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Enzyme bioelectrochemical sensing chip and preparation method thereof

A bioelectrochemical and sensor chip technology, applied in the field of enzyme bioelectrochemical sensor chip, the preparation of the biosensor chip, can solve the problems of increasing the difficulty and cost of biosensor chip manufacturing, and achieve rapid electron transfer, Improve detection sensitivity and good interception effect

Inactive Publication Date: 2010-02-17
WUXI AIKANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These have increased the difficulty and cost of manufacturing biosensor chips

Method used

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  • Enzyme bioelectrochemical sensing chip and preparation method thereof
  • Enzyme bioelectrochemical sensing chip and preparation method thereof
  • Enzyme bioelectrochemical sensing chip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 Preparation of Micro / Nano Porous Biosensor Chip Working Electrode 1

[0068] The conductive ink used in this embodiment is carbon ink. The formula and ratio of carbon ink are listed in Table 1.

[0069] Table 1

[0070] Material

weight percentage

Carboxyl-containing linear polymer A (hydrolyzed styrene and horse

Anhydride-based copolymer) 35%-45% aqueous solution

45-65

carbon black particles

10-15

Graphite particles

15-20

Tween80 (Tween 80) dispersant

2-10

water

0-10

[0071] The carbon ink prepared according to the formula and ratio in Table 1 was screen printed and dried to form a micro-nano porous conductive layer attached to the surface of the polyester base insulating sheet. Its thickness is 5-50 microns. The drying temperature and time are 55-125° C. and 5-30 minutes, respectively.

[0072] The polymer dielectric is obtained by reacting vinylferrocene carbox...

Embodiment 2

[0075] Example 2 Testing of Working Electrode 1 of Micro / Nano Porous Biosensor Chip

[0076] The biosensor chip was composed of the working electrode 1 of the biosensor chip prepared in Example 1 and an Ag / AgCl reference electrode prepared by screen printing. The test of the glucose response current is to use a stabilized potentiometer connected with a computer to maintain a stable potential of 0.4 volts. The two electrodes are fixed side by side in a small flow cell with an inlet and outlet. PBS with different glucose concentrations (pH 7.4) continuously flows over the surfaces of the two electrodes at a flow rate of 10-15 cm / min, and continuously records the changes of the biosensor chip to glucose and its concentration. The glucose concentration was regularly calibrated with YSI (YSI company, YellowSprings blood glucose analyzer).

[0077] image 3 Display test results: the response current of the sensor chip varies with the concentration of glucose in PBS.

Embodiment 3

[0078] Example 3 Preparation of Micro / Nano Porous Biosensor Chip Working Electrode 2

[0079] Except for the materials used, the preparation process and method of the working electrode 2 of the micro-nano porous biosensor chip are the same as those in Example 1.

[0080] The polymer dielectric here is composed of ethylene ferrocene (vinylferrocene), methacrylic acid-2-diethylamine ethyl ester (2-(diethylammonia)ethyl methacrylate) and methacrylic acid-2-hydroxyethyl ester (2-(hydroxyethyl)methacrylate) is formed by free radical copolymerization. See reference 12 of this patent for details. The biological enzyme is glucose dehydrogenase.

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Abstract

The invention relates to an enzyme bioelectrochemical sensing chip and a preparation method thereof, belonging to the technical fields of analysis and detection. In the invention, the screen printingtechnique which is suitable for large-scale batch production is used for preparing a conducting layer which is attached to a substrate insulating material and has a micro-nano hole structure, and thesuperimposed molecular adsorption layer technique is used for trapping high molecular dielectrics and biological enzymes in the micro-nano hole to prepare a working electrode of a biological sensing chip for quantitatively delectating solute molecules (such as glucose, cholesterol, blood ketone or lactic acid) in liquid samples (such as blood), and the sensing chip is especially suitable for the quantitative detection by the continuous method. The high molecular dielectrics and the biological enzymes in the biological sensing chip of the invention are in contact with each other in a moleculardispersed state and are tightly close to conducting particles, thus the quick electron transfer can be achieved to be favorable for improving the detection sensitivity of the sensing chip; and the superimposed molecular adsorption layer technique is used for trapping the high molecular dielectrics and the biological enzymes, thus the semi-dialysis membrane has no need of being used singly, and theintercepting effect is better.

Description

technical field [0001] The invention relates to an enzyme bioelectrochemical sensor chip for detecting the content of a specific solute in a liquid, and also relates to a preparation method of the biosensor chip, which belongs to the technical field of analysis and detection, including medical treatment, environmental protection, food safety and the like. Background technique [0002] Enzyme bioelectrochemical sensor is a new type of analysis and detection equipment, which has the characteristics of high precision, strong selectivity, fast speed, small size, low cost, simple operation and convenient use. Therefore, it has great application potential and development prospects in many fields, such as health care, environmental protection and food safety. Enzyme bioelectrochemical sensor chip (abbreviated as biosensor chip) is the core component of enzyme bioelectrochemical sensor. It is mainly composed of two parts: molecular receptor and signal sensor. The former is usually...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N33/92G01N33/66G01N33/64C09J133/00C09J175/04C09J163/00C09D11/10C09D11/52
Inventor 刘丹
Owner WUXI AIKANG BIOTECH
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