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Application of lycorine in preparing medicament for treating diseases caused by human enterovirus 71 type infection

A technology of human enterovirus, lycorine, applied in the field of medicine

Active Publication Date: 2012-12-19
INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] There are no reports of drugs that can effectively inhibit EV71 infection

Method used

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  • Application of lycorine in preparing medicament for treating diseases caused by human enterovirus 71 type infection
  • Application of lycorine in preparing medicament for treating diseases caused by human enterovirus 71 type infection
  • Application of lycorine in preparing medicament for treating diseases caused by human enterovirus 71 type infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Lycorine inhibits the replication of EV71 in cells

[0026] In the EV71-infected human rhabdomyoma cell model, the newly cultured 24-hour human rhabdomyoma cells were inoculated into 96-well plates, and the number of cells per well was 2×10 4 indivual. After 24 hours of cultivation in a 37°C incubator, the virus was inoculated.

[0027] The EV71 used is a popular strain in mainland China, the strain name is FY0805, isolated from Fuyang, Anhui, and the gene sequence is stored in NCBI as HQ882182. The dose of virus inoculated cells was 100TCID 50 , with a volume of 98 microliters, and continue to culture in a 37°C incubator.

[0028] Lycorine hydrochloride was dissolved with physiological saline and administered 2 hours after the virus infected the cells with a drug volume of 2 microliters. The final drug concentrations were 0.1 μg / ml, 0.5 μg / ml, 1 μg / ml, 2 μg / ml, 5 μg / ml, and 10 μg / ml, respectively. Observe the cytopathy within 3 days, and calculate the e...

Embodiment 2

[0032] Example 2: Therapeutic effect of lycorine on EV71-infected mice

[0033]The purpose of this experiment is to verify the therapeutic effect of lycorine on EV71 in mice. The model used is a model of 10-day-old ICR mice infected with the popular EV71 strain in mainland China. The strain is the adapted strain of the clinical isolate FY0805 in mice, the strain name is MP10, and the gene sequence is stored in NCBI as HQ712020. Take 1×10 7 TCID 50 After a dose of MP10 virus was injected intraperitoneally to infect 10-day-old ICR mice, the mice showed symptoms of hindlimb paralysis and weight loss within 4 days, and all died within 10 days after infection.

[0034] Using the above model, the therapeutic effect of lycorine was evaluated in vivo. The mice used were SPF grade ICR mice, 10 days old, half male and half male. First, the mice were infected with the virus by intraperitoneal injection, and the intraperitoneal injection was administered 1 day after the infection (lyc...

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Abstract

The invention belongs to the field of medicaments, discloses application of lycorine, lycorine salt, lycorine hydrate, lycorine optical isomer or lycorine prodrug in preparing medicaments for treating diseases caused by human enterovirus 71 type infection, preferably treating hand-foot-and-mouth disease, herpangina, viral meningitis, viral encephalitis, flaccid paralysis, pulmonary edema and vital myocarditis. In vitro and in vivo tests prove that the lycorine can inhibit replication and lesion of enterovirus (EV) 71 in cells, has excellent function of inhibiting the EV 71 virus, and has clinical application prospect.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of lycorine as a medicine for treating diseases caused by human enterovirus 71 infection. Background technique [0002] Lycorine is a pyrrolophenanthridine type alkaloid with the molecular formula C 16 h 17 NO 4 , which exists in the bulbs of the traditional medicinal plant Lycoris, is a relatively high content of alkaloids, and its structural formula is as follows: [0003] [0004] Lycorine has a more significant emetic effect and less toxicity; it has an expectorant effect. Lycorine is hydrogenated to generate dihydrolycorine, which has a strong anti-amebic dysentery effect and is less toxic, and has been used clinically. The propylamine salt of lycorine exhibited antitumor effects in animals. Biological activity studies have shown that lycorine and its derivatives have anti-inflammatory, anti-malarial, inhibitory acetylcholinesterase, and cardiovascular protect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4745A61P29/00A61P31/14A61P25/00A61P9/00A61P11/00A61P1/00
Inventor 张连峰杨亚军刘江宁秦川马春梅
Owner INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI
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