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Gingkgo active ingredient extraction and separation method and use of extracted active matters in medicines for treating cardiovascular and cerebrovascular diseases

A technology of active ingredients and separation method, applied in the field of traditional Chinese medicine technical preparation, can solve the problem that the treatment mechanism is not very clear and the like

Active Publication Date: 2012-04-18
XUZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ginkgo biloba extract has the effect of expanding coronary arteries and cerebrovascular, can improve microcirculation, and promote the metabolism of heart and brain tissue. Ginkgo biloba preparations have been used clinically to treat cardiovascular diseases for many years. For example, it can treat cardiovascular diseases such as hypertension. The treatment mechanism is not very clear

Method used

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  • Gingkgo active ingredient extraction and separation method and use of extracted active matters in medicines for treating cardiovascular and cerebrovascular diseases
  • Gingkgo active ingredient extraction and separation method and use of extracted active matters in medicines for treating cardiovascular and cerebrovascular diseases
  • Gingkgo active ingredient extraction and separation method and use of extracted active matters in medicines for treating cardiovascular and cerebrovascular diseases

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Experimental program
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Effect test

Embodiment 1

[0036] 1) Ginkgo dry leaves are crushed, 100 g of ginkgo leaf powder (particle size Φ0.8-2.0 mm) is taken, and 70% ethanol is extracted twice with 7 times of micro-boiling, each extraction is 2 hours, and the quality of the extract is 40.1 g. The extraction rates of flavonoids and lactones are 80.8% and 77.2% respectively

[0037] 2) Get about 1000ml of ginkgo biloba ethanol extraction filtrate, reclaim ethanol by distillation under reduced pressure, sink and filter with water, put a mass of 500g macroporous resin D101 adsorption column at a flow rate of 1BV / h, wash the column with 1000ml water, and then use 70% mass fraction of Gradient elution with ethanol aqueous solution, collecting the eluent, recovering ethanol under reduced pressure, and evaporating to dryness, the total flavonoid purity of the obtained crude extract reaches 25.6%, and the total lactone purity reaches 6.3%.

[0038] 3) Extraction method to remove acid, take 200ml of the macroporous resin eluate obtained...

Embodiment 2

[0044] Embodiment 2 (application test):

[0045] (1) Study on the general level of adult rats

[0046] Adult SD rats (provided by the Experimental Animal Center of Xuzhou Medical College), weighing 200-250 g, male or female, aged 4-6 months, were randomly divided into 4 groups (7-11 rats in each group). Normal saline group; DMSO control group; bilobalide group; propranolol group. Rats were anesthetized by intraperitoneal injection of 10% chloral hydrate 4ml / kg. A cannula was inserted through the common carotid artery, connected to a pressure transducer, and connected to the MedLab-U / 4C501 biological signal acquisition and processing system. Bilobalide (purity up to 98.5%) is configured with DMSO solution, and the amount of adding DMSO is no more than 1‰ each time, and is given by femoral vein injection with the concentration of 2 mg / kg bilobalide 10-2mol / L; propranolol (purchased (from Sigma Company) was prepared with normal saline, and injected with 0.3 mg / kg propranolol a...

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Abstract

The invention discloses a gingkgo active ingredient extraction and separation method and use of extracted active matters in medicines for treating cardiovascular and cerebrovascular diseases. The method comprises the following steps: 1) grinding gingkgo leaves, and extracting gingkgo leaf active ingredients, namely flavone and terpene lactone compounds; 2) removing ginkgolic acids; separating gingkgo flavones from terpene lactones; and 4) purifying the terpene lactones by high performance liquid chromatography. The tests of effects of high-purity gingkgo terpene lactone medicines on the heart function of adult and old rats at organ, body and cell levels prove gingkgo terpene lactones have protective effects on the heart of Sprague Dawley (SD) adult and old rats. The method has the advantage that four terpene lactones with high purity of over 98 percent are prepared by high performance liquid chromatography. The ginkgoic acid content in the gingkgo leaf extract is controlled to be within 1ppm, the separation of flavones from terpene lactones is realized, the content of gingkgo flavones after separation reaches over 55 percent, and the gingkgo terpene lactone content reaches over 25 percent. All of the three indexes reach national standards.

Description

technical field [0001] The invention relates to a method for extracting active ingredients from plants, in particular to a method for extracting effective active ingredients flavonoids and terpene lactones from ginkgo leaves, and using the effective extract ginkgolide to study its effect on body blood pressure, heart blood flow, etc. The kinetics and the influence of the contraction force of a single cardiomyocyte belong to the technical preparation field of traditional Chinese medicine. Background technique [0002] Cardiovascular and cerebrovascular diseases are the main diseases that endanger human health, and their morbidity and mortality are increasing year by year. Whether in my country or Western developed countries, they have surpassed tumors and become the first cause of human death. [0003] The active ingredients of Ginkgo biloba preparations are mainly flavonoids and terpene lactones, referred to as Ginkgo biloba extract (GbE). Terpene lactone compounds can be fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16A61P9/00A61K127/00
Inventor 孙红李保民刘勇林布会敏
Owner XUZHOU MEDICAL COLLEGE
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