Preparation of water soluble saccharide fluorescent molecular probe and rapid detection for iron ions in water and biological tissue

A fluorescent molecular probe, biological tissue technology, applied in material separation, luminescent materials, measurement devices, etc., can solve the problems of expensive detection, poor water solubility, and insufficient detection speed, and achieves easy synthesis, reduced pollution, and simple synthesis. Effect

Inactive Publication Date: 2012-07-04
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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Problems solved by technology

It has been reported in the literature that the synthesis method of fluorescent molecular probes for iron ions is complex and costly. The detection of iron ions by the synthesized products is outside the visible spectrum range, and the color change cannot be observed by the naked eye, and expensive detection instruments must be used.
In addition, there are still disadvantages such as sample pretreatment, insufficient detection speed, poor water solubility, and expensive detection.

Method used

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  • Preparation of water soluble saccharide fluorescent molecular probe and rapid detection for iron ions in water and biological tissue
  • Preparation of water soluble saccharide fluorescent molecular probe and rapid detection for iron ions in water and biological tissue
  • Preparation of water soluble saccharide fluorescent molecular probe and rapid detection for iron ions in water and biological tissue

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Experimental program
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Effect test

Embodiment 1

[0010]

[0011] Compound 1 (1.5 g) was dissolved in trifluoroacetic acid, and hexamethylenetetramine (1.9 g) was added at room temperature to react for about 10 hours. The progress of the reaction was monitored by thin layer chromatography. After the reaction was complete, it was concentrated and purified on a silica gel column. Ethyl acetate and petroleum ether were used as eluents to obtain compound 2 (800 mg, 57%).

Embodiment 2

[0013]

[0014] Dissolve compound 2 (350 mg) in water, add D-glucosamine hydrochloride (796 mg) and sodium hydroxide (67 mg) at room temperature, and react at a certain temperature for about 5 hours. Then, it was filtered, washed, and the solid was collected to obtain compound 3 (592 mg, 97%), which is a fluorescent molecular probe for carbohydrates.

Embodiment 3

[0016] Equimolar amounts of carbohydrate fluorescent molecular probes and various metal ions (Na + 、Ag + 、Cat 2+ , Hg 2+ , Mg 2+ , Mn 2+ 、Cu 2+ , Pb 2+ 、Cd 2+ 、Cr 2+ , Fe 2+ , Fe 3+ , Zn 2+ 、Ni 2+ ) respectively dubbed into 10 -4 The solution of M is then mixed, and the color change of the solution is observed. The color of the fluorescent molecular probe solution is yellow. After the fluorescent molecular probes were added to various ionic solutions, the color of the iron ion solution changed from yellow to reddish-brown quickly, and the colors of other solutions hardly changed or did not change significantly. Through this experiment, it can be seen that the synthesized sugar fluorescent molecular probe has good selectivity to iron ions, and other metal ions will not cause interference.

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Abstract

The invention provides a preparation of a water soluble saccharide fluorescent molecular probe as well as a direct and rapid detection for iron ions in water and a biological tissue. The fluorescent molecular probe obtains very high selectivity and sensitivity for iron ions in water and serum; after the fluorescent molecular probe is selectively combined with the iron ions, the color change of the solution from yellow to red brown can be observed by naked eyes; and the lower detection limit can reach 10-6 M. Without need of instruments, the field real-time direct and rapid detection can be carried out. The fluorescent molecular probe can be prepared to obtain an iron ion detecting test paper and a detecting kit and can be also coated with films to obtain a portable solid detector by using viscosity of saccharide compounds. The specific structure of the saccharide fluorescent molecular probe (taking a glucosamine ligand for example): except for glucosamine, the saccharide ligand can be also other saccharide compounds and derivatives thereof.

Description

technical field [0001] The invention relates to the preparation of a class of water-soluble carbohydrate fluorescent molecular probes and the rapid detection of iron ions in water and biological tissues. Background technique [0002] Iron ion is one of the ions necessary for living organisms. It is a necessary substance for the manufacture of heme and myoglobin and the promotion of vitamin B metabolism. Many biological and chemical processes at the cellular level require the participation of iron ions, so the research can be used for iron ions The detected fluorescent molecular probes are of great significance to the environment and life sciences. It is reported in the literature that the synthesis method of fluorescent molecular probes for iron ions is complex and costly. The detection of iron ions by the synthesized products is outside the visible spectrum range, and the color change cannot be observed with the naked eye, and expensive detection instruments must be used. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N21/78G01N30/02G01N30/90
Inventor 魏国华孙艳灵杜宇国
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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