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Preparation method for sargassum fusiforme brown algae polyphenols

A technology of brown algae polyphenols and brown algae, which is applied in the field of functional health food or biomedicine, can solve the problems that it is not suitable for large-scale extraction, the relationship between the antioxidant activity and molecular weight of the extract is not clarified, and the extraction process is cumbersome.

Inactive Publication Date: 2012-07-18
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the alcohol extraction rate is high, the antibacterial activity of the product is good, and the pollution is small, but the amount of solvent is large; microwave extraction is considered to be the most efficient extraction method, which is energy-saving and environmentally friendly, but the investment is huge; enzymatic hydrolysis can better maintain the content of brown algae polyphenols. Active, but the extraction process is cumbersome and not suitable for a large amount of extraction
None of the extraction methods clarified the relationship between the antioxidant activity and molecular weight of the obtained extracts

Method used

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  • Preparation method for sargassum fusiforme brown algae polyphenols
  • Preparation method for sargassum fusiforme brown algae polyphenols
  • Preparation method for sargassum fusiforme brown algae polyphenols

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Embodiment 1

[0019] In the present embodiment, the hijiki collected in the sea area of ​​Naozhou Island, Zhanjiang City is a raw material, and the specific operation steps are as follows:

[0020] 1. Wash hijiki, dry at 40°C to constant weight, grind into powder and pass through a 20-mesh sieve for later use;

[0021] 2. Take 200 g of hijiki powder, add 2000 ml of 80% methanol aqueous solution, set it on a shaker at room temperature to avoid light for 3 times, each time for 24 hours, and concentrate under reduced pressure to remove methanol to obtain a methanol crude extract aqueous phase;

[0022] 3. The aqueous phase of the methanol crude extract was washed twice with an equal volume of n-hexane, and the aqueous phase was taken to obtain the aqueous phase of the crude extract, and then the aqueous phase of the crude extract was extracted twice with an equal volume of ethyl acetate to obtain an aqueous layer and acetic acid The ethyl ester layer extract was concentrated to a paste by rota...

Embodiment 2

[0025] In the present embodiment, the hijiki collected in the sea area of ​​Naozhou Island, Zhanjiang City is a raw material, and the specific operation steps are as follows:

[0026] 1. Wash hijiki, dry at 40°C to constant weight, grind into powder and pass through a 20-mesh sieve for later use;

[0027] 2. Take 500 g of hijiki powder, add 3000 ml of a 90% methanol aqueous solution, place it on a shaker at room temperature and protect from light for 3 times, 24 hours each time, and then concentrate under reduced pressure to remove methanol to obtain a methanol crude extract aqueous phase;

[0028] 3. The aqueous phase of the methanol crude extract was washed twice with an equal volume of n-hexane, the aqueous phase was taken to obtain the aqueous phase of the crude extract, and the aqueous phase of the crude extract was extracted twice with an equal volume of ethyl acetate to obtain an aqueous layer and ethyl acetate. Ester layer extract, concentrated to paste by rotary evapo...

Embodiment 3

[0031] In the present embodiment, the hijiki collected in the sea area of ​​Naozhou Island, Zhanjiang City is a raw material, and the specific operation steps are as follows:

[0032] 1. Wash hijiki, dry at 40°C to constant weight, grind into powder and pass through a 20-mesh sieve for later use;

[0033] 2. Take 1000 g of hijiki powder, add 8000 ml of a 70% methanol aqueous solution, set it on a shaker at room temperature to avoid light for 3 times, each time for 24 hours, and concentrate under reduced pressure to remove methanol to obtain a methanol crude extract aqueous phase;

[0034] 3. The aqueous phase of the methanol crude extract was washed twice with an equal volume of n-hexane, the aqueous phase was taken to obtain the aqueous phase of the crude extract, and the aqueous phase of the crude extract was extracted twice with an equal volume of ethyl acetate to obtain an aqueous layer and ethyl acetate. Ester layer extract, concentrated to paste by rotary evaporation;

...

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Abstract

The invention provides a preparation method of sargassum fusiforme brown algae polyphenols for a first time. Sargassum fusiforme brown algae polyphenols which belong to different molecular segments and have anti-oxidant activity are prepared by taking sargassum fusiforme as a raw material and adopting methods such as methanol extraction, extraction with different solvents, ultrafiltration interception molecular weight and the like. Obtained sargassum fusiforme brown algae polyphenol extracts include: water I (molecular weight is more than 30K), water II (molecular weight is more than 10K and less than 30K), water III (molecular weight is more than 5K and less than 10K), water IV (molecular weight is more than 5K), BI (molecular weight is more than 30K), BII (molecular weight is more than 10K and less than 30K), and BIII (molecular weight is more than 5K and less than 10K). Due to the adoption of these extracts obtained with the preparation method provided by the invention, the yield of the brown algae polyphenols is increased, the brown algae polyphenols have extremely high anti-oxidant activity, and a good basis is laid for the development and use of sargassum fusiforme.

Description

technical field [0001] The invention relates to the field of functional health food or biomedicine, in particular to a preparation method of hijiri fusiformis brown algae polyphenol. Background technique [0002] Sargassum fusiorme (Hary) Seichell belongs to the Phaeophyta, Sargassum family, and Sargassum. The algae are yellow-brown, thick and juicy, growing on rocks in the low tide zone, and are widely distributed from Liaodong Peninsula to Leizhou Peninsula. Sargassum has a long history of being used as medicine in my country, and the "Compendium of Materia Medica" records that it can "gall and condense qi, facilitate urination, treat beriberi, water and gas edema, and lack of food and drink". Modern medical research has shown that hijiki has a therapeutic effect on goiter and rheumatism, and can also lower blood lipids, resist thrombosis, eliminate brain fatigue, promote children's development, improve immunity and delay aging. It has therapeutic effects in medicine. A...

Claims

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Application Information

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IPC IPC(8): A61K36/03A61P39/06A23L1/29A23L33/00
Inventor 刘义卢虹玉杨小青
Owner GUANGDONG MEDICAL UNIV
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