Screening method of lincomycin producing strain

A screening method, lincomycin technology, applied in biochemical equipment and methods, microbial measurement/testing, mutant preparation, etc., can solve the problems of little increase in lincomycin yield and unsatisfactory effect, etc. Accelerate the process, expand the scale, and improve the efficiency of breeding

Inactive Publication Date: 2010-02-03
南阳普康药业有限公司
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for screening high-yielding lincomycin-producing strains include: treating lincomycin-producing bacteria with ultraviolet light / lithium chloride compound mutagen, and screening the strains with high-efficiency agar block culture method. Results: Screened out The fermentation unit of the strain in the shake flask was increased by 21.73% compared with the control strain; while the lincomycin-producing bacteria were treated with thermal mutagenesis, and the strain was screened by the efficient agar block culture method, the result: the screened strain The fermentation unit in the shake flask was increased by 23.53% compared with the control strain; these methods for screening high-yielding strains of lincomycin did not improve the yield of lincomycin much, and the effect was still not ideal

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Screening method of lincomycin producing strain
  • Screening method of lincomycin producing strain
  • Screening method of lincomycin producing strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] The screening method of the present invention is as follows:

[0017] Put the frozen-preserved sand tube into the slant and cultivate it to maturity, then use it as the starting strain, scrape off the appropriate amount of spores, dilute with sterile water, and inject N + Carry out mutagenesis treatment, culture on a medium containing methionine, use agar block culture method for primary screening, use primary and secondary fermentation medium for secondary screening, and carry out genetic stability test for the selected high-yield mutant strains , to obtain the target strain.

[0018] The mutagen N + The injection dosage is 5-50keV, and the concentration percentage of the resistant substance methionine is 0.1-4.0%.

[0019] The primary fermentation medium is made of the following raw materials in weight percentage: starch 1.0-3.0%, soybean powder 1.0-3.0%, peptone 0.3-0.7%, glucose 0.5-1.5%, KNO 3 0.1-0.3%, NaH 2 PO 4 0.4-1.0%, CaCO 3 0.1-0.5%, the balance is wat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a screening method of lincomycin producing strain. In the screening method, composite treatment of mutagenesis and resistance screening is conducted to streptomyces lincolnensis of the lincomycin producing strain by adopting an ion implantation technology, N<+> is used as an ion source, the biological effect of N<+> implantation on the lincomycin producing strain is studied, and a lincomycin precursor which is the product of the lincomycin producing strain is used for obtaining a plurality of high-yield mutant strains with better genetic stability by the resistance screening method, so as to lead the fermentation unit of the mutant strains to be improved by 81.4% than that of original strains.

Description

Technical field [0001] The invention relates to a screening method for lincomycin-producing bacteria, in particular to a screening method for mutant bacteria that can increase the yield of lincomycin. Background technique [0002] Lincomycin, also known as lincomycin, is a lincosamide antibiotic produced by Streptomyces lincolnensis. Its components include amino acids and sugars. It is produced by L-tyrosine and pentose phosphorus cycle (pentose -5-phosphate and sedum sugar-7-phosphate) condensation intermediates, the whole biosynthetic pathway includes two branches: one is synthesized from L-tyrosine to propylproline (PPL), and the other is synthesized from sedum Tenose-7-phosphate constitutes α-methylthiolincosamide (α-MIL), which is produced through three stages of aerobic fermentation. Anti-Gram-positive bacteria, clinically mainly used to treat infections caused by Gram-positive bacteria, has the characteristics of significant curative effect, less side effects, and le...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C12Q1/04
Inventor 李红德高同伟张新静冯剑波赵晴王锋刘海波
Owner 南阳普康药业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap