Cis-platinum lung cancer-resistant active targeting stealth liposomes and preparation method thereof
An active targeting, anti-lung cancer technology, applied in the direction of liposome delivery, anti-tumor drugs, heavy metal active ingredients, etc., can solve the problems of low activity, unstable nature, and easy to produce drug resistance
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[0025] The preparation process of the present invention not only can contain polymer-antibody F(ab') 2 The buffer solution of the fragment conjugate solution is mixed with the organic phase containing the prescription amount of cisplatin and the fat-soluble membrane material, and the stealth lipoid body with the anti-lung cancer monoclonal antibody fragment connected on the surface is prepared through the self-assembly process, which can also be obtained by prescription Adjustment, the same preparation process was used to prepare cisplatin-like liposomes with different charges on the surface, cisplatin-stealth liposomes with different proteins on the surface, and cisplatin-like liposomes and cisplatin-stealth liposomes .
[0026] Under the premise that the main prescription ingredients and dosage are basically unchanged, the individual prescription ingredients and ratios are adjusted to obtain ordinary liposomes with no charge on the surface, positively charged liposomes, and ...
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[0032] 1 polymer-anti-cancer cell monoclonal antibody F(ab') 2 Preparation of Fragment Modifier (A):
[0033] Preparation, separation, purification and identification methods of the anti-lung cancer monoclonal antibody and its Fab fragment: according to conventional methods. Human lung squamous cell carcinoma (Yunnan silicon lung cancer, YTMLC-90) cell line was prepared and purified according to conventional monoclonal antibody, followed by enzyme digestion. The test group (YTMLC-90), small cell lung cancer (NCL-H446), lung adenocarcinoma (A 2 ) and the control group (human bronchial epithelial cells HBE), were confirmed by immunohistochemistry and Western-blot tests, and have lung cancer targeting. In order to avoid modifying the surface of liposomes, the monoclonal antibody as a heterologous protein has an Fc fragment, which is discovered by specific proteins in the plasma and cleared by the endothelial reticulum system, and also avoids secondary activation of the bo...
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