Preparation method of magnetic immobilized enzyme for producing plant sterol ester

Abstract

The invention relates to a preparation method of a magnetic immobilized enzyme for producing a plant sterol ester, belonging to the field of catalytic materials and chemical synthesis. The preparation method of the magnetic immobilized enzyme for producing the plant sterol ester is characterized by comprising the following steps: 1) carrying out chemical modification on a magnetic substrate with vinylphosphonic acid to introduce a carbon-carbon double bond with reactivity onto the surface of the magnetic substrate; 2) wrapping the surfaces of the modified magnetic particles with polymer layers containing epoxy or sulfonate radical groups to obtain magnetic polymer microspheres; and 3) in a buffer solution, immobilizing an enzyme on the surfaces of the magnetic polymer microspheres by an epoxy ring-opening or electrostatic adsorption method to obtain the magnetic immobilized enzyme for producing the plant sterol ester. The obtained magnetic immobilized enzyme has the characteristics ofhigh catalytic activity, good reuse performance and is simple in separation and recovery.

Description

technical field [0001] The invention relates to a method for preparing magnetically immobilized enzymes for producing phytosterol esters, belonging to the field of catalytic materials and chemical synthesis. Background technique [0002] Phytosterols have the effect of lowering serum cholesterol levels and preventing cardiovascular diseases. However, the low solubility of free phytosterols in water and oil limits their application in food. Studies have shown that phytosterol esters prepared by esterification of phytosterols and fatty acids can greatly increase the fat solubility of phytosterols, and can be easily added to oils or oil-containing foods. In addition, the absorption and utilization rate of phytosterol ester is 5 times that of phytosterol, and it has better lipophilicity and better cholesterol-lowering effect. It is a new type of functional food base material. In 2010, phytosterol esters were listed as new food resources by the Ministry of Health of my country....

AI Technical Summary

Problems solved by technology

In recent years, studies on the preparation of immobilized enzymes using magnetic particles as carriers have emerged in an endless stream. For example, Li Yanfeng et al. have prepared three kinds of magnetically immobilized lipases containing epoxy substrates. The stability of such immobilized enzymes is significantly better than that of free enzymes; however, magnetic The surface modification of materials usually adopts the method of silanization, which requires the use of a large amount of toxic toluene as a solvent, and the preparation process is not environmentally friendly.

CN101280298A adopts Fe with surface adsorbed amino group 3 o 4 The powder is used as a carrier to prepare the immobilized enzyme, and its activity decreases significantly with the increase of the number of repeated uses

However, the research on magnetically immobilized enzymes is mainly focused on the preparation method and preliminary activity evaluation. There are still few magnetically immobilized enzymes that can be used to catalyze actual chemical reactions, and magnetically immobilized enzymes for the production of phytosterol esters have not been reported.