Method for identifying cordyceps sinensis phorozoon by using immunohistochemistry

Abstract

The invention relates to a method for identifying phorozoon of cordyceps sinensis by using immunohistochemistry. The method comprises the following steps of: preparing and staining hypha antibodies, and comparing staining results; directly observing a staining picture, wherein positive staining (stained with color) means cordyceps sinensis, and negative staining (not stained with color) means not phorozoon of the cordyceps sinensis. The method creatively adopts hyphae of cordyceps militaris with highest cordyceps sinensis homology to absorb polyvalent serum and has the characteristic of high specificity. The identification method has the advantages of no need of instrument, simpleness, convenience, easy popularization, short staining time, higher speedability than that of the conventional identification method and the molecular biology method, and low cost and can be used for identifying the phorozoon of the cordyceps sinensis hyphae.

Description

technical field [0001] The present invention relates to an identification method of Cordyceps sinensis, more specifically to an immunohistochemical identification method. Background technique [0002] I. Cordyceps sinensis (cordyceps sinensis) is an ascomycete, which has conidia stage (asexual type) and ascospore stage (sexual type) in its life history. The Cordyceps sinensis strains used in actual production such as artificial cultivation and liquid fermentation are all asexual stages. Domestic scholars have conducted extensive research on the isolation and cultivation of the anamorph of Cordyceps sinensis. Different researchers have isolated different strains, and they all claim to be the anamorph of Cordyceps sinensis. However, there are several asexual types of Cordyceps sinensis? What is the true asexual form of Cordyceps sinensis? This poses a challenge to the traditional classification method based on morphological structure, and the establishment of molecular biol...

AI Technical Summary

Problems solved by technology

Although these methods are simple and easy to operate, they have the disadvantages of long identification time and cannot completely rule out the possibility of alternate, associated, and mixed infection of other strains, that is, poor specificity; the latter includes rDNA sequencing and random amplified polymorphic DNA ( RAPD) etc., although these methods have strong specificity, they need expensive instruments and medicines, the cost is high, and it is not easy to popularize and apply

[0025] The present invention has overcome the defect that above-mentioned prior art exists as: 1. traditional identification method does not have specificity; Operation skills that can only be mastered by professionals; 4. Traditional identification methods take a long time to complete an identification and the cost is high; 3. Molecular biological identification methods require sterile conditions, and are all micro-operations, and must be completed by specialized technical training personnel Operation; 5. Molecular biological method identification From DNA extraction, to PCR amplification and gene sequence determination, there are also a series of defects such as long time, expensive equipment and high-priced drugs

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