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Application of sodium butyl

A technology of sodium butyrate and minimum inhibitory concentration, used in the field of feed mildew inhibitor and mildew inhibitor, can solve the problems of good light resistance and heat resistance, low effective use concentration, unclear inhibitory concentration, etc.

Inactive Publication Date: 2010-03-24
胡金来
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Good light resistance and heat resistance, less affected by pH, low effective use concentration, and stable aqueous solution, will not be thermally decomposed during processing, or volatilize with water vapor, but it is not clear how different kinds of sodium butylate are under different conditions The inhibitory concentration of mold hinders the application of sodium butylate as an antifungal agent in feed production

Method used

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  • Application of sodium butyl

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 Sodium butyrate measures the minimum inhibitory solubility (MIC) of mold in feed

[0024] 1.1 Preparation of mold spore suspension

[0025] Weigh 25g of chicken feed with mold, which is purchased from the Feed Processing Laboratory of Nanjing Agricultural University, Animal Science and Technology College, put into a conical flask containing 250ml of sterilized normal saline, vibrate on a shaker for 30min, and use a 10ml pipette to Dilute 10ml of the above-mentioned physiological saline with mold at 1:10 in a 25ml test tube, and blow and suck repeatedly 50 times with an ear wash ball and a 1ml pipette to disperse the mold spores. Take 1ml of the dilution with mold and add 9ml of physiological saline, and mix thoroughly to obtain a dilution of 1:100 with mold. Take 1ml of the 1:100 dilution with mold, add it to 100ml of sterilized saline, and mix thoroughly to obtain a 1:10000 dilution with mold (this dilution contains approximately 100 mold colonies per ml)...

Embodiment 2

[0033] The inhibitory effect of embodiment 2 sodium butylate to six different molds

[0034] Six kinds of standard experimental bacteria: Aspergillus flavus, Aspergillus fumigatus, Penicillium solanum, Penicillium inmanica, Fusarium moniliforme and Fusarium graminearum were purchased from China General Microbiology Collection Center.

[0035] 1.1 Strain recovery culture

[0036] Use a sterile pipette to draw 0.3-0.4ml of a suitable liquid medium glucose-peptone soup, drop it into an ampoule tube with the corresponding strain, and shake gently to dissolve the freeze-dried bacteria into a suspension; take about 0.2ml of bacteria Somatic suspension, transplanted on the designated agar slant / plate medium, cultured for 5 days.

[0037] 1.2 Plate preparation

[0038] Take 0.1g, 0.125g, 0.15g, 0.175g, and 0.2g of sodium butylate respectively in a 250ml sterilized Erlenmeyer flask, add 100ml of sterilized normal saline, place the shaker for 30min, take it off, and put it in an elect...

Embodiment 3

[0041] The influence of embodiment 3pH value on inhibitory effect of sodium butylate

[0042] 1.1 Preparation of media with different pH values

[0043] Adjust the pH of the culture medium (species) to 5.5 and 7.5 with 0.1 mol / L sodium hydroxide.

[0044]1.2 Preparation of plates: take 0.1g, 0.125g, 0.15g, 0.175g, 0.2g, 0.225g, 0.25g, 0.275g of sodium butylate (Jiangsu Tiancheng Biochemical Products Co., Ltd. g, 0.3g, 0.325g, and 0.35g are placed in a 250ml sterilized Erlenmeyer flask, add 100ml of sterilized normal saline, put it on the shaker for 30min, take it off, put it on an electric furnace and heat it to about 50°C for 3-5min , to fully dissolve sodium butyrate, add 1ml each to a sterile petri dish, pour 19ml of each pH medium sterilized at about 45°C, mix well and wait for it to solidify, the final concentration is: 40mg / kg, 50mg / kg kg, 60mg / kg, 62mg / kg, 88mg / kg, 90mg / kg, 100mg / kg, 110mg / kg, 120mg / kg, 130mg / kg, 140mg / kg. A control group of each pH value was set up,...

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Abstract

The invention provides an application of sodium butyl in mould proof of feeds, which is used for preventing and curing aspergillus flavus, aspergillus fumigatus, penicillium curinum thom, penicilliumislandicum, fusarium moniliforme sheld, fusarium graminearum or the mixed mould which is extracted from the moulding feeds. The minimum bacteriostat concentration of the sodium butyl to the mixed mould is 62mg / kg; the minimum bacteriostat concentration to the aspergillus flavus and aspergillus fumigatus is 62mg / kg; the minimum bacteriostat concentration to the penicillium curinum thom, penicilliumislandicum, fusarium moniliforme sheld and fusarium graminearum is 88mg / kg; after being processed for 30 minutes at the high temperature of 121 DEG C, the minimum bacteriostat concentration of the sodium butyl is 62mg / kg; and when the pH value respectively equals to 5.5 and 7.5, the minimum bacteriostat concentrations of the sodium butyl are respectively 88mg / kg, 130mg / kg and 140mg / kg. The invention provides the bacteriostat concentration of the sodium butyl to several types of moulds under different conditions, and lays a foundation for the sodium butyl to be served as mould proof agent in feed production.

Description

technical field [0001] The present invention relates to the technical field of antifungal agents, more specifically, to the technical field of antifungal agents for feed, especially the application of sodium butylate in antifungal for feed. Background technique [0002] During the storage, transportation, sale and use of feed, due to the change of moisture in the feed and the influence of the environment, it is very prone to mildew, and a large number of molds that grow and reproduce pollute the feed. Moldy feed and mycotoxin poisoning are the most common diseases in livestock worldwide. It is a common serious problem in poultry production, and the control of feed mildew is a link that cannot be ignored in feed production. [0003] Currently commonly used antifungal agents include propionic acid and propionate, sorbic acid and its potassium salt, benzoic acid and its sodium salt, and sodium diacetate. These fungicides play a very important role in protecting feed quality an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K3/03A23K1/00A23K30/18
Inventor 胡金来
Owner 胡金来
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