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Method for preparing bionic modified collagen tissue repair material

A collagen tissue and repair material technology, applied in tissue regeneration, coating, medical science, etc., can solve the problems of lower material quality, repair effect, poor biocompatibility, etc., achieve low cost, facilitate large-scale production, and simple molding process effective effect

Inactive Publication Date: 2011-01-26
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this can prolong the degradation time of the material, due to the poor biocompatibility of non-collagen materials, it will inevitably reduce the quality and repair effect of the entire material.

Method used

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  • Method for preparing bionic modified collagen tissue repair material
  • Method for preparing bionic modified collagen tissue repair material
  • Method for preparing bionic modified collagen tissue repair material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation method of a biomimetic modified collagen tissue repair material, the steps are as follows:

[0030] (1) Prepare a collagen-acetic acid solution of 7mg / ml with a 3% volume fraction of acetic acid for type I collagen;

[0031] (2) Cast the collagen-acetic acid solution in step (1) into a plastic mold, pre-freeze at -60°C for one day (24 hours), and freeze-dry at -80°C in vacuum to obtain a collagen sponge of 55mm×15mm×12mm;

[0032] (3) Place the freeze-dried collagen sponge in step (2) in a vacuum drying oven, and conduct a vacuum thermal crosslinking reaction at 120°C for 24 hours. After stopping heating, keep the vacuum state and cool to room temperature before taking out the sample;

[0033] (4) The collagen sponge after thermal cross-linking is soaked in a mixed solution of EDC and NHS at 4° C. for 12 hours to promote further chemical cross-linking. The concentration of EDC in this mixed solution is 14mM, the concentration of NHS is 5.6mM, and the molar ratio...

Embodiment 2

[0039] The preparation method of a biomimetic modified collagen tissue repair material, the steps are as follows:

[0040] (1) Prepare type I collagen with 3% acetic acid solution to make 5mg / ml collagen solution;

[0041] (2) Cast the collagen-acetic acid solution in step (1) into a plastic mold, pre-freeze at -60°C for one day, and freeze-dry in vacuum at -80°C for 48 hours to obtain a collagen sponge with a size of 55mm×15mm×15mm;

[0042] (3) Place the freeze-dried collagen sponge in step (2) in a vacuum drying oven, and conduct a vacuum thermal cross-linking reaction at 100° C. for 24 hours. After stopping heating, keep the vacuum state and cool to room temperature before taking out the sample;

[0043] (4) The thermally crosslinked collagen sponge is soaked in a mixed solution of EDC and NHS at 4°C for 12 hours. The concentration of EDC is 12mM and the concentration of NHS is 4.8mM. After cross-linking, discard the EDC-NHS solution, rinse with deionization 10 times, pre-freeze...

Embodiment 3

[0048] The preparation method of a biomimetic modified collagen tissue repair material, the steps are as follows:

[0049] (1) Prepare type I collagen with a 3% acetic acid solution to prepare a 6mg / ml collagen-acetic acid solution, and add bioactive glass powder, where the mass ratio of bioactive glass to collagen is 1:4;

[0050] (2) Cast the collagen-acetic acid solution in step (1) into a plastic mold, pre-freeze at -60°C for one day, and freeze-dry in vacuum at -80°C to obtain a collagen sponge of 55mm×15mm×12mm;

[0051] (3) Place the freeze-dried collagen sponge in step (2) in a vacuum drying oven, heat the crosslinking reaction in a vacuum at 110°C for 24 hours, stop heating and then keep the vacuum state and cool to room temperature before taking out the sample;

[0052] (4) The collagen sponge after thermal crosslinking is soaked in a 0.25% (mass fraction) glutaraldehyde solution at 4°C for 4 hours. After cross-linking, discard the glutaraldehyde solution, rinse with deioniz...

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Abstract

The invention discloses a method for preparing a bionic modified collagen tissue repair material. In the preparation method, the tissue repair material is prepared from type I collagen serving as a raw material, is formed by the processes of freeze-drying, pressing, coating, cross-linking, drying and the like and has a bionic structure, namely the structure of a compact layer and a loose layer is in graded distribution and does not have any obvious interface layer. The bionic modified collagen tissue repair material does not contain any chemical components, has high biocompatibility and can be completely absorbed by organisms, wherein the compact layer has barrier function and can effectively prevent cells at the periphery of defective tissues from growing into a damaged tissue area; and the loose layer can guide the adhesion and proliferation of the cells of the defective tissues so as to fulfill the aim of tissue repair.

Description

Technical field [0001] The invention relates to a biomedical collagen tissue repair material, in particular to a preparation method of a collagen-based tissue repair material with a dense layer and a loose layer bionic structure. The invention can be applied to repair various local hard and soft tissue defects caused by tissue necrosis or tumor resection. technical background [0002] As a natural polymer material, collagen has received more and more attention from experts and scholars at home and abroad in the field of guiding tissue repair and regeneration. This is mainly because collagen is the main component of extracellular matrix, has good biocompatibility, and can promote cell adhesion, proliferation and differentiation. In some applications, collagen tissue repair materials must not only successfully guide cell growth, but more importantly, serve as a physical barrier to provide a stable space for the growth of new tissues while preventing the growth of surrounding tissu...

Claims

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Application Information

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IPC IPC(8): A61L31/12C08L89/00C08J9/28C08J7/04C08J3/24
CPCA61L27/56A61L27/446C08H1/06A61L27/46A61L2430/00A61L27/24
Inventor 任力王迎军田小俊林绣丹
Owner SOUTH CHINA UNIV OF TECH
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