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Ion chromatography-ultraviolet detection method for determining benazepril content in drug

A technology of ion chromatography and external detection, which is used in measurement devices, material separation, instruments, etc., to achieve good reproducibility, short analysis time, and good peak shape.

Inactive Publication Date: 2012-07-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no report on the determination of benazepril drug content by ion chromatography-ultraviolet detection

Method used

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  • Ion chromatography-ultraviolet detection method for determining benazepril content in drug
  • Ion chromatography-ultraviolet detection method for determining benazepril content in drug
  • Ion chromatography-ultraviolet detection method for determining benazepril content in drug

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Embodiment Construction

[0017] The technical scheme of the present invention is further specifically described below by specific examples:

[0018] Determination of Benazepril in Benazepril Hydrochloride Tablets by Ion Chromatography-Ultraviolet Detection

[0019] 1. Preparation of standard solution: Accurately weigh 10 mg of benazepril standard sample, add 50% acetonitrile into a 10 ml volumetric flask, shake to dissolve, and set the volume to the mark;

[0020] 2. Preparation of standard curve: prepare standard solutions with different concentrations by step-by-step dilution;

[0021] 3. Sample pretreatment: Remove the tablet coating, grind into powder, add 50% acetonitrile after accurate weighing to dissolve, sonicate for 15 minutes, wait for cooling to room temperature, quantify to the scale line, dilute to a suitable concentration, and filter through a 0.45 μm filter membrane ;

[0022] 4. Selection of eluent: test different ratios of eluent, and finally determine that: 35% acetonitrile + 12% ...

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Abstract

The present invention provides a new detection method for benazepril. According to the method, the combination of an ion chromatography cation exchange system and an ultraviolet detector is adopted, suitable separation conditions are selected according to the difference of retention behaviors of the materials on the chromatographic column, the optimal ultraviolet detection wavelength is selected, and the benazepril can be separated and detected at a high sensitivity. The method of the present invention can be used for detection of the benazepril content in the benazepril hydrochloride drug, human body fluid pharmacokinetic research, and the like.

Description

technical field [0001] The invention relates to an application system for chemical instrument analysis, in particular to an ion chromatography-ultraviolet detection system for detecting benazepril. Background technique [0002] Benazepril belongs to angiotensin-converting enzyme inhibitors (ACEI) and is a kind of antihypertensive drugs. Non-specific side effects include gastrointestinal reactions such as nausea and vomiting, and nervous system side effects such as headache and dizziness. Common analysis methods mainly use HPLC UV detection and HPLC-MS detection. When separating by HPLC, it is necessary to adjust the pH value of the mobile phase to about 3, while the silica gel column cannot withstand strong acid and strong alkali, and the structure of benazepril contains amino groups, which exist in the form of organic cations in an acidic environment, and are more suitable for separation by ion chromatography. So far, there is no report on the determination of benazepril ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 周丹刘玉秀朱岩
Owner ZHEJIANG UNIV
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