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Method for producing lycopene by blocking metabolic pathway of Dunaliella bardawil

A technology of lycopene and pasteurian salina, applied in the field of food science, can solve the problems of difficult industrialized large-scale production, high growth environment requirements, cumbersome extraction process, etc., and achieves short extraction cycle, simple extraction method, and simple environmental requirements Effect

Active Publication Date: 2014-10-08
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, extraction from tomato epidermis has low extraction efficiency and cumbersome extraction process, which requires a large amount of tomatoes, which is very wasteful; B. trispora belongs to heterotrophic mold microorganisms, and the carbon source, nitrogen source and growth environment required for cultivation are high. , it is difficult to industrialize large-scale production, and the production cost is relatively high

Method used

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  • Method for producing lycopene by blocking metabolic pathway of Dunaliella bardawil
  • Method for producing lycopene by blocking metabolic pathway of Dunaliella bardawil
  • Method for producing lycopene by blocking metabolic pathway of Dunaliella bardawil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Preparation of Dunaliella culture solution

[0035] Preparation of salina culture medium: NaNO 3 0.420g / L, NaCl87.69g / L, NaH 2 PO 4 2H 2 O0.156g / L, NaHCO 3 0.840g / L, KCl0.074g / L, MgSO 4 ·7H 2 O1.230g / L, CaCl 2 2H 2 O0.044g / L, 0.1% EDTA iron 0.5ml / L. Add 1ml / L of A5 trace element solution to the culture medium to adjust the pH to 7.5. A5 trace element solution, its composition is as follows: H 3 BO 3 286,MnCl 2 4H 2 O181, ZnSO 4 ·7H 2 O22, CuSO 4 ·5H 2 O7.9, (NH 4 ) 6 Mo 7 o 24 4H 2 O3.9, the unit is mg / 100ml. Salina culture solution was divided into 500ml Erlenmeyer flasks by 100ml per bottle, sealed with four layers of gauze, and sterilized under high temperature at 100kPa for 20min. Use after cooling at room temperature.

[0036] (2) Inoculation culture

[0037] Centrifuge the algae solution in the algebraic growth phase at 5000r / min for 10min, discard the culture solution, add the algae cells into a Erlenmeyer flask filled with fresh cultur...

Embodiment 2

[0048] (1) Dunaliella culture fluid preparation (with embodiment 1)

[0049] (2) Inoculation culture

[0050] Same as Example 1, the difference is that the amount of blocking agent added is different, and the amount of triethylamine added in this embodiment is 200ppm.

[0051] (3) assay (with embodiment 1)

[0052] After adding triethylamine for 12 hours, there was no lycopene peak.

[0053] After adding triethylamine for 24 hours, the peak height of lycopene was 1.938mAU.

[0054] After adding triethylamine for 48 hours, the peak height of lycopene was 7.224mAU.

[0055] After adding triethylamine for 72 hours, the peak height of lycopene was 6.310mAU.

[0056] Among them, the peak height of lycopene reached a maximum value of 7.224mAU after adding the blocking agent for 48 hours.

Embodiment 3

[0058] (1) Dunaliella culture fluid preparation (with embodiment 1)

[0059] (2) Inoculation culture

[0060] Same as Example 1, the difference is that the amount of blocking agent added is different, and the amount of triethylamine added in this embodiment is 800ppm.

[0061] (3) assay (with embodiment 1)

[0062] After adding triethylamine for 12 hours, there was no lycopene peak.

[0063] After adding triethylamine for 24 hours, the peak height of lycopene was 1.598mAU.

[0064] After adding triethylamine for 48 hours, the peak height of lycopene was 4.357mAU.

[0065] Among them, the peak height of lycopene reaches the maximum value of 7.570mAU after adding the blocking agent for 72 hours.

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Abstract

The invention discloses a method for producing lycopene by bloking the metabolic pathway of Dunaliella bardawil. The method comprises the following steps: preparing a Dunaliella bardawil basic culturing solution, adding an A5 trace element solution to the culturing solution, adjusting the pH value, disinfecting, and cooling to room temperature; and 2, adding Dunaliella bardawil cells to the culturing solution, culturing in an illumination incubator, adding 200-800ppm of a blocker triethylamine when OD600 reaches 0.7, continuously culturing for 24-72h, and extracting a pigment to obtain lycopene. Compared with traditional methods for extracting lycopene from tomato skins, the extraction method disclosed in the invention has the advantages of simplicity, convenience, fastness and short extraction period. Compared with Blakeslea trispora and other autotrophic microorganisms, Dunaliella bardawil has the advantages of large-scale culture benefiting, simple requirements of environment required by culturing, and according with industrial large-scale production standards.

Description

technical field [0001] The invention belongs to the field of food science and technology. Specifically, it relates to a method for producing lycopene by blocking the metabolic pathway of Dunaliella salina with an blocking agent. Background technique [0002] Dunaliella salina (Dunaliella salina), a halophilic green microalgae, is a halophilic unicellular eukaryotic algae and is one of the most salt-tolerant eukaryotes discovered so far. It is a high-salt adversity organism with strong adaptability and fast reproduction. Its natural living environment is mostly salt ponds, salt lakes and other places. The special environment has created a unique growth mechanism. Dunaliella salina is a photoautotrophic organism rich in oil and carotene. Proteins, polysaccharides, etc., also contain high Ca, P, Zn and other minerals, and also contain 18 amino acids including human essential amino acids, and the accumulated glycerol is 40% to 50% of the dry weight. Under appropriate conditio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C12R1/89
Inventor 姜建国李一萌
Owner SOUTH CHINA UNIV OF TECH
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