Insect-borne infectious disease pathogen detection kit and application thereof
A kit and infectious technology, applied in the detection kit and application field of insect-borne infectious disease pathogens, can solve the problems of low detection efficiency and trouble, achieve high sensitivity, improve detection efficiency, and reduce workload
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[0125] Plasma samples from 20 volunteers in a hospital (one of the volunteers are known to be positive patients and the other volunteers are negative) were subjected to multiplex PCR testing after nucleic acid extraction.
[0126] 1. Sample processing:
[0127] Take 200 microliters of plasma samples, and directly extract nucleic acids with nucleic acid extraction reagents.
[0128] 2. Sample loading and detection:
[0129] 1) Reagent preparation: Take out the nucleic acid detection reagent and dissolve it at room temperature (freeze-thaw);
[0130] 2) Reagent preparation: Prepare a fluorescent PCR reaction system according to the number of samples + 2;
[0131] Both PCR reaction systems 1 and 2 were prepared according to this number of tubes;
[0132] 3) Adding samples: Take 20 microliters of nucleic acid samples, add them to 30 microliters of PCR reaction system, close the cap tightly, mix well, centrifuge briefly, and put on the machine.
[0133] 4) Set the amplification...
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