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Extracts of panaxtriol saponins and preparation process thereof

A technology of notoginseng saponin and notoginseng triol group, which is applied in the field of extract notoginseng triol saponin extract of traditional Chinese medicine notoginseng and its preparation field, which can solve the problems of poor separation effect and achieve the reduction of hepatic microcirculation disturbance, The preparation method has the advantages of simple process and convenient operation

Inactive Publication Date: 2011-02-09
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above methods all use macroporous adsorption resin for separation, and the separation effect is not good. The inventors used C 18 Reversed-phase silica gel column separation, a new group of notoginsengtriol saponin extracts and their preparation methods were tested. These notoginsengtriol saponin extracts have high curative effect, high purity, good absorption, stable quality, and the separation effect of the preparation method Good, high content, simple process, convenient operation, low cost, suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 10

[0062] The preparation of embodiment 1 notoginseng triol group saponins

[0063] Take notoginseng, crush it into coarse particles, add 6 times the amount of 70% ethanol to reflux and extract twice, the first time is 2.5 hours, and the second time is 2 hours to combine the extracts, and the extracts are concentrated under reduced pressure below 70°C to 1.5 times the medicinal materials , centrifuged at 4200r / min for 20min, the supernatant was loaded with D941 resin (resin and medicinal material 0.5~1:1g / g) at a flow rate of 0.8BV / h, 10 times the column volume, with a flow rate of 1BV / h, washed with water; liquid and water wash. Concentrate to dryness under reduced pressure, dissolve the dry paste with mobile phase, and use a particle size of 30-60 μmC 18 Reversed-phase silica gel packing column, for separation, sample and separation with C 18 Reverse phase silica gel ratio 1:200, wash with acetonitrile and water (23:77), thin layer chromatography (thin layer conditions: chlor...

Embodiment 3

[0077] Take notoginseng, crush it into coarse particles, add 6 times the amount of 70% ethanol to reflux and extract twice, the first time is 2.5 hours, and the second time is 2 hours to combine the extracts, and the extracts are concentrated under reduced pressure below 70°C to 1.5 times the medicinal materials , 300 mesh filter cloth, supernatant D941 resin (resin and medicinal materials 0.5 ~ 1: 1g / g) sample flow rate is 1BV / h, 8 times the column volume, flow rate 1.5BV / h, washed with water; liquid and water wash. Concentrate to dryness under reduced pressure, dissolve the dry paste with mobile phase, and use a particle size of 30-60 μmC 18 Reversed-phase silica gel packing column, for separation, sample and separation with C 18 The ratio of reversed-phase silica gel was 1:100, washed with acetonitrile and water (25:75), and the elution endpoint was judged by peak collection with an online ultraviolet detector. Collect according to the peak, and recover the organic solven...

Embodiment 4

[0079] The extraction steps are as follows:

[0080] a. take Radix Notoginseng, and crush it into coarse particles;

[0081] b. Add 4 times the amount of 45% ethanol to decoct twice, each time for 1 hour;

[0082] c. Combine the decoctions and concentrate under reduced pressure below 70°C to 1.5 times the volume of the medicinal materials;

[0083] d. Stand still to room temperature, centrifuge at 3500rpm for 20min, and separate the supernatant;

[0084] e. Put D941 resin on the supernatant, resin and medicinal material 0.5:1g / g, sample loading flow rate is 0.5BV / h, 5 times column volume, flow rate 1BV / h, wash with water; collect sample loading effluent and water washing solution.

[0085] f. Concentrate the sample loading effluent and washing solution under reduced pressure to dryness below 70°C;

[0086] g. The above dry paste is dissolved in the mobile phase and then applied to C 18 Reverse Phase Silica Column, C 18 Reverse filler particle size 30μm;

[0087] h. Eluti...

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Abstract

The invention relates to extracts of traditional Chinese medicines and preparation thereof, in particular to extracts of panaxtriol saponins, extracts of panax notoginseng and preparation thereof. The preparation process comprises the steps of extraction, separation, concentration and the like.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract and its preparation, in particular to a notoginseng triol saponin extract and its preparation. Background technique [0002] Panax notoginseng (Panax notoginseng, PN) is the air-dried root of the traditional Chinese medicine Panax notoginseng (Burk) F.H.Chen. Panax notoginsenosides (also known as Panax notoginsenosides, English name: Panax Notoginsenosides, PNS) is the main active ingredient of Panax notoginseng, composed of more than 30 different saponins, widely used clinically to treat diseases related to microcirculation disorders , such as cardiovascular disease and liver dysfunction. [0003] Notoginseng saponins have been included in the national pharmacopoeia, and corresponding preparations are on the market, such as Xuesaitong for injection. The main components of notoginsenosides are: ginsenoside Rb1, ginsenoside Rg1, and notoginsenoside R1. There are many reports on their prep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258A61P1/16A61P9/00
Inventor 李瑞明张兰兰王玉
Owner TIANJIN TASLY PHARMA CO LTD
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