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Wheat sharp eyespot resistance seedling identification method

A technology for wheat sheath blight and wheat sheath blight fungus is applied in the field of identification of resistance to wheat sheath blight, which can solve the problems of large workload, environmental impact, long identification time, etc., and achieves elimination of differences in pathogenicity, The effect of improving utilization efficiency and short identification cycle

Inactive Publication Date: 2009-01-21
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to: for the current identification of wheat sheath blight resistance, it is easy to be affected by the environment, and the evaluation standards are not uniform, the identification time is long, the workload is large, and the pathogenic bacteria of the blight currently used are different and the pathogenicity is different. To provide a new system for identification of wheat sheath blight resistance at seedling stage

Method used

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  • Wheat sharp eyespot resistance seedling identification method
  • Wheat sharp eyespot resistance seedling identification method
  • Wheat sharp eyespot resistance seedling identification method

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Pathogen of sheath blight: Rhizactonia cerealis strain provided by Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, the strain numbered by the Institute of Plant Protection is strain R0301 (the same below).

[0022] The bacterial strain R0301 was activated and cultured on the PDA solid medium on the ultra-clean workbench, and it was used after 7 days.

[0023] Put the toothpick bought in the market into the box upright and neatly after being folded in half, soak for 24 hours, add the dissolved PDA solid medium into the box to immerse 1 / 4 of the toothpick; The wheat sheath blight strain cultured in the plate was aseptically moved into the PDA solid medium containing toothpicks, and then placed in a 25°C incubator for cultivation. After the toothpicks were covered with hyphae, a toothpick with bacteria was formed for use.

Embodiment 2

[0025] The identified variety: CI12633, imported from the United States, provided by the Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences.

[0026] Bacterial toothpick: obtained through Example 1.

[0027] Seeds of wheat CI12633 were germinated in petri dishes at 25°C and then sown in 20cm nutrient pots for growth. 14 seeds were sown in each nutrient pot. During the growth period, the temperature was kept at 20-25°C during the day and 10-15°C at night.

[0028] 30 days after sowing, use sterilized tweezers to pick up the sterile toothpick and gently insert it into the stems and leaf sheaths of wheat seedlings. Insert a sterile toothpick into each leaf sheath. Wrap the soaked medical absorbent cotton balls around the inoculation site and cover them with double-layer sunshade nets. Keep the inoculated seedlings at nearly 100% saturated relative humidity for 48 hours through a mist diffuser, then remove the sunshade net, and manage the normal growth of the see...

Embodiment 3

[0036] Utilize the sterile toothpick obtained in Example 1, adopt the same identification method as in Example 2, to ARz, red clam, Ziganzi, Cooker983, Limai 16, Huamai No. 8, Yangmai 158, Zhen74-95, Nine wheat varieties including Yemao were identified at seedling stage for sheath blight resistance, and the identification results are recorded in Table 1. The nine wheat varieties were all provided by the Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences.

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Abstract

The invention relates to a method for identifying a resistance seedling stage against wheat banded sclerotial blight. The method comprises the following steps that Rhizactonia cerealis undergoes activated cultivation to obtain hypha; the hypha grows over a toothpick through a culture medium so as to form a nonsterile toothpick; seeds of to-be-identified wheat variety or strain sprout inside a culture dish at a temperature of 25 DEG C, and then are sown inside a 20cm nutrition pot; the nonsterile toothpick is embedded in a wheat seedling stalk and a leaf sheath 30 days after the seeds are sown, and an inoculation position is wound with soaked medical absorbent cotton tampon and is covered with a double-layer shading net; inoculated seedling is kept at a relative humidity and near 100 percent saturation for 48 hours by means of a mist sprayer, and then the shading net is removed; the seedling undergoes normal growth management, and water spray is carried out once a day; and grade identification of banded sclerotial blight disease for individual wheat material is carried out 30 days after inoculation; and finally, disease index is calculated according to disease evaluation grade.

Description

technical field [0001] The present invention relates to a method for identifying resistance to wheat sheath blight, in particular to quickly and effectively screen wheat sheath blight resistance by identifying wheat varieties or strains of wheat sheath blight resistance at the seedling stage under environmental control conditions. source to improve the efficiency of wheat sheath blight resistance breeding. The invention belongs to the technical field of plant protection. Background technique [0002] Wheat sheath blight, also known as wheat sharp eyespot, is a soil-borne fungal disease caused by Rhizoctonia cerealis or Rhizactonia solani in Rhizoctonia (Chen Yanxi, 1986; Xia Zhengjun, 1989), almost Throughout the wheat-growing regions of the world. The occurrence and damage of wheat sheath blight have been reported in more than 10 countries including Asia, Europe, America, and Australia (Glynne, 1934; Polley, 1991; Pasquini, 1996; Zoltanska, 1997; Zhou Kainan, 1982; Chen S...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02A01H1/00
Inventor 任丽娟周淼平张旭余桂红马鸿翔
Owner JIANGSU ACAD OF AGRI SCI
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