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Application of extract of Chinese angelica and szechuan lovage rhizome composition to preparation of medicament for promoting ovary granular cell proliferation

A technology of granulosa cells and compositions, which is applied in the field of preparation of drugs for promoting the proliferation of ovarian granulosa cells, the extract of angelica and chuanxiong, which can solve the problems of no ovarian granulosa cell proliferation, achieve small side effects, clear active ingredients, The effect of convenience

Inactive Publication Date: 2011-04-27
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, Angelica and Rhizoma Chuanxiong are mainly used to treat dizziness, headache and dystocia caused by excessive blood loss. There are no research reports on treating ovarian dysfunction and promoting the proliferation of ovarian granulosa cells.

Method used

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  • Application of extract of Chinese angelica and szechuan lovage rhizome composition to preparation of medicament for promoting ovary granular cell proliferation
  • Application of extract of Chinese angelica and szechuan lovage rhizome composition to preparation of medicament for promoting ovary granular cell proliferation
  • Application of extract of Chinese angelica and szechuan lovage rhizome composition to preparation of medicament for promoting ovary granular cell proliferation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Effect of the Extract of Angelica and Rhizoma Chuanxiong Composition on the Proliferation of Ovarian Granulosa Cells

[0020] 1. Experimental materials

[0021] 1.1 Main Instruments

[0022] SpectrumMAX190 automatic microplate reader (Molecular Devices, USA), XSZ-D 2 Optical inverted microscope (Chongqing Optical Instrument Factory), NU-4950CO 2 Incubator (NuAire, USA), Z323 centrifuge (Hermile, Germany). Waters 2695 high-performance liquid chromatograph (U.S. waters company), 2996PDA diode array detector (U.S. waters company), automatic sampler; Empower chromatographic workstation (U.S. waters company). KQ-250E ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.), EPED ultrapure water system.

[0023] 1.2 Experimental animals

[0024] 21-day-old clean healthy female SD rats, weighing 60-70 g, were purchased from the Experimental Animal Center of Nanjing Medical University, animal license number: SCXK (Su) 2008-0004.

[0025] 2. Experimental meth...

Embodiment 2

[0040] Determination of the dissolution rate of chlorogenic acid, ferulic acid, ligusticolide I, ligustilide H, ligustilide, n-butenyl ligusticolide and other components after the compatibility of angelica and Chuanxiong.

[0041] 1. Investigation of content determination methodology

[0042] 1.1 Chromatographic conditions

[0043] Chromatographic column: Alltima-C18 (250×4.6mm, 5μm); mobile phase: acetonitrile (B)-0.2% formic acid aqueous solution (A), system gradient elution mobile phase. PAD detector wavelength scanning range: 210-400nm. Column temperature: 30°C; injection volume: 10 μL. Components are detected at the wavelength of maximum absorption.

[0044] 1.2 Preparation of mixed reference substance

[0045] Accurately weigh the reference substance chlorogenic acid, ferulic acid, ligustilide I, listonin H, ligustilide, and n-butenyl ligustilide and dilute the mixed standard solution solution to obtain the product.

[0046] 1.3 Preparation of the test solution

[...

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Abstract

The invention discloses new application of an extract of Chinese angelica and szechuan lovage rhizome composition to the preparation of a medicament for promoting ovary granular cell proliferation. Intensive study is performed on a large quantity of ancient and modern prescriptions and traditional Chinese medicine application data, traditional Chinese medicine resources are fully utilized according to a traditional Chinese medicine theory and the pathogenesis of ovarian functional disturbance and insufficient growth of ovary granular cells and a large number of pharmacological tests are performed based on the current researches of Chinese angelica and szechuan lovage rhizome, so that new application of the Chinese angelica and szechuan lovage rhizome to the treatment of ovarian functional disturbance and the promotion of ovary granular cell proliferation; moreover, the weight ratio of the Chinese angelica and szechuan lovage rhizome composition with the highest activity is screened through a large quantity of experiments. When clinically taken as a medicament for treating ovarian functional disturbance and promoting ovary granular cell proliferation, the extract has the advantages of good curative effect, definite active ingredient, small side effect, convenience of taking and the like.

Description

technical field [0001] The invention relates to a traditional Chinese medicine composition of angelica and chuanxiong, in particular to a new application of the extract of the composition of angelica and chuanxiong in preparing a medicine for promoting the proliferation of ovarian granulosa cells. Background technique [0002] The physiological functions of the ovary include ovulation and endocrine functions, which are of great significance to women's reproductive function, physical and mental health and quality of life. Ovarian dysfunction can lead to ovulation dysfunction, abnormal corpus luteum function and endocrine disorders, etc., which can cause a variety of gynecological diseases, such as uterine dysplasia, infertility, premature ovarian failure, perimenopausal syndrome, etc., and can affect other organs throughout the body. The physiological function of the organ. Ovarian granulosa cells are the main functional cells of the ovary, and their proliferation and differ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/236A61P15/00
Inventor 唐于平华永庆李伟霞禹良艳钱大玮段金廒
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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