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Silk-fibroi and hyaluronic-acid (HA) composite gel for injection and preparation and application thereof

A composite gel and silk fibroin technology, applied in medical science, surgery, etc., can solve the problems of low hyaluronic acid content and poor water absorption of composite gel, achieve less inflammatory response, and improve in vitro anti-enzymatic hydrolysis ability. Effectiveness

Active Publication Date: 2012-12-26
ZHEJIANG XINGYUE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the composite gel prepared by the above method only relies on pure silk fibroin, or the physical interaction between silk fibroin and hyaluronic acid, the content of hyaluronic acid is less, and the water absorption of the composite gel is relatively poor.

Method used

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  • Silk-fibroi and hyaluronic-acid (HA) composite gel for injection and preparation and application thereof
  • Silk-fibroi and hyaluronic-acid (HA) composite gel for injection and preparation and application thereof
  • Silk-fibroi and hyaluronic-acid (HA) composite gel for injection and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: Silk fibroin hyaluronic acid composite gel

[0036] (1) Preparation of silk fibroin microspheres: Add 40g of silkworm silk to 2700ml of 0.2% sodium carbonate aqueous solution, bathe in water at 96-100°C for 60 minutes, filter, take the filter cake and repeat the water bath for 3 times, and take the last filtered After drying the filter cake at 60°C, dissolve it slowly with 200ml of 9mol / L lithium bromide aqueous solution, put it in a water bath at 60°C for 6h, then centrifuge at 8000rpm for 5min, take the supernatant, and put the centrifuged supernatant into a dialysis bag (MW8,000 ~14,000), with purified water as the dialysate, magnetic stirring and dialysis for three days, the retentate was taken, and a silk fibroin solution with a mass concentration of 5% was prepared with purified water.

[0037] At 25°C, let the above 5% silk fibroin solution stand still to form 5g of white gel material, homogenize the gel material with a homogenizer (T25, IKA) at 240...

Embodiment 2

[0040] Embodiment 2 silk fibroin hyaluronic acid composite gel

[0041] (1) According to the method described in step (1) of Example 1, 3.5 g of silk fibroin particles passed through a 200-mesh sieve were prepared, with a mass concentration of 3.23%.

[0042] (2) Dissolve 0.7g sodium hyaluronate in 7ml mass concentration of 1% sodium hydroxide solution to make 0.1g / ml sodium hyaluronate solution, and add the silk fibroin obtained in step (1) to the hyaluronic acid solution Protein particles 1.0836g (0.035 / 0.0323=1.0836g), mixed well, added 56μl BDDE, mixed evenly, placed at 40°C for 6 hours for cross-linking, forming silk fibroin hyaluronic acid composite gel material .

[0043] The above-mentioned silk fibroin hyaluronic acid composite gel material was subjected to dialysis treatment, and other operations were the same as in Example 1 to obtain 35 g of silk fibroin hyaluronic acid composite gel for injection, which can be used as a tissue filling injection product. Take the...

Embodiment 3

[0044] Embodiment 3: silk fibroin hyaluronic acid composite gel

[0045] (1) According to the method described in step (1) of Example 1, 3.5 g of silk fibroin particles passed through a 200-mesh sieve were prepared, with a mass concentration of 3.23%.

[0046] (2) Dissolve 0.7g sodium hyaluronate in 7ml mass concentration of 1% sodium hydroxide solution to make 0.1g / ml sodium hyaluronate solution, and add 2.167g (0.07 / 0.0323 =2.167g) of silk fibroin particles, mixed well, added 56 μl of BDDE, mixed evenly, and placed at 40°C for 5 hours for cross-linking to form a silk fibroin hyaluronic acid composite gel material.

[0047] The silk fibroin hyaluronic acid composite gel material was subjected to dialysis treatment, and other operations were the same as in Example 1 to obtain 35 g of silk fibroin hyaluronic acid composite gel for injection, which can be used as a subcutaneous filling injection product. Take the silk fibroin hyaluronic acid composite gel and dilute it with dis...

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Abstract

The invention discloses a silk-fibroi and hyaluronic-acid (HA) composite gel for injection and the preparation and application thereof. The composite gel is prepared from the following raw materials by weight: 1 part of silk fibroi microspheres, 1-50 parts of hyaluronate and 0.08-4 parts of crosslinking agent. Compared with the traditional hyaluronic-acid purely-crosslinked gel, due to the addition of the silk fibroi particles, the degradation rate of the composite gel is reduced effectively, the tissue repairing and filling time is prolonged and the effectiveness of the materials is improved, and due to the addition of the HA, the water absorption, the lubrication degree and the water storage function of the materials are improved. Compared with the silk fibroi particles and the HA particles mixed by adopting the pure physical method, because the silk fibroi particles are wrapped in the gel HA particles, the silk fibroi particles and the HA particles are mixed more uniformly, thus the prepared gel particles have low inflammatory response and good biocompatibility, and the safety of the materials is improved.

Description

(1) Technical field [0001] The invention relates to a medical cosmetic filling material, in particular to a silk fibroin hyaluronic acid composite gel for injection, its preparation method and its application in soft tissue filling material. (2) Background technology [0002] At present, in tissue repair and tissue enhancement, non-surgical treatment is growing rapidly, and the proportion has exceeded 80%. Since local injection is a minimally invasive treatment method, it is only necessary to inject certain drugs and chemical agents into the soft tissue with loose structure. Improve local laxity and local pressure tolerance, thereby restoring the tissue to its normal function or appearance. [0003] With the continuous development of science and technology, there are more and more tissue filling materials. According to their composition and biological characteristics, they can be divided into biomaterials and cell injection products. [0004] The use of cell injection is a ...

Claims

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Application Information

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IPC IPC(8): A61L31/04A61L31/14C08J3/075C08L89/00C08L5/08
Inventor 谢淑君陈隆坤赵洪石其他发明人请求不公开姓名
Owner ZHEJIANG XINGYUE BIOTECH
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