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Biological weed control compound and preparation method thereof

A technology of herbicides and compounding agents, which is applied in the fields of botanical equipment and methods, biocides, herbicides and algicides, can solve the problems of unsatisfactory manual removal and mechanical control, difficult chemical control, etc. Cost of biological control, good weed control effect, and strong host-specific effect

Active Publication Date: 2010-10-13
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above methods have certain effects, due to the strong reproductive ability and stress resistance of the grass, manual removal and mechanical control are not ideal, and chemical control is also relatively difficult.
[0007] In terms of biological control research on crabgrass weeds, the inventor found that conidia and hyphae of a fungal strain of Curvularia can be used for weed control (patent No. CN01134002. Further research is needed on grass

Method used

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  • Biological weed control compound and preparation method thereof
  • Biological weed control compound and preparation method thereof
  • Biological weed control compound and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Conidia culture: Cultivate QZ-2000 in a culture medium containing potato, glucose and agar for 7 days; take a small piece of colony and transfer it to wheat bran-sawdust or wheat bran solid medium Cultivate for 7 days, cut the bacteria into small pieces and lay them flat in a flat container with a thickness of 1cm, place them under black light for 48 hours, air-dry and pulverize them, and then sieve to get spore powder; or inoculate the small pieces of colonies on corn Shake culture in powder-soybean powder-sucrose liquid medium for 6 days, filter out the residual liquid of the medium, rinse with sterile water to obtain mycelium, and obtain a suspension of mycelium fragments after crushing, and put it in a petri dish under light 36h, the spores were obtained;

[0041] (2) Configure chemical herbicide mother liquor: prepare the mother liquor of the chemical herbicide nicosulfuron-methyl preparation diluted 100 times, take 1ml of nicosulfuron-methyl preparation and ad...

Embodiment 2

[0044] (1) Conidia culture: Cultivate QZ-2000 in a culture medium containing potato, glucose and agar for 7 days; take a small piece of colony and transfer it to wheat bran-sawdust or wheat bran solid medium Cultivate for 7 days, cut the bacteria into small pieces and lay them flat in a flat container with a thickness of 1cm, place them under black light for 48 hours, air-dry and pulverize them, and then sieve to get spore powder; or inoculate the small pieces of colonies on corn Shake culture in powder-soybean powder-sucrose liquid medium for 6 days, filter out the residual liquid of the medium, rinse with sterile water to obtain mycelium, and obtain a suspension of mycelium fragments after crushing, and put it in a petri dish under light 36h, the spores were obtained;

[0045] (2) Prepare chemical herbicide mother liquor: prepare the mother liquor of the chemical herbicide oxyfluorfen preparation diluted 100 times, take 1ml of the oxyfluorfen preparation and add it to 100ml ...

Embodiment 3

[0048] (1) Conidia culture: Cultivate QZ-2000 in a culture medium containing potato, glucose and agar for 7 days; take a small piece of colony and transfer it to wheat bran-sawdust or wheat bran solid medium Cultivate for 7 days, cut the bacteria into small pieces and lay them flat in a flat container with a thickness of 1cm, place them under black light for 48 hours, air-dry and pulverize them, and then sieve to get spore powder; or inoculate the small pieces of colonies on corn Shake culture in powder-soybean powder-sucrose liquid medium for 6 days, filter out the residual liquid of the medium, rinse with sterile water to obtain mycelium, and obtain a suspension of mycelium fragments after crushing, and put it in a petri dish under light 36h, the spores were obtained;

[0049] (2) Prepare chemical herbicide mother liquor: prepare the mother liquor diluted 100 times of the chemical herbicide butachlor-oxadiazone preparation, take 1ml of oxyfluorfen preparation and add it to 1...

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Abstract

The invention discloses a weed control compound and a preparation method thereof. The weed control compound is mainly composed of conidium of Curvularia fungus strain QZ-2000 with preservation number CGMCC NO.0640 and a chemical herbicide, wherein the concentration of the conidium is 1-10g / L, and the concentration of the chemical herbicide is 1 / 20-1 / 3 of a conventional dosage. The invention adopts that the conidium is compounded with the chemical herbicide; on one hand, conidium dosage is lowered, biological prevention and cure cost is lowered, and the chemical herbicide performs the synergy when compound compared with the situation that only spore is adopted; on the other hand, lowers pollution of chemical herbicide is lowered.

Description

technical field [0001] The invention relates to a compound herbicide, in particular to a compound composed of spores of a strain of Campylobacter and a chemical herbicide. Background technique [0002] Weeds have always been considered as one of the biological disasters that seriously affect agriculture and the environment. Chemical herbicides have been used effectively for the control of weeds in agricultural fields for more than a century. However, due to the large-scale application of chemical herbicides, many serious problems such as the formation of resistant weeds, the decline of soil and water quality, herbicide residues, water pollution and the emergence of resistant (tolerant) weeds are becoming more and more serious. Moreover, the extensive development and use of chemical herbicides has not completely solved the problem of weed damage. With the improvement of global environmental protection awareness, the research on biological herbicides with high herbicidal act...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N47/36A01N33/22A01N43/824A01N37/26A01P13/00
Inventor 强胜陆艳娜朱云枝高同军徐春凤陈世国
Owner NANJING AGRICULTURAL UNIVERSITY
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