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Bionic specific immunity adsorbing material and preparation method as well as application thereof

An immunoadsorbent material and a specific technology, applied in the field of biomimetic specific immunoadsorbent material for blood purification and its preparation, can solve the problems of cumbersome activation steps, low reaction selectivity, high selectivity and high efficiency, etc. , to achieve mild reaction conditions, avoid side reactions, and improve the effect of coupling effect

Active Publication Date: 2015-06-03
杭州是派生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this preparation method, the activation steps are cumbersome, and after multi-step activation reactions, the total activation rate of the carrier is greatly reduced
Moreover, the reaction selectivity of the alkylamine bond to protein A is not high
Therefore, the reaction between the active carrier and the ligand is not highly selective and efficient, which greatly affects the adsorption performance of the immunoadsorbent material.

Method used

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  • Bionic specific immunity adsorbing material and preparation method as well as application thereof
  • Bionic specific immunity adsorbing material and preparation method as well as application thereof
  • Bionic specific immunity adsorbing material and preparation method as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Preparation of Azide Sepharose Gels

[0059] Add 4.0 g of drained agarose gel, 8 mL of epichlorohydrin and 4 mL of 3M sodium hydroxide solution into a 100 mL Erlenmeyer flask. After the mixture was stirred and reacted at room temperature for 3 h, it was washed with water and drained until the phenolphthalein did not turn red after adding 1.3M sodium thiosulfate to the washing liquid, and the epoxidized agarose was obtained. Dissolve 1.82g of sodium azide in 15mL of water, then add 4.0g of epoxidized agarose, and react at 20°C for 60h. After the reaction was completed, the gel was washed and dried to obtain an azide agarose gel. attached figure 1 The infrared spectrum shown is at 2100cm -1 The characteristic absorption peak of the azido group appeared at , which proved the existence of the azido group in the azide agarose.

[0060] Synthesis of Alkynoic Acids

[0061] 10.0 g of succinic anhydride, 1.01 g of triethylamine, 0.12 g of 4-dimethylaminopyridine and 50 mL ...

Embodiment 2

[0075] Preparation of Azide Sepharose Gels

[0076] Add 4.0 g of drained agarose gel, 8 mL of epichlorohydrin and 8 mL of 1.5 M sodium hydroxide solution into a 100 mL Erlenmeyer flask. After the mixture was stirred and reacted at room temperature for 4 h, it was washed with water and drained until the phenolphthalein did not turn red after adding 1.3M sodium thiosulfate to the washing liquid, thus obtaining epoxidized agarose. Dissolve 0.78g of sodium azide in 10mL of water, then add 4.0g of epoxidized agarose, and react at 40°C for 30h. After the reaction was completed, the gel was washed and dried to obtain an azide agarose gel.

[0077] Synthesis of Alkynoic Acids

[0078] 10.0 g of succinic anhydride, 10.1 g of triethylamine, 1.22 g of 4-dimethylaminopyridine and 250 mL of dichloromethane were placed in a 200 mL round bottom flask. At 10°C, 20 mL of a dichloromethane solution containing 16.8 g of propargyl alcohol was added dropwise. After the dropwise addition, the m...

Embodiment 3

[0092] Preparation of Azide Sepharose Gels

[0093] Add 4.0 g of drained agarose gel, 8 mL of epichlorohydrin and 6 mL of 2.5 M sodium hydroxide solution into a 100 mL Erlenmeyer flask. After the mixture was stirred and reacted at room temperature for 3.5 h, it was washed with water and drained until the phenolphthalein did not turn red after adding 1.3M sodium thiosulfate to the washing liquid, and the epoxidized agarose was obtained. Dissolve 3.9g of sodium azide in 25mL of water, then add 4.0g of epoxidized agarose, and react at 60°C for 6h. After the reaction was completed, the gel was washed and dried to obtain an azide agarose gel.

[0094] Synthesis of Alkynoic Acids

[0095] 10.0 g of succinic anhydride, 5.05 g of triethylamine, 0.61 g of 4-dimethylaminopyridine and 150 mL of dichloromethane were placed in a 200 mL round bottom flask. At 0°C, 15 mL of a dichloromethane solution containing 11.2 g of propargyl alcohol was added dropwise. After the dropwise addition, ...

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Abstract

The invention discloses a bionic specific immunity adsorbing material and a preparation method as well as application thereof. The preparation method comprises the following steps of: reacting epoxidation agarose with sodium azide to obtain agarose azide gel; dissolving succinic anhydride into methylene dichloride and propargyl alcohol and adding a catalyst to prepare acetylenic acid; dissolving the acetylenic acid and N-hydroxysuccinimide into 1,4-dioxane, and then adding a condensing agent to prepare active ester of the acetylenic acid; dissolving the active ester of the acetylenic acid into the 1,4-dioxane and then mixing with aqueous solution of aglycone to prepare ethynylation amino acid; dissolving solution of the ethynylation amino acid into agarose azide; and adding copper sulfate pentahydrate and sodium ascorbate as catalysts to prepare a product. According to the bionic specific immunity adsorbing material and the preparation method as well as application thereof, the agarose gel which is rich in hydroxyl groups and has good blood compatibility and mechanical property is used as a carrier, synthesis steps are simple, the preparation is safe, and a product has the characteristics of high specificity, high adsorption efficiency on IgG (Immunoglobulin G) and good regeneration performance and can be used for IgG separation and clinical immunoadsorption treatment.

Description

technical field [0001] The invention relates to a biomimetic specific immunoadsorption material, in particular to a biomimetic specific immunoadsorption material which can be used for blood purification and a preparation method thereof. The invention relates to the coupling of carrier and ligand by adopting click chemistry (click chemistry) method to prepare imitation biospecific immunoadsorption material. technical background [0002] Immunoadsorption therapy is a new technology based on the principle of affinity chromatography to achieve blood purification, which is used to treat some diseases that are difficult to be effective by traditional methods. It combines certain biologically active molecules with specific and reversible affinity as a ligand with a carrier to make an adsorption column, and uses its specific adsorption performance to specifically remove pathogenic factors in the patient's blood, thereby purifying the blood. , the purpose of preventing and treating ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/22B01J20/26B01J20/30
Inventor 李光吉胡小艳
Owner 杭州是派生物科技有限公司
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