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Method for extracting L-arabinose from xylose mother liquor and biomass acid hydrolysis solution

A technology for xylose mother liquor and acid hydrolyzate, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, sugar derivatives, etc., can solve the problems of consumption of xylose, low extraction purity, and no indication, and achieves good results. The effect of stability

Inactive Publication Date: 2009-02-25
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the method needs to be treated with amylase in advance, the operation steps and production cost are increased, and there is a certain amount of xylose after hydrolysis, and the used baker's yeast or brewer's yeast itself cannot metabolize and consume xylose.
However, the invention does not indicate whether the bread or brewer's yeast used is a genetically engineered baker's yeast that can metabolize xylose
[0010] In short, the production of L-arabinose by relying on the existing technology has the disadvantages of high production cost, unfriendly environment and low extraction purity, so it is very important to develop a cost-effective extraction method to reduce production cost

Method used

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  • Method for extracting L-arabinose from xylose mother liquor and biomass acid hydrolysis solution
  • Method for extracting L-arabinose from xylose mother liquor and biomass acid hydrolysis solution
  • Method for extracting L-arabinose from xylose mother liquor and biomass acid hydrolysis solution

Examples

Experimental program
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Effect test

Embodiment 1

[0053]Measure 1000ml xylose mother liquor (from Shandong Futian Pharmaceutical Co., Ltd.), this mother liquor is the light brown viscous liquid that remains after xylose is crystallized with corncob hydrolyzate, and this mother liquor contains xylose 50-70%, L-Arabic Sugar 15-20%, glucose 10-15%, galactose 3-8% and other miscellaneous sugars below 7% (all by mass percent of dry matter), the HPLC analysis of this mother liquor is as follows figure 1 Shown: the composition of the xylose mother liquor is very complex, among which xylose accounts for more than 50%, L-arabinose accounts for about 20%, glucose accounts for about 15%, and galactose accounts for about 10%. Adding mass percentage is 1% yeast powder (produced by OXOID company), mass percentage is 0.01% anhydrous magnesium sulfate and mass percentage is 0.1% potassium dihydrogen phosphate, dilute to 2.7L with deionized water (mother liquor is diluted 3 times ), fully dissolved, transferred to a 10L fermenter, and sterili...

Embodiment 2

[0055] Measure 1000ml of xylose mother liquor (from Shandong Futian Pharmaceutical Co., Ltd.), add 0.1% yeast powder (produced by OXOID company), 0.1% bean cake powder and 0.01% anhydrous magnesium sulfate and 0.04% potassium dihydrogen phosphate, Dilute to 4500ml (mother liquor is diluted 5 times) with deionized water, fully dissolve, transfer to a 10-liter fermenter, and sterilize off-site, and the sterilization condition is sterilizing at 115° C. for 30 minutes. Inoculate Debariae CGMCC 2480 stored on the slant of the test tube into a 50 ml test tube containing 5 ml of fermentation medium, cultivate at 36 ° C, and cultivate for 12 hours at a stirring speed of 150 rpm to obtain a primary seed liquid; Add all 2.5ml of the primary seed solution into a 500ml shake flask containing 50ml of fermentation medium, cultivate at 35°C, and cultivate for 12 hours at a stirring speed of 250rpm to obtain the secondary seed solution; add all the 25ml of the secondary seed solution Cultivat...

Embodiment 3

[0057] Measure 700ml of xylose mother liquor (from Shandong Changyi Jiahe Sugar Industry Co., Ltd.), this mother liquor is the dark brown viscous liquid that remains after crystallizing xylose with corncob hydrolyzate, and this mother liquor contains xylose 45-70%, 10-20% of L-arabinose, 10-20% of glucose, 3-8% of galactose and less than 7% of other miscellaneous sugars (all in terms of dry matter mass percentage). Add 3% yeast powder (manufactured by Hubei Angel Co., Ltd.) and 0.1% anhydrous magnesium sulfate and 0.2% potassium dihydrogen phosphate, dilute to 6.3 liters with deionized water (dilute the mother liquor 10 times), fully dissolve , transferred to a 10-liter fermenter, and sterilized off-site at a temperature of 115°C for 20 minutes. Inoculate Debariae CGMCC 2480 stored on the slant of the test tube into a 50 ml test tube containing 5 ml of fermentation medium, cultivate at 30°C, and cultivate for 36 hours at a stirring speed of 350 rpm to obtain a primary seed liq...

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Abstract

The invention discloses a method for extracting L-arabinose from xylose mother liquor and biomass acid hydrolysate, which mainly comprises the following steps: step 1: the xylose mother liquor and the biomass are pretreated; step 2: Debaryomyces yeast Y161 (Debaryomyces, spp) is cultured and collected in China General Microbiological Culture Collection Center (CGMCC) with the culture collection number being 2480; step 3: the cultured yeast is inoculated in the xylose mother liquor or the hydrolysate for culture; step 4: the fermentation liquor obtained is decolorized, and then ion exchange is carried out on the decolorized fermentation liquor; step 5: the fermentation liquor is condensed until the sugar content is more than 70%; step 6: anhydrous ethanol and L-arabinose seed crystals are added for crystallization; step 7: the crystals obtained are washed by 95% of ethanol ,and then dried to obtain the pure L-arabinose product. The method has the advantages of no pollution, low cost and low energy consumption, and is suitable for the large-scale industrialized production of the L-arabinose.

Description

technical field [0001] The invention relates to a method for extracting sugar in the field of biotechnology, in particular to a method for extracting L-arabinose from xylose mother liquor and biomass acid hydrolyzate. Background technique [0002] L-arabinose, also known as pectin sugar, exists in large quantities in plant pulp, hemicellulose, Pectic acid rarely exists in free form. L-arabinose is a new type of functional sugar. A large number of studies have proved that it can inhibit the activity of sucrase in the intestinal tract of the body, greatly reduce the absorption of sucrose intake and the secretion of insulin, thereby reducing the occurrence of obesity and diabetes. In sucrose Adding 3-4% L-arabinose can significantly reduce blood sugar levels within 30 minutes after eating (Metabolism, Vol 45, No 11, 1996: pp1368-1374; Journal of Nutrition, Vol 131, No 3, 2001: pp796-799; Journal of Japanese Society of Nutrition and Food Science, Vol 53, No 6, 2000: pp243-247)...

Claims

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Application Information

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IPC IPC(8): C12P19/02C07H1/06C12R1/645
Inventor 邓子新程海荣
Owner SHANGHAI JIAO TONG UNIV
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