Genetic therapy breast cancer drug preparation method based on microfluidic chip
A microfluidic chip and gene therapy technology, applied in gene therapy, biochemical equipment and methods, stress-stimulated microbial growth methods, etc., can solve the problems of lack of efficient targeting vectors, transfection cytotoxicity, liver cell damage, etc.
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Embodiment 1
[0082] Preparation method of drugs for gene therapy of breast cancer based on microfluidic chip: We calculated the expression increase and decrease of two genes most closely related to breast cancer, namely proto-oncogene C-erbB-2 and tumor suppressor gene nm23 situation, and when the expression of both meets the diagnostic criteria, the complete suicide gene HSV-tk is synthesized as an anticancer drug and released.
[0083] In in vitro experiments, we used synthetic shorter DNA molecules to replace longer proto-oncogenes and tumor suppressor genes. Corresponding to proto-oncogene is computational molecule 1 and drug fragment A, and corresponding to tumor suppressor gene is computational molecule 2 and drug fragment B. The incomplete suicide gene molecule is replaced by template strands complementary to drug fragments A and B. Drug fragments A and B can combine with the template strand to form a hybrid double strand, and are connected into a complete double strand under the a...
Embodiment 2
[0093] The content of this embodiment is basically the same as that of Embodiment 1, and its difference mainly lies in:
[0094] 1) We target at least one of the following breast cancer-related proto-oncogenes (C-erbB-2, EGFR, c-myc, ras, int-2, bcl-2, BAG-1, BCSG-2, survivin) One and at least one of the tumor suppressor genes (P53, nm23, PTEN, Rb, P16, P21, CHEK2, BRCA1, BRCA2) related to breast cancer are used as the design basis for the design of drug fragment molecules.
[0095] 2) We design an incomplete suicide gene molecule with a gap for the drug fragment molecule selected in 1), specifically a double-stranded DNA molecule with a gap of the same sequence corresponding to the selected drug fragment molecule.
[0096] 3) The calculation unit and calculation channel of the microfluidic chip as a carrier are selected according to the specific conditions of the above 1) and 2) processes, and the test parameters are also matched with them.
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