Wikstroemia indica (L.) C.A.Mey extract as well as preparation method and application thereof
A technology of extract and Gewang, which is applied in the field of medicine, can solve the problems of failing to give full play to Gewang, not clarifying the active ingredients of Gewang extract, and not being clear about the material basis of medicinal effects
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Embodiment 1
[0038] Embodiment 1 The preparation of Gewang extract
[0039] Gewang extract was prepared as follows:
[0040] (1) After taking 1000 g of the aerial part of Gewang and pulverizing it (about 1 cm in length), adding 8 L of ethanol with a concentration of 95% by volume and extracting at room temperature for 2 hours, concentrating the extract under reduced pressure at 40°C and drying it to obtain 80 g of the extract;
[0041] (2) Extract 80 g of the extract three times with 150 mL of petroleum ether, 150 mL of chloroform, 150 mL of ethyl acetate, and 150 mL of n-butanol, regardless of the order of extraction, or use one or two or more extraction solvents to extract separately;
[0042] (3) Get ethyl acetate extract or n-butanol extract and carry out silica gel column chromatography, through chloroform: methanol (100: 1-1: 1, volume ratio) gradient elution; Collect chloroform: methanol: water volume ratio is 50:1 to 1:1 eluted components, to obtain the effective part; the effecti...
Embodiment 2
[0056] Embodiment 2 The preparation of Gewang extract
[0057] Gewang extract was prepared as follows:
[0058] (1) After taking 1000 g of the aerial part of Gewang and pulverizing it (about 1 cm in length), adding 8 L of ethanol with a concentration of 95% by volume and extracting at room temperature for 2 hours, the extract was concentrated at 40° C. under reduced pressure and dried to obtain 85 g of extract;
[0059] (2) Extract 80g of the extract with 300mL of petroleum ether, 300mL of chloroform, 300mL of ethyl acetate, and 300mL of n-butanol, respectively, for 4 times. extraction;
[0060] (3) Take ethyl acetate extract or n-butanol extract and carry out silica gel column chromatography, through chloroform: methanol: water (100: 1: 0-1: 1: 1, volume ratio) gradient elution; Collect chloroform: Methanol: water volume ratio is 100: 1 to 50: 1 elution component, obtains effective part; Effective part continues through chloroform: methanol (100: 1-1: 1, volume ratio) gradi...
Embodiment 3
[0064] Embodiment 3 The preparation of Gewang extract
[0065] Gewang extract was prepared as follows:
[0066] (1) After taking 1000 g of the aerial part of Gewang and pulverizing it (about 1 cm in length), adding 8 L of ethanol with a concentration of 95% by volume and extracting at room temperature for 2 hours, concentrating the extract under reduced pressure at 40°C and drying it to obtain 80 g of the extract;
[0067] (2) Extract 80g of the extract with 500mL of petroleum ether, 500mL of chloroform, 500mL of ethyl acetate, and 500mL of n-butanol, respectively, for 4 times. extraction;
[0068] (3) Take ethyl acetate extract or n-butanol extract and carry out silica gel column chromatography, through chloroform: methanol: water (100: 1: 0-1: 1: 1, volume ratio) gradient elution; Collect chloroform: Methanol: the eluting fraction of water volume ratio is 50:1 to 1:1, obtains effective fraction; Effective fraction continues to pass through chloroform:methanol (30:1-1:1, vo...
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