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Screening method of lincomycin producing strain

A screening method, lincomycin technology, applied in biochemical equipment and methods, microbial measurement/testing, mutant preparation, etc., can solve the problems of little increase in lincomycin yield and unsatisfactory effect, etc. Accelerate the process, expand the scale, and improve the efficiency of breeding

Inactive Publication Date: 2010-02-03
南阳普康药业有限公司
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Problems solved by technology

[0003] At present, the methods for screening high-yielding lincomycin-producing strains include: treating lincomycin-producing bacteria with ultraviolet light / lithium chloride compound mutagen, and screening the strains with high-efficiency agar block culture method. Results: Screened out The fermentation unit of the strain in the shake flask was increased by 21.73% compared with the control strain; while the lincomycin-producing bacteria were treated with thermal mutagenesis, and the strain was screened by the efficient agar block culture method, the result: the screened strain The fermentation unit in the shake flask was increased by 23.53% compared with the control strain; these methods for screening high-yielding strains of lincomycin did not improve the yield of lincomycin much, and the effect was still not ideal

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  • Screening method of lincomycin producing strain
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  • Screening method of lincomycin producing strain

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Embodiment Construction

[0016] The screening method of the present invention is as follows:

[0017] Put the frozen-preserved sand tube into the slant and cultivate it to maturity, then use it as the starting strain, scrape off the appropriate amount of spores, dilute with sterile water, and inject N + Carry out mutagenesis treatment, culture on a medium containing methionine, use agar block culture method for primary screening, use primary and secondary fermentation medium for secondary screening, and carry out genetic stability test for the selected high-yield mutant strains , to obtain the target strain.

[0018] The mutagen N + The injection dosage is 5-50keV, and the concentration percentage of the resistant substance methionine is 0.1-4.0%.

[0019] The primary fermentation medium is made of the following raw materials in weight percentage: starch 1.0-3.0%, soybean powder 1.0-3.0%, peptone 0.3-0.7%, glucose 0.5-1.5%, KNO 3 0.1-0.3%, NaH 2 PO 4 0.4-1.0%, CaCO 3 0.1-0.5%, the balance is wat...

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Abstract

The invention discloses a screening method of lincomycin producing strain. In the screening method, composite treatment of mutagenesis and resistance screening is conducted to streptomyces lincolnensis of the lincomycin producing strain by adopting an ion implantation technology, N<+> is used as an ion source, the biological effect of N<+> implantation on the lincomycin producing strain is studied, and a lincomycin precursor which is the product of the lincomycin producing strain is used for obtaining a plurality of high-yield mutant strains with better genetic stability by the resistance screening method, so as to lead the fermentation unit of the mutant strains to be improved by 81.4% than that of original strains.

Description

Technical field [0001] The invention relates to a screening method for lincomycin-producing bacteria, in particular to a screening method for mutant bacteria that can increase the yield of lincomycin. Background technique [0002] Lincomycin, also known as lincomycin, is a lincosamide antibiotic produced by Streptomyces lincolnensis. Its components include amino acids and sugars. It is produced by L-tyrosine and pentose phosphorus cycle (pentose -5-phosphate and sedum sugar-7-phosphate) condensation intermediates, the whole biosynthetic pathway includes two branches: one is synthesized from L-tyrosine to propylproline (PPL), and the other is synthesized from sedum Tenose-7-phosphate constitutes α-methylthiolincosamide (α-MIL), which is produced through three stages of aerobic fermentation. Anti-Gram-positive bacteria, clinically mainly used to treat infections caused by Gram-positive bacteria, has the characteristics of significant curative effect, less side effects, and le...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C12Q1/04
Inventor 李红德高同伟张新静冯剑波赵晴王锋刘海波
Owner 南阳普康药业有限公司
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