Preparation method of chitosan asymetric membrane for leading periodontium to be regenerated
A periodontal tissue regeneration and asymmetric membrane technology, which is applied in the field of preparation of chitosan asymmetric membranes, can solve the problems of expensive periodontal tissue regeneration materials, blank periodontal regeneration materials, and influence on the quality of osteogenesis, and achieves good Osteoinductive, low cost, good histocompatibility
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Embodiment 1
[0014] 1) Preparation of chitosan matrix film: add chitosan powder with a viscosity average molecular weight of 200,000 to a 2% acetic acid solution, and stir vigorously for 2 hours to obtain a 3% chitosan solution. Let stand for 24h to degas. The defoamed chitosan solution was cast on a glass plate to form a film, and after being treated with sodium hydroxide coagulation solution, it was dried at 50°C to obtain a chitosan matrix film with a thickness of 15 μm.
[0015] 2) Preparation of spinning solution A: dissolving chitosan powder with a viscosity average molecular weight of 200,000 in an acetic acid solution with a volume fraction of 70%. Polyvinyl alcohol (degree of polymerization: 1750±50) was dissolved in 90°C deionized water and cooled. The chitosan acetic acid solution and the polyvinyl alcohol aqueous solution are uniformly mixed, the mass ratio of chitosan: polyvinyl alcohol is 60:40, a mixed solution with a total concentration of 7% is prepared, and the spinning solut...
Embodiment 2
[0019] 1) Preparation of chitosan matrix film: add chitosan powder with a viscosity-average molecular weight of 560,000 to a 2% acetic acid solution and stir vigorously for 1 hour to obtain a 5% chitosan solution. Let stand for 24h to degas. The defoamed chitosan solution was cast on a glass plate to form a film, which was treated with sodium hydroxide coagulation solution and dried at 70° C. to obtain a chitosan matrix film with a thickness of 30 μm.
[0020] 2) Preparation of spinning solution A: Dissolve chitosan powder with a viscosity average molecular weight of 560,000 in an acetic acid solution with a volume fraction of 80%. Polyvinyl alcohol (degree of polymerization: 1750±50) was dissolved in 90°C deionized water and cooled. The chitosan acetic acid solution and the polyvinyl alcohol aqueous solution are uniformly mixed, the mass ratio of chitosan: polyvinyl alcohol is 80:20, a mixed solution with a total concentration of 10% is prepared, and the spinning solution A is ob...
Embodiment 3
[0024] 1) Preparation of chitosan matrix film: add chitosan powder with a viscosity average molecular weight of 750,000 to a 2% acetic acid solution and stir vigorously for 2 hours to obtain a 6% chitosan solution. Let stand for 24h to degas. The defoamed chitosan solution was cast on a glass plate to form a film, and after being treated with sodium hydroxide coagulation solution, it was dried at 80°C to obtain a chitosan matrix film with a thickness of 40 μm.
[0025] 2) Preparation of spinning solution A: Dissolve chitosan powder with a viscosity average molecular weight of 750,000 in an acetic acid solution with a volume fraction of 90%. Polyvinyl alcohol (degree of polymerization: 1750±50) was dissolved in 90°C deionized water and cooled. The chitosan acetic acid solution and the polyvinyl alcohol aqueous solution are uniformly mixed, the mass ratio of chitosan: polyvinyl alcohol is 90:10, a mixed solution with a total concentration of 5% is prepared, and the spinning solution...
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