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Preparation method of molecularly imprinted polymer for detecting lung cancer tumor markers

A technology of tumor markers and molecular imprinting, which is applied in the fields of material chemistry and electrochemistry, can solve the problems of not reaching the protein level and relatively little research on protein molecular imprinting, and achieve convenient, rapid, effective, high specificity, and simple operation Effect

Inactive Publication Date: 2011-12-07
中南大学肝胆肠外科研究中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using "artificial antibodies" such as MIPs to replace natural antibodies to achieve accurate detection has been successful in the detection of many small molecules (vitamins, amino acids, aldehydes, coenzymes, sterols, drug morphine, etc.), but for proteins and other large The molecular imprinting of molecules has been less studied. So far, the substrates used by MIPs studied by scientists can only reach the peptide level (below 3kD), and have not reached the protein level (above 20kD).

Method used

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  • Preparation method of molecularly imprinted polymer for detecting lung cancer tumor markers
  • Preparation method of molecularly imprinted polymer for detecting lung cancer tumor markers
  • Preparation method of molecularly imprinted polymer for detecting lung cancer tumor markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Molecularly imprinted polymers for detection of CEA

[0037] (1) Electrochemical modification of CEA

[0038] Take 4mg of ferrocene (FER) formate, dissolve in 800μL Na-HEPES buffer (0.15M, pH7.3), filter the solution through a 0.22μm microporous membrane, add 10mg (1-ethyl-3-[3- Dimethylaminopropylamine] carbodiimide-hydrochloric acid) (EDC) and 90 μL of 500 μ g / mL template molecule CEA and incubated at room temperature for 4 hours, ultrafiltration to remove unbound ferrocene formate until the filtrate no longer contains ferrocene formate. The prepared template complexes were stored at 4°C.

[0039] (2) Synthesis of molecularly imprinted polymers

[0040] The molecularly imprinted polymer is prepared by free radical polymerization induced by ultraviolet light. The template molecule is the template molecule obtained in (1), the functional monomer is ethyl dimethylol acrylate (EGDMA), the crosslinking agent is diacryloylpiperazine, the porogen is methanol, ...

Embodiment 2

[0043] Example 2 Molecularly imprinted polymer for detecting NSE

[0044] (1) Electrochemical modification of NSE

[0045] Take 4mg of ferrocene (FER) formate, dissolve in 800μL Na-HEPES buffer (0.15M, pH7.3), filter the solution through a 0.22μm microporous membrane, add 10mg (1-ethyl-3-[3- Dimethylaminopropylamine] carbodiimide-hydrochloric acid) (EDC) and 90 μL of 500 μ g / mL template molecule NSE and incubate at room temperature for 4 hours, and remove unbound ferrocene formate by ultrafiltration until the filtrate no longer contains ferrocene formate. The prepared template complexes were stored at 4°C.

[0046] (2) Synthesis of molecularly imprinted polymers

[0047] The molecularly imprinted polymer is prepared by free radical polymerization induced by ultraviolet light. The template molecule is the template molecule obtained in (1), the functional monomer is ethyl dimethylol acrylate (EGDMA), the crosslinking agent is diacryloylpiperazine, the porogen is methanol, a...

Embodiment 3

[0050] Example 3 Molecularly imprinted polymers for detection of CYFRA21-1

[0051] (1) Electrochemical modification of CYFRA21-1

[0052] Take 4mg of ferrocene (FER) formate, dissolve in 800μL Na-HEPES buffer (0.15M, pH7.3), filter the solution through a 0.22μm microporous membrane, add 10mg (1-ethyl-3-[3- Dimethylaminopropylamine] carbodiimide-hydrochloric acid) (EDC) and 90 μL of 500 μ g / mL template molecule CYFRA21-1 and incubated at room temperature for 4 hours, ultrafiltration to remove unbound ferrocene formate until the filtrate no longer contains ferrocene formate. The prepared template complexes were stored at 4°C.

[0053] (2) Synthesis of molecularly imprinted polymers

[0054] The molecularly imprinted polymer is prepared by free radical polymerization induced by ultraviolet light. The template molecule is the template molecule obtained in (1), the functional monomer is ethyl dimethylol acrylate (EGDMA), the crosslinking agent is diacryloylpiperazine, the por...

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Abstract

The invention discloses a preparation method of a molecular imprinted polymer for detecting lung cancer tumor markers. The method comprises the following basic process of: dissolving template molecules (ferrocenecarboxylic acid-tumor marker), a functional monomer and a cross-linking agent in an organic solvent (pore-foaming agent); then transferring the solution into water, stirring and emulsifying; adding an initiator for cross-linking, performing ultraviolet polymerization to obtain spherical molecular imprinted substances with relatively uniform particle sizes; and washing by ethanol, and centrifuging to remove the template molecules to obtain the molecular imprinted polymer with specificity to the lung cancer tumor markers. The molecular imprinted polymer is also used as an identifying element of a biosensor. The invention provides a new tumor diagnosis method.

Description

technical field [0001] The invention relates to the fields of material chemistry and electrochemistry, in particular to a method for preparing a molecularly imprinted polymer for detecting lung cancer tumor markers. Background technique [0002] The occurrence and proliferation of tumors is a gradual evolution process. During this gradual change process, the abnormal secretion or shedding of malignant tumor cells, or the body's tumor response can enter the blood or body fluids, which is closely related to tumor occurrence and proliferation. Substances are called tumor markers (Tumor markers, TM), which are roughly divided into six categories: carcinoembryonic antigens, enzymes, hormones, glycoproteins, oncogenes, and cell surface tumor antigens. The dynamic monitoring and determination of such substances can provide reliable basis for auxiliary diagnosis, differential diagnosis, curative effect observation, condition monitoring and prognosis evaluation of tumors. At present...

Claims

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Application Information

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IPC IPC(8): C08F220/28C08F220/14C08F212/14C08F2/44C08J9/26G01N33/574G01N27/26
Inventor 李异凡张阳德李辉莹陈福韬刘殿奎
Owner 中南大学肝胆肠外科研究中心
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