically comprises the steps that polysaccharide matrix solution and macromolecule protein are mixed and fully stirred, so that endosteum electrospinning liquid is obtained; the polysaccharide matrix solution is used as periosteum electrospinning liquid; an electrospinning device is applied to prepare periosteum electrospinning from the periosteum electrospinning liquid; and the bionic periosteum at least contains two layers, one layer is a bionic endosteum, and the other layer is a polysaccharide matrix periosteum which is prepared on the basis of electrospinning. According to the method, polysaccharide matrix is used as a carrier, can protect the endosteum and can prevent the interference factors of the peripheral environments, which are not beneficial to bone reconstruction; and according to the endosteum, recombinant fibronectin / cadherin chimera (rFN / CDH) fusion protein which can effectively enhance mesenchyal stem cells (MSCs) adhesion, multiplication efficiency and viability and is obtained after long-term work is buried in low molecular weight chitosan nanofibers through a blending mothod, and along with the degrading and absorbing of the chitosan fibers, the fusion protein rFN / CDH is exposed out of the surfaces of membrane materials.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular to the field of tissue engineering bone repair. Background technique [0002] "Electrospinning" is generally referred to as "electrospinning" or "electrospinning". In 1934, Formalas invented the experimental device for preparing polymer fibers by electrostatic force and applied for a patent. His patent disclosed how the polymer solution forms a jet between electrodes. This is the first patent describing in detail the use of high-voltage static electricity to prepare fiber devices. It is recognized as the beginning of the preparation of fibers by electrospinning technology. However, on a scientific basis, this invention can be considered a special case of electrostatic atomization, or electrospray, whose concept dates back to 1745. The biggest difference between electrostatic atomization and electrospinning is that they use different working media. Electrostatic atomization uses a low...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/22A61L27/20D04H1/4382D04H1/728
Inventor 李振声董世武杨晓占郭洪峰张瑗杨小超孙丽丽莫增
Owner ARMY MEDICAL UNIV
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