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Preparation method and application of pilose antler stem cell exosome for resisting skin aging

An anti-aging, stem cell technology, applied in cell dissociation methods, preparations for skin care, epidermal cells/skin cells, etc., can solve problems such as stimulation of epithelial-mesenchymal cell transformation, immune rejection, and promotion of tumor cell metastasis

Pending Publication Date: 2020-10-27
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the direct use of stem cells has disadvantages in terms of storage and transportation inconvenience and immune rejection
Moreover, stem cells are easy to differentiate into other stromal cells, which may promote tumor cell metastasis and stimulate epithelial-mesenchymal cell transition, posing safety risks

Method used

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  • Preparation method and application of pilose antler stem cell exosome for resisting skin aging
  • Preparation method and application of pilose antler stem cell exosome for resisting skin aging

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Isolation and culture of velvet stem cells; velvet stem cells are derived from periosteum tissue of deer antler growth area, periosteum tissue of antler stalk or mesenchymal tissue of velvet antler.

[0032] (1) The specific operation process for collecting periosteum tissue from the antler area is as follows: anesthetize a 1-year-old male deer that has not yet begun to develop in the antler area. Next, incise the skin of the antler area, expose the periosteum of the antler area, clean up the loose connective tissue, and then use a clean scalpel to cut along the antler area (the lowest part of the extra ridge), and then use rat-tooth tweezers to cut the periosteum Tear it off from the bone, and quickly put it into the transport solution, which is DMEM medium containing double double antibody.

[0033] (2) The specific operation process for collecting the periosteum tissue of the peduncle is as follows: anesthetize a 2-year-old male deer, prepare the skin of the pedun...

Embodiment 2

[0046] (1) The activity of scavenging DPPH free radicals was detected on the exocrine of velvet antler stem cells obtained in Example 1.

[0047] Weigh 1 mg of DPPH and dissolve it in 20 ml of absolute ethanol, add 1 ml of DPPH solution and 0.01 ml of velvet antler stem cell exudate sample to each reaction system, mix well, and measure the absorbance value at 519 nm after standing for 30 min.

[0048] The blank medium was used as the blank control, and the sample control group was 1 mL of ethanol solution and 0.01 mL of velvet antler stem cell exudate samples. Calculate the DPPH free radical scavenging rate according to the following formula.

[0049] DPPH free radical scavenging rate=[1-(A sample group-A sample control group) / A blank control group] X100%

[0050] It is measured that the antler stem cell conditioned medium and its freeze-dried powder have obvious scavenging effect on DPPH free radicals, and the scavenging rate can reach APC-EXO: 77.4%, PPC-EXO: 75.3%, RMC-EXO...

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Abstract

The invention discloses a preparation method and application of a pilose antler stem cell exosome for resisting skin aging, and belongs to the technical field of tissue engineering. In order to solvethe problem of skin aging resistance, the invention provides an exosome which is the pilose antler stem cell exosome. The antler stem cell exosome contains a large amount of various components such ascytokines, growth factors, miRNA and mRNAs and is easily fused with cell membranes of adjacent cells, bioactive substances are selectively delivered to recipient cells, information transmission is performed among different cells, and signal transmission among the cells is regulated. Compared with human bone marrow mesenchymal stem cells, the antler stem cell exosome obtained by the method has theadvantages that pilose antler stem cells are easy to obtain, the proliferation speed is higher than that of the human bone marrow mesenchymal stem cells by more than ten times, DPPH free radicals canbe removed, and the antler stem cell exosome has the anti-oxidation function and has the obvious promotion effect on the proliferation of Hacat cells and NHDF cells.

Description

technical field [0001] The invention relates to a preparation method and application of antler stem cell exosomes for anti-aging skin, and belongs to the technical field of tissue engineering. Background technique [0002] Antler is the only known mammalian organ that can fully regenerate, and its growth rate can reach 2cm / d. The complete regeneration and rapid growth ability of deer antler is mainly due to the presence of deer antler stem cells. It has been identified that antler stem cells are a special stem cell type between embryonic stem cells and mesenchymal stem cells. Compared with bone marrow mesenchymal stem cells, umbilical cord mesenchymal stem cells and other stem cells commonly used at present, antler stem cells are easier to obtain, proliferate faster, and secrete a large amount of cytokines and biologically active factors. [0003] In recent years, the use of stem cell transplantation to supplement human stem cells for anti-aging is considered to be a new a...

Claims

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Application Information

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IPC IPC(8): C12N5/071A61K8/98A61Q19/08
CPCC12N5/063A61K8/985A61Q19/08C12N2509/00C12N2509/10A61K2800/805
Inventor 孙红梅赵海平王大涛刘振郭倩倩
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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