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Insect-borne infectious disease pathogen detection kit and application thereof

A kit and infectious technology, applied in the detection kit and application field of insect-borne infectious disease pathogens, can solve the problems of low detection efficiency and trouble, achieve high sensitivity, improve detection efficiency, and reduce workload

Pending Publication Date: 2022-05-17
赵俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, kits for the detection of a single type of virus have been developed, but this single detection method is cumbersome and the detection efficiency is low

Method used

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  • Insect-borne infectious disease pathogen detection kit and application thereof
  • Insect-borne infectious disease pathogen detection kit and application thereof
  • Insect-borne infectious disease pathogen detection kit and application thereof

Examples

Experimental program
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Effect test

specific Embodiment

[0125] Plasma samples from 20 volunteers in a hospital (one of the volunteers are known to be positive patients and the other volunteers are negative) were subjected to multiplex PCR testing after nucleic acid extraction.

[0126] 1. Sample processing:

[0127] Take 200 microliters of plasma samples, and directly extract nucleic acids with nucleic acid extraction reagents.

[0128] 2. Sample loading and detection:

[0129] 1) Reagent preparation: Take out the nucleic acid detection reagent and dissolve it at room temperature (freeze-thaw);

[0130] 2) Reagent preparation: Prepare a fluorescent PCR reaction system according to the number of samples + 2;

[0131] Both PCR reaction systems 1 and 2 were prepared according to this number of tubes;

[0132] 3) Adding samples: Take 20 microliters of nucleic acid samples, add them to 30 microliters of PCR reaction system, close the cap tightly, mix well, centrifuge briefly, and put on the machine.

[0133] 4) Set the amplification...

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Abstract

The invention discloses an insect-borne infectious disease pathogen detection kit and application, the insect-borne infectious disease pathogen detection kit can detect dengue virus, Zika virus, yellow fever virus, chikungunya virus, west nile virus and plasmodium in a sample, and is helpful for diagnosis and treatment of insect-borne infectious diseases. By adopting the detection kit disclosed by the invention, various viruses can be identified at one time, so that the workload is greatly reduced, and the detection efficiency is improved.

Description

technical field [0001] The invention specifically relates to an insect-borne infectious disease pathogen detection kit and its application. Background technique [0002] According to WHO statistics, vector-borne diseases account for more than 17% of all infectious diseases and cause more than 700,000 deaths every year. These diseases can be caused by parasites, bacteria, or viruses. Vector-borne viral diseases include malaria, dengue, chikungunya, Zika, yellow fever, West Nile fever, Japanese encephalitis (both transmitted by mosquitoes), tick-borne encephalitis (transmitted by ticks )Wait. [0003] Malaria is a parasitic infection transmitted by mosquitoes and most deaths occur in children under the age of five. Dengue fever is the most prevalent viral infection transmitted by the Aedes mosquito. The burden of these diseases is highest in tropical and subtropical regions. Since 2014, major outbreaks of dengue, malaria, chikungunya, yellow fever and Zika have affected p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/686C12N15/11
CPCC12Q1/701C12Q1/686C12Q2600/16C12Q2600/166C12Q2537/143C12Q2545/101C12Q2545/113Y02A50/30
Inventor 赵俊
Owner 赵俊
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