Composite strain microbial dry powder for bio-pulping and environment-friendly energy-saving type composite strain bio-pulping process
A composite strain and biological pulping technology, applied in the direction of microorganisms, microorganism-based methods, cellulose raw material pulping, etc., can solve the damage of pulp tensile strength, burst strength and tear strength, and straw resources cannot be used Papermaking, pollution control and other difficult problems, to achieve the effect of improving the bursting strength and tear strength, improving the comprehensive productivity of the soil, and overcoming the damage of pulp performance
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Embodiment 1
[0044] 1. The preparation method of composite bacterial strain microbial dry powder for straw bio-pulping, including three steps of seed culture, industrial production of original seed, and mixing and drying.
[0045] 1. Seed cultivation:
[0046] Yeast culture:
[0047] The lipolytic yeasts on each purified and cultivated slant medium: Saccharomyces cerevisiae, Candida lipolytica, Hansenula heteroformes, and Saccharomyces cerevisiae were inoculated into 2L culture bottles, and the bottles contained 0.4kg of wort potato sucrose medium , three of each, cultured for 36 hours at a culture temperature of 36-42°C and a shaker rotation speed of 140-220 rpm. The wort potato sucrose medium is a conventional medium, and its dosage is the conventional dosage.
[0048] Cellulose isolation bacteria culture:
[0049] The fiber isolates on the slant medium of each purified culture: Mycobacterium budding, Trichoderma konziii, white rot fungus, and pure ascomycetes were inoculated into 2L ...
Embodiment 2
[0069] The fermentation device can be changed from a fermentation tank to a continuous fermentation tunnel. The raw meal that is sprayed and mixed with pulping microorganisms is quantitatively loaded every day, and is fed into the fermentation tunnel by rail. After several days, the corresponding straw fermentation is completed every day, and the clinker is also output from the tunnel by the rail for pulping. Other processes and examples 1 is the same.
Embodiment 3
[0071] The preparation method of microbial dry powder for pulping other straws and woody plant raw materials is roughly the same as that of Example 1. The amount of microbial dry powder depends on the waxy layer and the amount of cellulose, lignin and colloid contained in the raw materials. , Cellulose isolates and their respective small bacteria weight ratios are optimized and adjusted. The principle is that the amount of lipolytic yeast with less waxy layer is relatively reduced, and the amount of fiber isolates with more cellulose, lignin and glial is relatively increased.
[0072] The related strains of Saccharomyces cerevisiae, Candida lipolytica, Hansenula heteromorpha, Saccharomyces cerevisiae, Mycobacterium bud, White rot fungus, Trichoderma kangseri, and Ascomycetes involved in the present invention can be entrusted to relevant microbial research institutions to help screen isolated culture. After the bacterial seed source is obtained, it is then cultivated according ...
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