Preparation method of nano-apatite/biopolymer composite stent with fluorescence labeling property

A biopolymer and fluorescent labeling technology, applied in the field of biomedical materials and optical functional materials, can solve problems such as difficult detection and diagnosis, and achieve the effects of wide range of uses, simple operation and luminescence performance.

Inactive Publication Date: 2012-07-18
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problems in the prior art that it is difficult to carry out effective detection and diagnosis of in vitro and in vivo tests, by developing a kind of rare earth ion doping with controllable morphology and crystallization degree, high dispersion and good biocompatibility The nano-apatite sol provides a preparation method for compounding the above-mentioned sol with collagen, chitosan and other biopolymer matrices to obtain a nano-apatite / biopolymer composite scaffold material with optical labeling properties, through the following technical scheme accomplish

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A. Add calcium hydroxide to the K15 type polyvinylpyrrolidone solution with a mass concentration of 0.01% to prepare a mixed solution I of calcium hydroxide and polyvinylpyrrolidone with a concentration of 0.01mol / L;

[0033]B. Add phosphoric acid to the K15 polyvinylpyrrolidone solution with a mass concentration of 0.01% to prepare a mixed solution II of phosphoric acid and polyvinylpyrrolidone with a concentration of 0.006mol / L;

[0034] C. The mixed solution I and mixed solution II obtained in steps A and B are mixed with ultrasonic treatment at a stirring speed of 100 rpm, and the pH value is controlled to 10 with ammonia water to form nano-hydroxyapatite sol III; at this time, nano-hydroxyphosphorus The molar ratio of calcium to phosphorus in limestone sol III reaches 1.6:1;

[0035] D. In the sol obtained in step C, adjust the pH value to 6 with hydrochloric acid with a concentration of 0.5 mol / L, and then press the molar ratio of europium ions: (europium ions...

Embodiment 2

[0043] A. Add calcium nitrate to the K30 type polyvinylpyrrolidone solution with a mass concentration of 0.5% to prepare a mixed solution I of calcium nitrate and polyvinylpyrrolidone with a concentration of 0.1mol / L;

[0044] B. Add disodium hydrogen phosphate to the K30 polyvinylpyrrolidone solution with a mass concentration of 0.5% to prepare a mixed solution II of disodium hydrogen phosphate and polyvinylpyrrolidone with a concentration of 0.06mol / L;

[0045] C. The mixed solution I and mixed solution II obtained in steps A and B are mixed with ultrasonic treatment at a stirring speed of 2000 rpm, and the pH value is controlled by sodium hydroxide to 11 to form nano-hydroxyapatite sol III; The molar ratio of calcium to phosphorus in hydroxyapatite sol III reaches 1.65:1;

[0046] D. In the sol obtained in step C, adjust the pH value to 6.2 with hydrochloric acid with a concentration of 0.8mol / L, and then press the molar ratio of terbium ions: (terbium ions+calcium ions...

Embodiment 3

[0054] A. Add calcium nitrate to the K60 type polyvinylpyrrolidone solution with a mass concentration of 2% to form a mixed solution I of calcium nitrate and polyvinylpyrrolidone with a concentration of 0.5mol / L;

[0055] B. Add dipotassium hydrogen phosphate to the K60 type polyvinylpyrrolidone solution with a mass concentration of 2% to prepare a mixed solution II of dipotassium hydrogen phosphate and polyvinylpyrrolidone with a concentration of 0.03mol / L;

[0056] C. The mixed solution I and mixed solution II obtained in steps A and B are mixed with ultrasonic treatment at a stirring speed of 5000 rpm, and the pH value is controlled to 11.5 with potassium hydroxide solution to form nano-hydroxyapatite sol III; at this time The molar ratio of calcium to phosphorus in nano-hydroxyapatite sol III reaches 1.67:1;

[0057] D. In the sol obtained in step C, adjust the pH value to 6.5 with hydrochloric acid with a concentration of 1 mol / L, and then add 0.05 mol / L of Thulium n...

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Abstract

The invention discloses a preparation method of a nano-apatite / biopolymer composite stent with fluorescence labeling property. The method comprises the following steps: preparing rare-earth doped nano-apatite sol, and performing post-treatment; and preparing a nano-apatite / biopolymer stent to obtain the nano-apatite / biopolymer composite stent with fluorescence labeling property. The stent has the advantages that the apatite particles have excellent dispersion in biopolymer matrix, and the stent has a light labeling property with optical stability and excellent luminescence performance, so that the problem that a conventional stent material difficultly performs effective detection, diagnosis and the like of in-vitro and in-vivo tests can be solved. The method has easiness in process and convenience for operation, and is easy for popularization and application.

Description

technical field [0001] The invention relates to the technical field of biomedical materials and photofunctional materials, in particular to a method for preparing a nano-apatite / biopolymer composite scaffold with photomarking properties. Background technique [0002] Bone tissue engineering scaffold material refers to a material that can be combined with osteoblasts and implanted into a living body. It is the most basic structure of tissue engineered bone and is considered to be the most promising one for bone repair and replacement therapy. biomaterials. At present, bone tissue engineering scaffold materials are mainly divided into three categories: biologically derived materials, ceramic materials and polymer materials, but there are some problems that make it difficult to apply, such as biologically derived materials often cause severe immune rejection, and hydroxyapatite is Ceramic materials such as ceramics have poor mechanical properties, and their degradation is diff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/50
Inventor 邱建备尹兆益宋志国杨正文周大成徐圆圆余雪
Owner KUNMING UNIV OF SCI & TECH
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