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Method for using ethanol two-aqueous-phase method for extracting seaweed phycobiliprotein

A phycobiliprotein and aqueous two-phase technology is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., and can solve the problems of unsuitability for large-scale production, high residual concentration of ammonium sulfate, and long dialysis treatment time. Achieve the effect of being beneficial to industrial scale production, improving cell fragmentation rate and retaining biological activity

Inactive Publication Date: 2012-07-04
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The saturated ammonium sulfate gradient precipitation method requires a large amount of ammonium sulfate, and the residual concentration of ammonium sulfate in the finished product is high. The subsequent treatment method for removing ammonium sulfate adopts ultrafiltration or dialysis. Ultrafiltration requires high equipment requirements and is not suitable for large-scale production; Dialysis takes a long time to process, consumes a lot of water, and is not environmentally friendly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, a kind of method utilizing ethanol two-phase method to extract laver phycobiliprotein, carries out following steps successively:

[0030] (1), dried laver crushed and soaked:

[0031] Get 50 grams of dried laver (water content 9.4%, protein content 30.35%), use pulverizer to grind dry laver into powder less than 20 meshes, add distilled water according to solid-liquid ratio 1g / 15ml and soak for 30min, obtain pulpy laver suspension;

[0032] (2), mechanical crushing, extraction and separation of wet laver:

[0033] Adjust the gap between the colloid mill discs to 5 μm and then process the pulpy laver suspension for 1 min, and centrifuge the obtained juice at 4000 rpm for 12 min to obtain supernatant I and precipitation respectively;

[0034] (3), adding water to the precipitation, and extracting and separating by ultrasonic waves:

[0035] Add 25ml of distilled water to the precipitate obtained in step (2), stir evenly, treat with 500W ultrasonic wave for...

Embodiment 2

[0047] Embodiment 2, a kind of method utilizing ethanol two-phase method to extract laver phycobiliprotein, carries out following steps successively:

[0048] (1), dried laver crushed and soaked:

[0049] Take 50 grams of dried laver (water content 9.4%, protein content 30.35%), use a pulverizer to crush the dried laver into a powder smaller than 20 meshes, add distilled water to soak for 30 minutes according to the solid-to-liquid ratio of 1g / 17ml, and obtain a pulpy laver suspension;

[0050] (2), mechanical crushing, extraction and separation of wet laver:

[0051] After adjusting the gap between the colloidal grinding discs to 5 μm, the pulpy laver suspension was processed for 2 minutes, and the obtained juice was centrifuged at 4000 rpm for 12 minutes to obtain supernatant I and precipitation respectively;

[0052] (3), adding water to the precipitation, and extracting and separating by ultrasonic waves:

[0053] Add 50ml of distilled water to the precipitate obtained i...

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Abstract

The invention discloses a method for using an ethanol two-aqueous-phase method for extracting seaweed phycobiliprotein, which sequentially comprises the following steps of (1) smashing dry seaweed and then adding distilled water for soaking; (2) using a colloid mill for processing pulpiness seaweed suspension liquid, separating, and respectively obtaining liquid supernatant I and sediment; (3) conducing extraction separation on obtained sediment by adding water to obtain liquid supernatant II; (4) combining the liquid supernatant I and the liquid supernatant II, adding ammonium sulfate, and centrifuging after stewing; and (5) adding ammonium sulfate and ethanol in obtained liquid supernatant III, stewing, obtaining the intermediate phase, enabling the seaweed phycobiliprotein obtained in the step (5) to be subsided, washing, drying, smashing, and obtaining the seaweed phycobiliprotein. The method is used for extracting the seaweed phycobiliprotein, and has the advantages of being concise in process, high in obtaining rate and the like.

Description

technical field [0001] The invention relates to the technical field of food preparation, in particular to a method for extracting laver phycobiliproteins by an ethanol two-phase method. Background technique [0002] Laver (Porphyra) has always been paid attention to because of its high nutritional value and important health functions. The protein content of Porphyra is 30% of the dry weight, most of which are phycobiliproteins. [0003] Phycobiliproteins consist of phycoerythrin, phycocyanin, and allophycocyanin. The application of phycobiliprotein in medicine and food industry has attracted widespread attention, and high-purity phycobiliprotein has been applied in the pharmaceutical industry. At present, the extraction and development of phycobiliproteins are mainly extracted from algae such as spirulina. The price of raw materials is relatively expensive, the extraction cost is high, the subsequent purification process is complicated, and industrial production is difficul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/30C07K1/14
Inventor 陈新峰周利根张治国王君虹
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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