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Process for control synthesis of nanometer mercuric sulfide particles based on sheep-anti-human antibody as soft template

A nanoparticle, human antibody technology, applied in chemical instruments and methods, nanotechnology for materials and surface science, nanotechnology, etc., can solve the problems of harsh experimental conditions, poor dispersion, complex operation, etc. Simple, overcoming complex reactions, good biocompatibility

Inactive Publication Date: 2011-11-23
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the defects of harsh experimental conditions, complex operation, poor dispersion and easy agglomeration in the traditional method of synthesizing mercury sulfide, and to provide a simple preparation method using antibodies as soft templates to control the synthesis of spherical nanoparticles of mercury sulfide

Method used

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  • Process for control synthesis of nanometer mercuric sulfide particles based on sheep-anti-human antibody as soft template
  • Process for control synthesis of nanometer mercuric sulfide particles based on sheep-anti-human antibody as soft template
  • Process for control synthesis of nanometer mercuric sulfide particles based on sheep-anti-human antibody as soft template

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Experimental program
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Embodiment 1

[0020]During the preparation process, add 5 mg goat anti-human antibody to make 150 mL aqueous solution in a 250 mL three-necked bottle, then add 8 mg mercuric acetate to make 50 mL aqueous solution, and stir at a constant speed for 8 hours under the protection of nitrogen , the reaction temperature is normal temperature 20°C. Then add 50 mL of 0.04 mg mL –1 The aqueous solution of thioacetamide immediately changed from colorless to yellow. After stirring for 20 minutes, let it stand at room temperature for 48 hours, use a high-speed centrifuge to centrifuge the product obtained at 18,500 rpm for 30 minutes, and wash it three times with deionized water to obtain pure nanoparticles of mercury sulfide. Store at 4°C for later use.

Embodiment 2

[0022] During the preparation process, add 5 mg goat anti-human antibody to make 150 mL aqueous solution in a 250 mL three-necked bottle, then add 8 mg mercuric acetate to make 50 mL aqueous solution, and stir at a constant speed for 8 hours under the protection of nitrogen , using an ice bath to bring the reaction temperature to 4 °C. Then add 50 mL of 0.04 mg mL –1 The aqueous solution of thioacetamide immediately changed from colorless to yellow. After stirring for 20 minutes, let it stand at room temperature for 48 hours, use a high-speed centrifuge to centrifuge the product obtained at 18,500 rpm for 30 minutes, and wash it three times with deionized water to obtain pure nanoparticles of mercury sulfide. Store at 4°C for later use.

Embodiment 3

[0024] During the preparation process, add 5 mg of goat anti-human antibody to make 150 mL of PBS buffer solution in a 250 mL three-necked bottle, then add 8 mg of mercuric acetate to make 50 mL of aqueous solution, and stir at a constant speed under the protection of nitrogen for 8 Hour, the reaction temperature is normal temperature 20 ℃. Then add 50 mL of 0.04 mg mL –1 The aqueous solution of thioacetamide immediately changed from colorless to yellow. After stirring for 20 minutes, let it stand at room temperature for 48 hours, use a high-speed centrifuge to centrifuge the product obtained at 18,500 rpm for 30 minutes, and wash it three times with deionized water to obtain pure nanoparticles of mercury sulfide. Store at 4°C for later use.

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Abstract

The invention which provides a process for control synthesis of nanometer mercuric sulfide particles based on a sheep-anti-human antibody as a soft template belongs to technical fields of mercuric sulfide synthesis and nanometer materials. The process has many advantages that: firstly, conditions of reaction generation are mild; secondly, the nanometer particles synthesized through a template control method have the characteristics of uniform particle size and good dispersiveness, and insufficients of traditional methods are overcome; in addition, a material synthesized through treating biomolecules as the template has a good biological compatibility, and protein molecules directly coated on surfaces of the material can interact with other biomolecules, so the material can be used in biological tagging, biological imaging analysis and the like, and has an absolute advantage in the biomineralization aspect; finally, the process has a versatility, and other compounds of zinc sulfide, silver sulfide, cadmium sulfide, lead sulfide, copper sulfide and the like can be obtained through control synthesis with the protein molecules as the template. The process of the present invention is avery effective process for control synthesis of nanometer particles.

Description

technical field [0001] The invention relates to a method for preparing nano-particle mercury sulfide, which belongs to the field of synthesis and application of nano-material mercury sulfide, in particular to a method for controlling the synthesis of nano-material mercury sulfide based on a goat anti-human antibody as a soft template. Background technique [0002] In recent years, research on semiconductor nanomaterials has attracted attention from various fields. This is because compared with other materials, semiconductor nanomaterials have certain characteristics in terms of optical, electrical, magnetic and chemical properties (Fan H. J., Knez M., Scholz R., Nielsch K., Pippel E., Hesse D ., Zacharias M., Gösele U. Nat. Mater. 2006, 5(8) :627–631; Liu Z. P., Liang J. B., Xu D., Lu J., Qian Y. T. Chem. Commun. 2004, 23 :2724; Yang L., Xing R. M., Shen Q. M., Jiang K., Ye F., Wang J. Y., Ren Q. S. J. Phys. Chem. B 2006, 110 :10534–10539.). Therefore, it has broad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G13/00B82Y30/00B82Y40/00
Inventor 吕弋刘星宋红杰刘睿蔡苹杨
Owner SICHUAN UNIV
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