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Method for preparing schaftoside from desmodium styracifolium

A technology of schaftoside and Desmodium sativa, applied in the field of natural medicinal chemistry, can solve the problems of poor reproducibility of silica gel column chromatography, unsuitability for the preparation of schiaftoside, complicated operation, etc., shorten the extraction cycle and increase the amount of preparation Great, easy-to-operate effects

Inactive Publication Date: 2012-08-08
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As mentioned above, the existing method is complicated to operate, especially the reproducibility of silica gel column chromatography is poor, the preparation amount is small, and the yield is low, so it is not suitable for the preparation of schaftosides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Dissolve 10g of cellulase in 5L of water, add 5kg of crushed Desmodium sativa, mix well, enzymatically hydrolyze at room temperature for 5 days, soak and extract the raw material for enzymolysis with warm water at 70°C for 2 times, add 40kg each time, filter the extract with molecular weight cut-off 2000 hollow cellulose ultrafiltration membrane for ultrafiltration, and the permeate was concentrated with a hollow cellulose nanofiltration membrane with a molecular weight cut off of 200. The obtained concentrated solution was extracted 3 times with an equal volume of ethyl acetate, and the extract was concentrated to obtain 20 g of crude extract. Mix ethyl acetate, n-butanol, and water in a ratio of 3:1:4. After fully layering, take the upper phase and fill the high-speed countercurrent chromatography column. At the same time, turn on the main engine at 900 rpm, pump the lower phase into the mobile phase, and wait for the system to balance Finally, the flow rate is adjuste...

Embodiment 2

[0020] Dissolve 10g of amylase 5 and pectinase in 5L of water, add 5kg of crushed Desmodium sativa, mix well, enzymatically hydrolyze at room temperature for 4 days, soak and extract the raw materials for enzymolysis with warm water at 60°C for 3 times, add 30kg each time, and extract After filtration, use a hollow cellulose ultrafiltration membrane with a molecular weight cut-off of 6000 for ultrafiltration, and then concentrate the permeate with a hollow cellulose nanofiltration membrane with a molecular weight cut-off of 300. The obtained concentrated solution is extracted 3 times with an equal volume of ethyl acetate, and the extract is concentrated to obtain Crude extract 12g. Mix ethyl acetate, n-butanol, and water in a ratio of 3:2:5. After fully layering, take the upper phase and fill the high-speed countercurrent chromatography column. At the same time, turn on the main engine at 800 rpm, pump the lower phase into the mobile phase, and wait for the system to balance F...

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Abstract

The invention discloses a method for preparing schaftoside from desmodium styracifolium. The method comprises the following steps of: i, crushing the desmodium styracifolium, adding biological enzyme and uniformly stirring, performing enzymolysis at normal temperature for 4-7 days, soaking enzymolysis raw material with warm water and extracting for two to three times, filtering extract and ultra-filtering with an ultrafiltration membrane, concentrating permeate with a nanofiltration membrane, extracting the concentrate by using acetic ether and concentrating the extract to obtain a rough extract; and ii, separating the rough extract by using high-speed counter-current chromatography, performing on-line monitoring through an ultraviolet detector, collecting a target component and performing reduced pressure drying to obtain the schaftoside. The method has the advantages of simple operation, high efficiency, large preparation capacity and high purity of an obtained product.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and in particular relates to a method for preparing schaftoside from Desmodium sativa. Background technique [0002] Desmodium sativa Desmodium styracifolium (Osb.) The above-ground part of Merr. is also called Desmodium, False Peanut, Mountain Bean. It has the effects of clearing heat and dehumidification, diuresis and treating stranguria. Traditional medicine is used to treat hot stranguria, sand stranguria, stone stranguria, painful urination, edema, oliguria, jaundice, red urine, and urinary tract stones. Modern studies have shown that Desmodium radix contains a large amount of flavonoids, which have significant anti-inflammatory, analgesic, and anti-platelet aggregation effects. Among them, schiaftoside is an index component in Desmodium sativa, and its content should not be less than 0.13% according to the Pharmacopoeia-2010 edition. [0003] Schaftoside is a flavonoid, CAS No....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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