Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Genetic therapy breast cancer drug preparation method based on microfluidic chip

A microfluidic chip and gene therapy technology, applied in gene therapy, biochemical equipment and methods, stress-stimulated microbial growth methods, etc., can solve the problems of lack of efficient targeting vectors, transfection cytotoxicity, liver cell damage, etc.

Inactive Publication Date: 2010-06-23
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if this kind of DNA computer is to be further tested in vivo, there are still the following shortcomings: 1. All the biochemical reactions of the computer are carried out in test tubes. If the DNA computer needs to enter the body to perform functions, it must be carried by an effective carrier. DNA molecules and enzymes deliver them to the corresponding targets to prevent DNA molecules from being degraded before calculation. Obviously, test tubes cannot be such an effective carrier; And each calculation of a gene needs to introduce a variety of high-concentration exogenous molecules, which may affect the normal physiological functions of the cells; 3. The computer must enter the cell to perform its functions, which increases the difficulty of operation ; 4, the most important point, this computer uses restriction endonuclease (Fok I enzyme) to carry out enzymatic digestion reaction, restriction endonuclease only exists in lower organisms (such as bacteria, virus etc.), can be in specific position Dots can cut double-stranded DNA molecules and thus have the potential to affect the genomes of higher organisms
But it also has its disadvantages: the therapeutic gene is a random non-specific transfection virus antigen can induce the body to produce an immune response, which limits repeated use; recombinant adenovirus can cause transfection cell toxicity and cell death, which limits the time of gene expression; damage to cells
[0016] There are currently problems in breast cancer gene therapy: (1) lack of efficient and specific targeting vectors: it is required to reduce the potential tumorigenicity of viral vectors without damaging normal cells
(2) Controllability of gene expression: current research tends to use tissue and tumor-specific gene promoters to control target gene expression

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Genetic therapy breast cancer drug preparation method based on microfluidic chip
  • Genetic therapy breast cancer drug preparation method based on microfluidic chip
  • Genetic therapy breast cancer drug preparation method based on microfluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Preparation method of drugs for gene therapy of breast cancer based on microfluidic chip: We calculated the expression increase and decrease of two genes most closely related to breast cancer, namely proto-oncogene C-erbB-2 and tumor suppressor gene nm23 situation, and when the expression of both meets the diagnostic criteria, the complete suicide gene HSV-tk is synthesized as an anticancer drug and released.

[0083] In in vitro experiments, we used synthetic shorter DNA molecules to replace longer proto-oncogenes and tumor suppressor genes. Corresponding to proto-oncogene is computational molecule 1 and drug fragment A, and corresponding to tumor suppressor gene is computational molecule 2 and drug fragment B. The incomplete suicide gene molecule is replaced by template strands complementary to drug fragments A and B. Drug fragments A and B can combine with the template strand to form a hybrid double strand, and are connected into a complete double strand under the a...

Embodiment 2

[0093] The content of this embodiment is basically the same as that of Embodiment 1, and its difference mainly lies in:

[0094] 1) We target at least one of the following breast cancer-related proto-oncogenes (C-erbB-2, EGFR, c-myc, ras, int-2, bcl-2, BAG-1, BCSG-2, survivin) One and at least one of the tumor suppressor genes (P53, nm23, PTEN, Rb, P16, P21, CHEK2, BRCA1, BRCA2) related to breast cancer are used as the design basis for the design of drug fragment molecules.

[0095] 2) We design an incomplete suicide gene molecule with a gap for the drug fragment molecule selected in 1), specifically a double-stranded DNA molecule with a gap of the same sequence corresponding to the selected drug fragment molecule.

[0096] 3) The calculation unit and calculation channel of the microfluidic chip as a carrier are selected according to the specific conditions of the above 1) and 2) processes, and the test parameters are also matched with them.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a genetic therapy breast cancer drug preparation method based on microfluidic chip, which includes that: the method adopts biological molecules as computation medium to compute the cancer-related gene expression through biochemical reaction; when the result meets the requirement, the anti-cancer drug is automatically synthesized and released in targeted manner; the anti-cancer drug is complete suicide gene; the biological molecules used as computation medium are DNA molecules and / or enzyme; the oncogene related to breast cancer includes: C-erbB-2, EGFR, c-myc, ras, int-2, bcl-2, BAG-1, BCSG-2 and survivin; the cancer suppressor gene related to breast cancer includes P53, nm23, PTEN, Rb, P16, P21, CHEK2, BRCA1 and BRCA2. The invention can synthesize and release anti-cancer drugs for breast cancer on specified conditions; and the anti-cancer drugs are complete suicide gene. The method can be combined with the nanometer technology and micromachining technology to help improve the treatment effect.

Description

technical field [0001] The invention relates to computer science, molecular biology, medicine and microfluidic chip technology. In particular, it provides a method for preparing a medicine for gene therapy of breast cancer based on a microfluidic chip. Background technique [0002] Cancer is a genetic disease, and diagnosis and treatment at the genetic level are the most basic and accurate methods. Cell carcinogenesis is a process based on the activation of multiple proto-oncogenes and the inactivation of tumor suppressor genes. Therefore, in genetic diagnosis, it is often necessary to make a comprehensive judgment on the expression levels of multiple genes. At present, the diagnosis of cancer is often carried out in vitro, and it is far from reaching the single-cell level by judging the results of blood and tissue tests. Moreover, in vitro diagnosis and in vivo treatment are two relatively independent links, and anticancer drugs often cause greater damage to normal cells....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P35/00A61P15/14C12Q1/68C12M1/42G06N3/12
Inventor 秦建华张宇于浩林炳承
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products